Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites

Vip3Aa, Vip3Af, Cry1Ab, and Cry1Fa were tested for their toxicities and binding interactions. Vip3A proteins were more toxic than Cry1 proteins. Binding assays showed independent specific binding sites for Cry1 and Vip3A proteins. Cry1Ab and Cry1Fa competed for the same binding sites, whereas Vip3Aa...

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Authors: Sena, Janete A. D. [UNESP], Hernández-Rodríguez, Carmen Sara, Ferré, Juan
Format: article
Status:Published version
Publication Date:2009
Country:Brasil
Institution:Universidade Estadual Paulista (UNESP)
Repository:Repositório Institucional da UNESP
Language:English
OAI Identifier:oai:repositorio.unesp.br:11449/70965
Online Access:http://dx.doi.org/10.1128/AEM.02342-08
http://hdl.handle.net/11449/70965
Access Level:Open access
Keyword:Bacillus thuringiensis
Binding assays
Binding interactions
Specific bindings
Spodoptera frugiperda
Bacteriology
Binding energy
Proteins
Binding sites
Bacillus thuringiensis toxin
bacterial toxin
Cry1Ab toxin
cry1fa protein
unclassified drug
vip3aa protein
vip3af protein
bacterium
caterpillar
protein
toxicity
bacterial strain
binding site
bioassay
Lepidoptera
midgut
nonhuman
protein analysis
protein expression
protein protein interaction
Animals
Bacterial Proteins
Endotoxins
Gastrointestinal Tract
Hemolysin Proteins
Larva
Lethal Dose 50
Protein Binding
Spodoptera
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spelling Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sitesBacillus thuringiensisBinding assaysBinding interactionsSpecific bindingsSpodoptera frugiperdaBacteriologyBinding energyProteinsBinding sitesBacillus thuringiensis toxinbacterial toxinCry1Ab toxincry1fa proteinunclassified drugvip3aa proteinvip3af proteinbacteriumcaterpillarproteintoxicitybacterial strainbinding sitebioassayLepidopteramidgutnonhumanprotein analysisprotein expressionprotein protein interactionAnimalsBacterial ProteinsEndotoxinsGastrointestinal TractHemolysin ProteinsLarvaLethal Dose 50Protein BindingSpodopteraVip3Aa, Vip3Af, Cry1Ab, and Cry1Fa were tested for their toxicities and binding interactions. Vip3A proteins were more toxic than Cry1 proteins. Binding assays showed independent specific binding sites for Cry1 and Vip3A proteins. Cry1Ab and Cry1Fa competed for the same binding sites, whereas Vip3Aa competed for those of Vip3Af. Copyright © 2009, American Society for Microbiology. All Rights Reserved.Departamento de Genética Facultad de CC. Biológicas Universidad de Valencia, ValenciaDepartamento de Genética Facultad de CC. Biológicas, Dr. Moliner 50, 46100-Burjassot, ValenciaUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia Aplicada a Agropecuaria, Jaboticabal, São PauloUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia Aplicada a Agropecuaria, Jaboticabal, São PauloUniversidad de ValenciaFacultad de CC. BiológicasUniversidade Estadual Paulista (Unesp)2014-05-27T11:23:53Z2014-05-27T11:23:53Z2009-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2236-2237application/pdfhttp://dx.doi.org/10.1128/AEM.02342-08Applied and Environmental Microbiology, v. 75, n. 7, p. 2236-2237, 2009.0099-22401098-5336http://hdl.handle.net/11449/7096510.1128/AEM.02342-082-s2.0-638491548062-s2.0-63849154806.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied and Environmental Microbiology3.6331,6841,684info:eu-repo/semantics/openAccessSena, Janete A. D. [UNESP]Hernández-Rodríguez, Carmen SaraFerré, Juan2025-10-22T12:08:32Zoai:repositorio.unesp.br:11449/70965Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-22T12:08:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
title Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
spellingShingle Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
Sena, Janete A. D. [UNESP]
Bacillus thuringiensis
Binding assays
Binding interactions
Specific bindings
Spodoptera frugiperda
Bacteriology
Binding energy
Proteins
Binding sites
Bacillus thuringiensis toxin
bacterial toxin
Cry1Ab toxin
cry1fa protein
unclassified drug
vip3aa protein
vip3af protein
bacterium
caterpillar
protein
toxicity
bacterial strain
binding site
bioassay
Lepidoptera
midgut
nonhuman
protein analysis
protein expression
protein protein interaction
Animals
Bacterial Proteins
Endotoxins
Gastrointestinal Tract
Hemolysin Proteins
Larva
Lethal Dose 50
Protein Binding
Spodoptera
title_short Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
title_full Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
title_fullStr Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
title_full_unstemmed Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
title_sort Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
dc.creator.none.fl_str_mv Sena, Janete A. D. [UNESP]
Hernández-Rodríguez, Carmen Sara
Ferré, Juan
author Sena, Janete A. D. [UNESP]
author_facet Sena, Janete A. D. [UNESP]
Hernández-Rodríguez, Carmen Sara
Ferré, Juan
author_role author
author2 Hernández-Rodríguez, Carmen Sara
Ferré, Juan
author2_role author
author
dc.contributor.none.fl_str_mv Universidad de Valencia
Facultad de CC. Biológicas
Universidade Estadual Paulista (Unesp)
dc.subject.por.fl_str_mv Bacillus thuringiensis
Binding assays
Binding interactions
Specific bindings
Spodoptera frugiperda
Bacteriology
Binding energy
Proteins
Binding sites
Bacillus thuringiensis toxin
bacterial toxin
Cry1Ab toxin
cry1fa protein
unclassified drug
vip3aa protein
vip3af protein
bacterium
caterpillar
protein
toxicity
bacterial strain
binding site
bioassay
Lepidoptera
midgut
nonhuman
protein analysis
protein expression
protein protein interaction
Animals
Bacterial Proteins
Endotoxins
Gastrointestinal Tract
Hemolysin Proteins
Larva
Lethal Dose 50
Protein Binding
Spodoptera
topic Bacillus thuringiensis
Binding assays
Binding interactions
Specific bindings
Spodoptera frugiperda
Bacteriology
Binding energy
Proteins
Binding sites
Bacillus thuringiensis toxin
bacterial toxin
Cry1Ab toxin
cry1fa protein
unclassified drug
vip3aa protein
vip3af protein
bacterium
caterpillar
protein
toxicity
bacterial strain
binding site
bioassay
Lepidoptera
midgut
nonhuman
protein analysis
protein expression
protein protein interaction
Animals
Bacterial Proteins
Endotoxins
Gastrointestinal Tract
Hemolysin Proteins
Larva
Lethal Dose 50
Protein Binding
Spodoptera
description Vip3Aa, Vip3Af, Cry1Ab, and Cry1Fa were tested for their toxicities and binding interactions. Vip3A proteins were more toxic than Cry1 proteins. Binding assays showed independent specific binding sites for Cry1 and Vip3A proteins. Cry1Ab and Cry1Fa competed for the same binding sites, whereas Vip3Aa competed for those of Vip3Af. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-01
2014-05-27T11:23:53Z
2014-05-27T11:23:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1128/AEM.02342-08
Applied and Environmental Microbiology, v. 75, n. 7, p. 2236-2237, 2009.
0099-2240
1098-5336
http://hdl.handle.net/11449/70965
10.1128/AEM.02342-08
2-s2.0-63849154806
2-s2.0-63849154806.pdf
url http://dx.doi.org/10.1128/AEM.02342-08
http://hdl.handle.net/11449/70965
identifier_str_mv Applied and Environmental Microbiology, v. 75, n. 7, p. 2236-2237, 2009.
0099-2240
1098-5336
10.1128/AEM.02342-08
2-s2.0-63849154806
2-s2.0-63849154806.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Applied and Environmental Microbiology
3.633
1,684
1,684
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2236-2237
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1853672393327771648
score 15,812455

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