Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
Introduction: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. Methods: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to...
| Autores: | , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Brasil |
| Recursos: | Universidade Federal do Pará (UFPA) |
| Repositorio: | Repositório Institucional da UFPA |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.ufpa.br:2011/10032 |
| Acesso em linha: | http://repositorio.ufpa.br/jspui/handle/2011/10032 http://dx.doi.org/10.1590/0037-8682-0220-2017 |
| Access Level: | acceso abierto |
| Palavra-chave: | CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA Vírus da febre amarela Infecção viral Partículas pseudo-infecciosas Flavivírus |
| Resumo: | Introduction: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. Methods: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. Results: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. Conclusions: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application. |
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