Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR

Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.

Detalhes bibliográficos
Autores: Abreu, Hellen, Royer, Carla Adriane, Poitevin, Carolina Gracia, Kohler, Ana Flávia, Rodrigues, Ana Carolina, Raboni, Sonia Mara, Nogueira, Meri Bordignon, Cardoso, Pedro Henrique, Arruda, Mônica Barcellos, Baptista, Patrícia Alvarez da Silva, Bonatto, Ana Claudia, Gradia, Daniela Fiori, Adamoski, Douglas, Souza, Emanuel Maltempi de, Oliveira, Jaqueline Carvalho de
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Brasil
Recursos:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/67195
Acesso em linha:https://arca.fiocruz.br/handle/icict/67195
Access Level:acceso abierto
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dc.title.none.fl_str_mv Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR
title Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR
spellingShingle Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR
Abreu, Hellen
title_short Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR
title_full Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR
title_fullStr Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR
title_full_unstemmed Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR
title_sort Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR
dc.creator.none.fl_str_mv Abreu, Hellen
Royer, Carla Adriane
Poitevin, Carolina Gracia
Kohler, Ana Flávia
Rodrigues, Ana Carolina
Raboni, Sonia Mara
Nogueira, Meri Bordignon
Cardoso, Pedro Henrique
Arruda, Mônica Barcellos
Baptista, Patrícia Alvarez da Silva
Bonatto, Ana Claudia
Gradia, Daniela Fiori
Adamoski, Douglas
Souza, Emanuel Maltempi de
Oliveira, Jaqueline Carvalho de
author Abreu, Hellen
author_facet Abreu, Hellen
Royer, Carla Adriane
Poitevin, Carolina Gracia
Kohler, Ana Flávia
Rodrigues, Ana Carolina
Raboni, Sonia Mara
Nogueira, Meri Bordignon
Cardoso, Pedro Henrique
Arruda, Mônica Barcellos
Baptista, Patrícia Alvarez da Silva
Bonatto, Ana Claudia
Gradia, Daniela Fiori
Adamoski, Douglas
Souza, Emanuel Maltempi de
Oliveira, Jaqueline Carvalho de
author_role author
author2 Royer, Carla Adriane
Poitevin, Carolina Gracia
Kohler, Ana Flávia
Rodrigues, Ana Carolina
Raboni, Sonia Mara
Nogueira, Meri Bordignon
Cardoso, Pedro Henrique
Arruda, Mônica Barcellos
Baptista, Patrícia Alvarez da Silva
Bonatto, Ana Claudia
Gradia, Daniela Fiori
Adamoski, Douglas
Souza, Emanuel Maltempi de
Oliveira, Jaqueline Carvalho de
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
description Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.
publishDate 2023
dc.date.issued.fl_str_mv 2023
dc.date.accessioned.fl_str_mv 2024-11-21T18:52:39Z
dc.date.available.fl_str_mv 2024-11-21T18:52:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ABREU, Hellen et al. Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR. Viruses, v. 15, n. 12, p. 1-10, 2024.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/67195
dc.identifier.issn.none.fl_str_mv 1999-4915
dc.identifier.doi.none.fl_str_mv 10.3390/v15122352
identifier_str_mv ABREU, Hellen et al. Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR. Viruses, v. 15, n. 12, p. 1-10, 2024.
1999-4915
10.3390/v15122352
url https://arca.fiocruz.br/handle/icict/67195
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2024-11-21T18:52:39Z2024-11-21T18:52:39Z2023ABREU, Hellen et al. Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR. Viruses, v. 15, n. 12, p. 1-10, 2024.1999-4915https://arca.fiocruz.br/handle/icict/6719510.3390/v15122352engMDPIInfluenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCRinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Laboratório de Pesquisa em Virologia e Biologia Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Laboratório de Pesquisa em Virologia e Biologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Centro Brasileiro de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil.Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.After the Coronavirus pandemic, the importance of virus surveillance was highlighted, reinforcing the constant necessity of discussing and updating the methods for collection and diagnoses, including for other respiratory viruses. Although the nasopharyngeal swab is the gold-standard sample for detecting and genotyping SARS-CoV-2 and Influenza viruses, its collection is uncomfortable and requires specialized teams, which can be costly. During the pandemic, non invasive saliva samples proved to be a suitable alternative for SARS-CoV-2 diagnosis, but for Influenza virus the use of this sample source is not recognized yet. In addition, most SARS-CoV-2 comparisons were conducted before the Omicron variant emerged. Here, we aimed to compare Influenza A and Omicron RT-qPCR analysis of nasopharyngeal swabs and saliva self-collection in paired samples from 663 individuals. We found that both nasopharyngeal swab and saliva collection are efficient for the diagnosis of Omicron (including sub-lineages) and for Influenza A, with high sensitivity and accuracy (>90%). The kappa index is 0.938 for Influenza A and 0.905 for SARS-CoV-2. These results showed excellent agreement between the two samples reinforcing saliva samples as a reliable source for detecting Omicron and highlighting saliva as a valid sample source for Influenza detection, considering this cheaper and more comfortable alternative.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZAbreu, HellenRoyer, Carla AdrianePoitevin, Carolina GraciaKohler, Ana FláviaRodrigues, Ana CarolinaRaboni, Sonia MaraNogueira, Meri BordignonCardoso, Pedro HenriqueArruda, Mônica BarcellosBaptista, Patrícia Alvarez da SilvaBonatto, Ana ClaudiaGradia, Daniela FioriAdamoski, DouglasSouza, Emanuel Maltempi deOliveira, Jaqueline Carvalho deORIGINALve_Abreu_Hellen_etal_biomanguinhos_2024.pdfve_Abreu_Hellen_etal_biomanguinhos_2024.pdfapplication/pdf1779211https://arca.fiocruz.br/bitstreams/32334925-02f5-49e8-ab21-8c7db1ff0f9d/download5682c00988c1b92f84c321606fadf061MD52trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; 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