Differentially expressed proteins in positive versus negative HNSCC lymph nodes

Background: Lymph node metastasis is one of the most important prognostic factors in head and neck squamous cell carcinomas (HNSCCs) and critical for delineating their treatment. However, clinical and histological criteria for the diagnosis of nodal status remain limited. In the present study, we ai...

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Autores: Vidotto, Alessandra, Polachini, Giovana M., De Paula-Silva, Marina [UNESP], Oliani, Sonia M. [UNESP], Henrique, Tiago, López, Rossana V. M., Cury, Patrícia M., Nunes, Fabio D., Góis-Filho, José F., De Carvalho, Marcos B., Leopoldino, Andréia M., Tajara, Eloiza H.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/176786
Acceso en línea:http://dx.doi.org/10.1186/s12920-018-0382-6
http://hdl.handle.net/11449/176786
Access Level:acceso abierto
Palabra clave:Head and neck carcinoma
Lymph node
Metastasis
Proteomics
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spelling Differentially expressed proteins in positive versus negative HNSCC lymph nodesHead and neck carcinomaLymph nodeMetastasisProteomicsBackground: Lymph node metastasis is one of the most important prognostic factors in head and neck squamous cell carcinomas (HNSCCs) and critical for delineating their treatment. However, clinical and histological criteria for the diagnosis of nodal status remain limited. In the present study, we aimed to characterize the proteomic profile of lymph node metastasis from HNSCC patients. Methods: In the present study, we used one- and two-dimensional electrophoresis and mass spectrometry analysis to characterize the proteomic profile of lymph node metastasis from HNSCC. Results: Comparison of metastatic and non-metastatic lymph nodes showed 52 differentially expressed proteins associated with neoplastic development and progression. The results reinforced the idea that tumors from different anatomical subsites have dissimilar behaviors, which may be influenced by micro-environmental factor including the lymphatic network. The expression pattern of heat shock proteins and glycolytic enzymes also suggested an effect of the lymph node environment in controlling tumor growth or in metabolic reprogramming of the metastatic cell. Our study, for the first time, provided direct evidence of annexin A1 overexpression in lymph node metastasis of head and neck cancer, adding information that may be useful for diagnosing aggressive disease. Conclusions: In brief, this study contributed to our understanding of the metastatic phenotype of HNSCC and provided potential targets for diagnostic in this group of carcinomas.Departamento de Biologia Molecular Faculdade de Medicina (FAMERP), Av. Brigadeiro Faria Lima, 5416, Vila São PedroDepartamento de Biologia Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista (UNESP), R. Cristóvão Colombo, 2265Instituto Do Câncer de São Paulo Octavio Frias de Oliveira - ICESP, Av. Dr. Arnaldo, 251 - Cerqueira CésarFaculdade Ceres (Faceres), Av. Anísio Haddad, 6751Departamento de Estomatologia Faculdade de Odontologia Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227Instituto Do Câncer Arnaldo Vieira de Carvalho, R. Dr Cesário Mota Jr, 112Departamento de Cirurgia de Cabeça e Pescoço Hospital Heliópolis, R. Cônego Xavier, 276Departamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas Universidade de São Paulo, Avenida do Café, s/nDepartamento de Genética e Biologia Evolutiva Instituto de Biociências Universidade de São Paulo, R. do Matão, 321Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista (UNESP), R. Cristóvão Colombo, 2265Faculdade de Medicina (FAMERP)Universidade Estadual Paulista (Unesp)Instituto Do Câncer de São Paulo Octavio Frias de Oliveira - ICESPFaculdade Ceres (Faceres)Universidade de São Paulo (USP)Instituto Do Câncer Arnaldo Vieira de CarvalhoHospital Heliópolis2018-12-11T17:22:29Z2018-12-11T17:22:29Z2018-08-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12920-018-0382-6BMC Medical Genomics, v. 11, n. 1, 2018.1755-8794http://hdl.handle.net/11449/17678610.1186/s12920-018-0382-62-s2.0-850527454062-s2.0-85052745406.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Medical Genomics1,688info:eu-repo/semantics/openAccessVidotto, AlessandraPolachini, Giovana M.De Paula-Silva, Marina [UNESP]Oliani, Sonia M. [UNESP]Henrique, TiagoLópez, Rossana V. M.Cury, Patrícia M.Nunes, Fabio D.Góis-Filho, José F.De Carvalho, Marcos B.Leopoldino, Andréia M.Tajara, Eloiza H.2024-10-25T14:11:18Zoai:repositorio.unesp.br:11449/176786Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-10-25T14:11:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Differentially expressed proteins in positive versus negative HNSCC lymph nodes
title Differentially expressed proteins in positive versus negative HNSCC lymph nodes
spellingShingle Differentially expressed proteins in positive versus negative HNSCC lymph nodes
Vidotto, Alessandra
Head and neck carcinoma
Lymph node
Metastasis
Proteomics
title_short Differentially expressed proteins in positive versus negative HNSCC lymph nodes
title_full Differentially expressed proteins in positive versus negative HNSCC lymph nodes
title_fullStr Differentially expressed proteins in positive versus negative HNSCC lymph nodes
title_full_unstemmed Differentially expressed proteins in positive versus negative HNSCC lymph nodes
title_sort Differentially expressed proteins in positive versus negative HNSCC lymph nodes
dc.creator.none.fl_str_mv Vidotto, Alessandra
Polachini, Giovana M.
De Paula-Silva, Marina [UNESP]
Oliani, Sonia M. [UNESP]
Henrique, Tiago
López, Rossana V. M.
Cury, Patrícia M.
Nunes, Fabio D.
Góis-Filho, José F.
De Carvalho, Marcos B.
Leopoldino, Andréia M.
Tajara, Eloiza H.
author Vidotto, Alessandra
author_facet Vidotto, Alessandra
Polachini, Giovana M.
De Paula-Silva, Marina [UNESP]
Oliani, Sonia M. [UNESP]
Henrique, Tiago
López, Rossana V. M.
Cury, Patrícia M.
Nunes, Fabio D.
Góis-Filho, José F.
De Carvalho, Marcos B.
Leopoldino, Andréia M.
Tajara, Eloiza H.
author_role author
author2 Polachini, Giovana M.
De Paula-Silva, Marina [UNESP]
Oliani, Sonia M. [UNESP]
Henrique, Tiago
López, Rossana V. M.
Cury, Patrícia M.
Nunes, Fabio D.
Góis-Filho, José F.
De Carvalho, Marcos B.
Leopoldino, Andréia M.
Tajara, Eloiza H.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Faculdade de Medicina (FAMERP)
Universidade Estadual Paulista (Unesp)
Instituto Do Câncer de São Paulo Octavio Frias de Oliveira - ICESP
Faculdade Ceres (Faceres)
Universidade de São Paulo (USP)
Instituto Do Câncer Arnaldo Vieira de Carvalho
Hospital Heliópolis
dc.subject.por.fl_str_mv Head and neck carcinoma
Lymph node
Metastasis
Proteomics
topic Head and neck carcinoma
Lymph node
Metastasis
Proteomics
description Background: Lymph node metastasis is one of the most important prognostic factors in head and neck squamous cell carcinomas (HNSCCs) and critical for delineating their treatment. However, clinical and histological criteria for the diagnosis of nodal status remain limited. In the present study, we aimed to characterize the proteomic profile of lymph node metastasis from HNSCC patients. Methods: In the present study, we used one- and two-dimensional electrophoresis and mass spectrometry analysis to characterize the proteomic profile of lymph node metastasis from HNSCC. Results: Comparison of metastatic and non-metastatic lymph nodes showed 52 differentially expressed proteins associated with neoplastic development and progression. The results reinforced the idea that tumors from different anatomical subsites have dissimilar behaviors, which may be influenced by micro-environmental factor including the lymphatic network. The expression pattern of heat shock proteins and glycolytic enzymes also suggested an effect of the lymph node environment in controlling tumor growth or in metabolic reprogramming of the metastatic cell. Our study, for the first time, provided direct evidence of annexin A1 overexpression in lymph node metastasis of head and neck cancer, adding information that may be useful for diagnosing aggressive disease. Conclusions: In brief, this study contributed to our understanding of the metastatic phenotype of HNSCC and provided potential targets for diagnostic in this group of carcinomas.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:22:29Z
2018-12-11T17:22:29Z
2018-08-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s12920-018-0382-6
BMC Medical Genomics, v. 11, n. 1, 2018.
1755-8794
http://hdl.handle.net/11449/176786
10.1186/s12920-018-0382-6
2-s2.0-85052745406
2-s2.0-85052745406.pdf
url http://dx.doi.org/10.1186/s12920-018-0382-6
http://hdl.handle.net/11449/176786
identifier_str_mv BMC Medical Genomics, v. 11, n. 1, 2018.
1755-8794
10.1186/s12920-018-0382-6
2-s2.0-85052745406
2-s2.0-85052745406.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BMC Medical Genomics
1,688
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15.300719

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