Metaloproteinases de matriz 2 e 9 no líquido cefalorraquidiano e soro de cães naturalmente infectados pelo vírus da cinomose
Canine distemper is an important contagious disease with high mortality rate and multisystem involvement including the central nervous system. The demyelinating leukoencephalitis is the main neuropathological alteration observed and its pathogenesis is not well known. The rupture of the blood brain...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.unesp.br:11449/121865 |
| Acceso en línea: | http://hdl.handle.net/11449/121865 |
| Access Level: | acceso abierto |
| Palabra clave: | Cão Encefalite Barreira hematoencefalica Cinomose Virus Canine distemper |
| Sumario: | Canine distemper is an important contagious disease with high mortality rate and multisystem involvement including the central nervous system. The demyelinating leukoencephalitis is the main neuropathological alteration observed and its pathogenesis is not well known. The rupture of the blood brain barrier (BBB) with viral penetration and leukocyte infiltration are crucial for the development of demyelinating encephalitis. In humans and animals with similar disorders, matrix metalloproteinases (MMPs) especially 2 and 9 have been pointed as the agents that destabilize the integrity of the BBB. Thus, we sought to establish the involvement of MMPs 2 and 9 in BBB rupture of dogs with distemper at the neurological phase. The present study aimed to determine and quantify these enzymes by the technique of zymography in serum and cerebrospinal fluid of healthy dogs (Gcon, n=10) and dogs with distemper at neurological stage (Gcin, n=10). Furthermore, we determined the physicochemical parameters of liquor to obtain more information about the integrity of the BBB. Exploratory multivariate analysis showed distinct patterns according to the variants studied, which were compared between groups with t test |
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