Development of a Headspace-Solid Phase Microextraction-Gas Chromatography/Mass Spectrometry (HS-SPME-GC/MS) Method for the Determination of Benzene in Soft Drinks
A method based on headspace-solid phase microextraction and gas chromatography with mass spectrometry has been developed and validated for the determination of benzene in soft drinks. The extraction step was optimized using a rotatable central composite design including the following experimental va...
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Brasil |
| Recursos: | Instituto de Tecnologia de Alimentos (ITAL) |
| Repositorio: | Repositório do Instituto de Tecnologia de Alimentos |
| Idioma: | inglés |
| OAI Identifier: | oai:http://repositorio.ital.sp.gov.br:123456789/407 |
| Acesso em linha: | http://repositorio.ital.sp.gov.br/jspui/handle/123456789/407 |
| Access Level: | acceso abierto |
| Palavra-chave: | Benzene Soft drinks Solid phase microextraction GC/MS |
| Resumo: | A method based on headspace-solid phase microextraction and gas chromatography with mass spectrometry has been developed and validated for the determination of benzene in soft drinks. The extraction step was optimized using a rotatable central composite design including the following experimental variables: extraction temperature, extraction time, sample weight, and salt concentration. The optimized procedure, which was carried out at 30 °C during 30 min by using a 75 μm carboxen-polydimethylsiloxane fiber, showed good linearity within the concentration range 0–25 μgkg−1 (r2>0.999), mean recoveries from 97.5 to 103.1 %, and coefficients of variation from 1.5 to 13.4 % for repeatability and from 1.5 to 15.7 % for withinlaboratory reproducibility. Limits of detection and quantification were calculated at 0.02 and 0.08 μgkg−1, respectively. The method was applied to determine the concentrations of benzene in 77 samples of beverages from the Brazilian market. Levels from <0.08 to 10.84 μgkg−1 were obtained, which are comparable to those verified in other countries. Most of the samples (72.2 %) contained benzene up to 1 μgkg−1. |
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