Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response
Background: Sleep is a physiological event that directly influences health by affecting the immune system, in which calcium (Ca2+) plays a critical signaling role. We performed live cell measurements of cytosolic Ca2+ mobilization to understand the changes in Ca2+ signaling that occur in splenic imm...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | Brasil |
| Institución: | Universidade Federal de São Paulo (UNIFESP) |
| Repositorio: | Repositório Institucional da UNIFESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/35515 |
| Acceso en línea: | http://dx.doi.org/10.1016/j.bbagen.2012.09.010 http://repositorio.unifesp.br/handle/11600/35515 |
| Access Level: | acceso abierto |
| Palabra clave: | Ca2+ signaling Splenocytes Sleep deprivation Mitochondrial dysfunction Mice |
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Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune responseCa2+ signalingSplenocytesSleep deprivationMitochondrial dysfunctionMiceBackground: Sleep is a physiological event that directly influences health by affecting the immune system, in which calcium (Ca2+) plays a critical signaling role. We performed live cell measurements of cytosolic Ca2+ mobilization to understand the changes in Ca2+ signaling that occur in splenic immune cells after various periods of sleep deprivation (SD).Methods: Adult male mice were subjected to sleep deprivation by platform technique for different periods (from 12 to 72 h) and Ca2+ intracellular fluctuations were evaluated in splenocytes by confocal microscopy. We also performed spleen cell evaluation by flow cytometry and analyzed intracellular Ca2+ mobilization in endoplasmic reticulum and mitochondria. Additionally. Ca2+ channel gene expression was evaluatedResults: Splenocytes showed a progressive loss of intracellular Ca2+ maintenance from endoplasmic reticulum (ER) stores. Transient Ca2+ buffering by the mitochondria was further compromised. These findings were confirmed by changes in mitochondrial integrity and in the performance of the store operated calcium entry (SOCE) and stromal interaction molecule 1 (STIM1) Ca2+ channels.Conclusions and general significance: These novel data suggest that SD impairs Ca2+ signaling, most likely as a result of ER stress, leading to an insufficient Ca2+ supply for signaling events. Our results support the previously described immunosuppressive effects of sleep loss and provide additional information on the cellular and molecular mechanisms involved in sleep function. (C) 2012 Elsevier B.V. All rights reserved.Universidade Federal de São Paulo UNIFESP, Dept Psychobiol, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Biosci, Santos, SP, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Biochem, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Psychobiol, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Biosci, Santos, SP, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Biochem, São Paulo, BrazilWeb of ScienceAFIP (Associacao Fundo de Incentivo a Pesquisa)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 05/04366-3FAPESP: CEPID 98/14303-6Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:28:02Z2016-01-24T14:28:02Z2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1997-2006application/pdfhttp://dx.doi.org/10.1016/j.bbagen.2012.09.010Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1820, n. 12, p. 1997-2006, 2012.10.1016/j.bbagen.2012.09.010WOS000311004000018.pdf0304-4165http://repositorio.unifesp.br/handle/11600/35515WOS:000311004000018ark:/48912/001300001vrgbengBiochimica Et Biophysica Acta-general Subjectsinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPLungato, Lisandro [UNIFESP]Gazarini, Marcos Leoni [UNIFESP]Paredes-Gamero, Edgar Julian [UNIFESP]Tersariol, Ivarne Luis dos Santos [UNIFESP]Tufik, Sergio [UNIFESP]D'Almeida, Vania [UNIFESP]2024-08-08T15:56:15Zoai:repositorio.unifesp.br:11600/35515Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T15:56:15Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response |
| title |
Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response |
| spellingShingle |
Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response Lungato, Lisandro [UNIFESP] Ca2+ signaling Splenocytes Sleep deprivation Mitochondrial dysfunction Mice |
| title_short |
Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response |
| title_full |
Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response |
| title_fullStr |
Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response |
| title_full_unstemmed |
Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response |
| title_sort |
Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response |
| dc.creator.none.fl_str_mv |
Lungato, Lisandro [UNIFESP] Gazarini, Marcos Leoni [UNIFESP] Paredes-Gamero, Edgar Julian [UNIFESP] Tersariol, Ivarne Luis dos Santos [UNIFESP] Tufik, Sergio [UNIFESP] D'Almeida, Vania [UNIFESP] |
| author |
Lungato, Lisandro [UNIFESP] |
| author_facet |
Lungato, Lisandro [UNIFESP] Gazarini, Marcos Leoni [UNIFESP] Paredes-Gamero, Edgar Julian [UNIFESP] Tersariol, Ivarne Luis dos Santos [UNIFESP] Tufik, Sergio [UNIFESP] D'Almeida, Vania [UNIFESP] |
| author_role |
author |
| author2 |
Gazarini, Marcos Leoni [UNIFESP] Paredes-Gamero, Edgar Julian [UNIFESP] Tersariol, Ivarne Luis dos Santos [UNIFESP] Tufik, Sergio [UNIFESP] D'Almeida, Vania [UNIFESP] |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
| dc.subject.por.fl_str_mv |
Ca2+ signaling Splenocytes Sleep deprivation Mitochondrial dysfunction Mice |
| topic |
Ca2+ signaling Splenocytes Sleep deprivation Mitochondrial dysfunction Mice |
| description |
Background: Sleep is a physiological event that directly influences health by affecting the immune system, in which calcium (Ca2+) plays a critical signaling role. We performed live cell measurements of cytosolic Ca2+ mobilization to understand the changes in Ca2+ signaling that occur in splenic immune cells after various periods of sleep deprivation (SD).Methods: Adult male mice were subjected to sleep deprivation by platform technique for different periods (from 12 to 72 h) and Ca2+ intracellular fluctuations were evaluated in splenocytes by confocal microscopy. We also performed spleen cell evaluation by flow cytometry and analyzed intracellular Ca2+ mobilization in endoplasmic reticulum and mitochondria. Additionally. Ca2+ channel gene expression was evaluatedResults: Splenocytes showed a progressive loss of intracellular Ca2+ maintenance from endoplasmic reticulum (ER) stores. Transient Ca2+ buffering by the mitochondria was further compromised. These findings were confirmed by changes in mitochondrial integrity and in the performance of the store operated calcium entry (SOCE) and stromal interaction molecule 1 (STIM1) Ca2+ channels.Conclusions and general significance: These novel data suggest that SD impairs Ca2+ signaling, most likely as a result of ER stress, leading to an insufficient Ca2+ supply for signaling events. Our results support the previously described immunosuppressive effects of sleep loss and provide additional information on the cellular and molecular mechanisms involved in sleep function. (C) 2012 Elsevier B.V. All rights reserved. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-12-01 2016-01-24T14:28:02Z 2016-01-24T14:28:02Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbagen.2012.09.010 Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1820, n. 12, p. 1997-2006, 2012. 10.1016/j.bbagen.2012.09.010 WOS000311004000018.pdf 0304-4165 http://repositorio.unifesp.br/handle/11600/35515 WOS:000311004000018 |
| dc.identifier.dark.fl_str_mv |
ark:/48912/001300001vrgb |
| url |
http://dx.doi.org/10.1016/j.bbagen.2012.09.010 http://repositorio.unifesp.br/handle/11600/35515 |
| identifier_str_mv |
Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1820, n. 12, p. 1997-2006, 2012. 10.1016/j.bbagen.2012.09.010 WOS000311004000018.pdf 0304-4165 WOS:000311004000018 ark:/48912/001300001vrgb |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Biochimica Et Biophysica Acta-general Subjects |
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info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
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openAccess |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
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1997-2006 application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier B.V. |
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Elsevier B.V. |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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15,301629 |