Airway obstruction produces widespread sympathoexcitation: role of hypoxia, carotid chemoreceptors, and NTS neurotransmission

Obstructive sleep apnea (OSA) is the most common respiratory disturbance of sleep and is closely associated to cardiovascular diseases. In humans, apnea increases respiratory effort and elevates muscle sympathetic nerve activity (SNA), but the primary stimulus for the SNA activation has not been ide...

Descripción completa

Detalles Bibliográficos
Autores: Ferreira, Caroline B. [UNIFESP], Cravo, Sergio L. [UNIFESP], Stocker, Sean D.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/54198
Acceso en línea:http://dx.doi.org/10.14814/phy2.13536
https://repositorio.unifesp.br/handle/11600/54198
Access Level:acceso abierto
Palabra clave:Apnea
carotid chemoreceptors
NTS
sympathetic nerve activity
id BR_ebb6a33e3bcafa8ac0bbe918267bc4ce
oai_identifier_str oai:repositorio.unifesp.br:11600/54198
network_acronym_str BR
network_name_str Brasil
repository_id_str
spelling Airway obstruction produces widespread sympathoexcitation: role of hypoxia, carotid chemoreceptors, and NTS neurotransmissionApneacarotid chemoreceptorsNTSsympathetic nerve activityObstructive sleep apnea (OSA) is the most common respiratory disturbance of sleep and is closely associated to cardiovascular diseases. In humans, apnea increases respiratory effort and elevates muscle sympathetic nerve activity (SNA), but the primary stimulus for the SNA activation has not been identified. We recently developed a model of apnea in rodents using acute airway obstruction. In this study, we employed this model to test whether the elevation in SNA was mediated by hypoxia, carotid chemoreceptors, or neurotransmission in the nucleus tractus solitarius (NTS). In anesthetized, male Sprague-Dawley rats, airway obstruction (20s) increased phrenic nerve activity (PNA), arterial blood pressure (ABP), and lumbar, renal, and splanchnic SNA. The changes in SNA were similar across all three sympathetic nerves. Inactivation of chemoreceptors by hyperoxia (100% O-2) or surgical denervation of carotid chemoreceptors attenuated, but did not eliminate, the changes in SNA and ABP produced by airway obstruction. To interrupt afferent information from carotid chemoreceptor and extracarotid afferents to the hindbrain, airway obstruction was performed before and after NTS microinjection of the GABA(A) agonist muscimol or a cocktail of NMDA and non-NMDA antagonists. Inhibition of NTS neurons or blockade of glutamatergic receptors attenuated the increase in lumbar SNA, splanchnic SNA, renal SNA, and PNA. Collectively, these findings suggest that PNA and SNA responses induced by airway obstruction depend, in part, on chemoreceptors afferents and glutamatergic neurotransmission in the NTS.Univ Fed Sao Paulo, Dept Physiol, Sao Paulo, SP, BrazilUniv Pittsburgh, Sch Med, Dept Med, Div Renal Electrolyte, Pittsburgh, PA 15213 USAUniv Pittsburgh, Sch Med, Hypertens Ctr, Pittsburgh, PA USAUniv Fed Sao Paulo, Dept Physiol, Sao Paulo, SP, BrazilWeb of ScienceNational Heart, Lung, and Blood Institute GrantAmerican Heart Association Established Investigator AwardCoordination of Training of Higher Education Graduate Foundation (CAPES)National Council for Scientific and Technological Development (CNPq)National Heart, Lung, and Blood Institute Grant: R01 HL113270Wiley2020-07-08T13:09:46Z2020-07-08T13:09:46Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttp://dx.doi.org/10.14814/phy2.13536Physiological Reports. Hoboken, v. 6, n. 3, p. -, 2018.10.14814/phy2.13536WOS000425110700002.pdf2051-817Xhttps://repositorio.unifesp.br/handle/11600/54198WOS:000425110700002ark:/48912/001300001kcz5engPhysiological ReportsHobokeninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPFerreira, Caroline B. [UNIFESP]Cravo, Sergio L. [UNIFESP]Stocker, Sean D.2024-08-03T06:45:22Zoai:repositorio.unifesp.br:11600/54198Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T06:45:22Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Airway obstruction produces widespread sympathoexcitation: role of hypoxia, carotid chemoreceptors, and NTS neurotransmission
title Airway obstruction produces widespread sympathoexcitation: role of hypoxia, carotid chemoreceptors, and NTS neurotransmission
spellingShingle Airway obstruction produces widespread sympathoexcitation: role of hypoxia, carotid chemoreceptors, and NTS neurotransmission
Ferreira, Caroline B. [UNIFESP]
Apnea
carotid chemoreceptors
NTS
sympathetic nerve activity
title_short Airway obstruction produces widespread sympathoexcitation: role of hypoxia, carotid chemoreceptors, and NTS neurotransmission
title_full Airway obstruction produces widespread sympathoexcitation: role of hypoxia, carotid chemoreceptors, and NTS neurotransmission
title_fullStr Airway obstruction produces widespread sympathoexcitation: role of hypoxia, carotid chemoreceptors, and NTS neurotransmission
title_full_unstemmed Airway obstruction produces widespread sympathoexcitation: role of hypoxia, carotid chemoreceptors, and NTS neurotransmission
title_sort Airway obstruction produces widespread sympathoexcitation: role of hypoxia, carotid chemoreceptors, and NTS neurotransmission
dc.creator.none.fl_str_mv Ferreira, Caroline B. [UNIFESP]
Cravo, Sergio L. [UNIFESP]
Stocker, Sean D.
author Ferreira, Caroline B. [UNIFESP]
author_facet Ferreira, Caroline B. [UNIFESP]
Cravo, Sergio L. [UNIFESP]
Stocker, Sean D.
author_role author
author2 Cravo, Sergio L. [UNIFESP]
Stocker, Sean D.
author2_role author
author
dc.subject.por.fl_str_mv Apnea
carotid chemoreceptors
NTS
sympathetic nerve activity
topic Apnea
carotid chemoreceptors
NTS
sympathetic nerve activity
description Obstructive sleep apnea (OSA) is the most common respiratory disturbance of sleep and is closely associated to cardiovascular diseases. In humans, apnea increases respiratory effort and elevates muscle sympathetic nerve activity (SNA), but the primary stimulus for the SNA activation has not been identified. We recently developed a model of apnea in rodents using acute airway obstruction. In this study, we employed this model to test whether the elevation in SNA was mediated by hypoxia, carotid chemoreceptors, or neurotransmission in the nucleus tractus solitarius (NTS). In anesthetized, male Sprague-Dawley rats, airway obstruction (20s) increased phrenic nerve activity (PNA), arterial blood pressure (ABP), and lumbar, renal, and splanchnic SNA. The changes in SNA were similar across all three sympathetic nerves. Inactivation of chemoreceptors by hyperoxia (100% O-2) or surgical denervation of carotid chemoreceptors attenuated, but did not eliminate, the changes in SNA and ABP produced by airway obstruction. To interrupt afferent information from carotid chemoreceptor and extracarotid afferents to the hindbrain, airway obstruction was performed before and after NTS microinjection of the GABA(A) agonist muscimol or a cocktail of NMDA and non-NMDA antagonists. Inhibition of NTS neurons or blockade of glutamatergic receptors attenuated the increase in lumbar SNA, splanchnic SNA, renal SNA, and PNA. Collectively, these findings suggest that PNA and SNA responses induced by airway obstruction depend, in part, on chemoreceptors afferents and glutamatergic neurotransmission in the NTS.
publishDate 2018
dc.date.none.fl_str_mv 2018
2020-07-08T13:09:46Z
2020-07-08T13:09:46Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.14814/phy2.13536
Physiological Reports. Hoboken, v. 6, n. 3, p. -, 2018.
10.14814/phy2.13536
WOS000425110700002.pdf
2051-817X
https://repositorio.unifesp.br/handle/11600/54198
WOS:000425110700002
dc.identifier.dark.fl_str_mv ark:/48912/001300001kcz5
url http://dx.doi.org/10.14814/phy2.13536
https://repositorio.unifesp.br/handle/11600/54198
identifier_str_mv Physiological Reports. Hoboken, v. 6, n. 3, p. -, 2018.
10.14814/phy2.13536
WOS000425110700002.pdf
2051-817X
WOS:000425110700002
ark:/48912/001300001kcz5
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Physiological Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.coverage.none.fl_str_mv Hoboken
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1853670463211831296
score 15,298079

Similares en LA Referencia