Sodium selenite supplementation does not fully restore oxidative stress-induced deiodinase dysfunction : implications for the nonthyroidal illness syndrome
Nonthyroidal illness syndrome (NTIS) is marked by low T3 and high reverse T3 levels. The physio- pathology is poorly understood but involves oxidative stress-induced disruption of the iodothyronine deiodinases, which activate or inactivate thyroid hormones. Selenium, an essential trace element, exer...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Brasil |
| Institución: | Universidade Federal do Rio Grande do Sul (UFRGS) |
| Repositorio: | Repositório Institucional da UFRGS |
| Idioma: | inglés |
| OAI Identifier: | oai:www.lume.ufrgs.br:10183/182273 |
| Acceso en línea: | http://hdl.handle.net/10183/182273 |
| Access Level: | acceso abierto |
| Palabra clave: | Síndromes do eutireóideo doente Espécies reativas de oxigênio Estresse oxidativo Carbonilação proteica Selenito de sódio Deiodinases Sodium selenite Thyroidhormone Nonthyroidalillnesssyndrome |
| Sumario: | Nonthyroidal illness syndrome (NTIS) is marked by low T3 and high reverse T3 levels. The physio- pathology is poorly understood but involves oxidative stress-induced disruption of the iodothyronine deiodinases, which activate or inactivate thyroid hormones. Selenium, an essential trace element, exerts antioxidant function mainly through the thioredoxin reductase (TRx) and glutathione peroxidase (GPx) redox-regulating systems. We evaluated the effect of sodium selenite on IL6-induced disruption on deiodinase function. Cell lines expressing endogenous deiodinases type 1(D1), 2(D2) or 3(D3) (HepG2, MSTO, and MCF-7 cells, respectively) were used in an intact cell model that mimics the deiodination process under physiological conditions of substrate and cofactor, in the presence or not of IL6, with or without selenite. Deiodinase activity was quantified by the amount of iodine-125 in the medium (D1 and D2) or by ion-exchange chromatography (D3). Oxidative stress was evaluated by measuring reactive species (RS), carbonyl content as well as enzymatic and non-enzymatic antioxidant defenses. Results: IL6 induced ROS and carbonyl content in all 3 cell lines (all P o 0.001). Increased ROS was paralleled by D1 and D2-decreased T3-production (P o 0.01) and increased D3-catalyzed T3-inactivation (P o 0.001). Se- lenite decreases the IL6-induced ROS and carbonyl content, while enhances Gpx and Trx activities. Nevertheless, it failed on restoring D1 or D2 function and only attenuates D3 activation (P o 0.05). In conclusion, although sodium selenite reduces IL6-induced redox imbalance it does not fully repair deiodinase function. These results shed light on NTIS physiopathology and might explain why low T3 levels are unaffected by selenium supplementation in sick patients. |
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