Antimicrobial susceptibility of photodynamic therapy (UVA/riboflavin) against Staphylococcus aureus
Purpose: To assess S. aureus in vitro viability after the exposure to ultraviolet light A (UVA) and riboflavin (B2).Methods: Samples of S. aureus in 96 well plates (in triplicate) were exposed to riboflavin (B2) and ultraviolet light A (365 nm wavelength) at a power density of 3 mW/cm(2), 8 mm spot...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | Brasil |
| Institución: | Universidade Federal de São Paulo (UNIFESP) |
| Repositorio: | Repositório Institucional da UNIFESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/42723 |
| Acceso en línea: | http://dx.doi.org/10.1590/S0004-27492012000600011 http://repositorio.unifesp.br/handle/11600/42723 |
| Access Level: | acceso abierto |
| Palabra clave: | Keratitis Riboflavin Corneal ulcer Staphylococcus aureus Ultraviolet rays Cross-linking reagents |
| Sumario: | Purpose: To assess S. aureus in vitro viability after the exposure to ultraviolet light A (UVA) and riboflavin (B2).Methods: Samples of S. aureus in 96 well plates (in triplicate) were exposed to riboflavin (B2) and ultraviolet light A (365 nm wavelength) at a power density of 3 mW/cm(2), 8 mm spot diameter, for 30 minutes. Control groups were prepared as well in triplicate: blank control, ultraviolet light A only, riboflavin only and dead bacteria Control. The bacterial viability was measured using fluorescent microscopy. In order to investigate the occurrence of viable but non-culturable microorganisms after treatment, the cell viability was also investigated by plate culture procedure onto a broth medium. Statistical analysis was performed using the triplicate values from each experimental condition.Results: No difference was observed among the treatment group and the control samples (p=1).Conclusion: The combination of riboflavin 0.1% and ultraviolet light A at 365 nm did not exhibit antimicrobial activity against oxacillin susceptible S. aureus. |
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