An L,L- diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis

Lysine (Lys) connects the mitochondrial electron transport chain to amino acid catabolism and the tricarboxylic acid cycle. However, our understanding of how a deficiency in Lys biosynthesis impacts plant metabolism and growth remains limited. Here, we used a previously characterized Arabidopsis mut...

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Autores: Cavalcanti, João Henrique F., Kirma, Menny, Barros, Jessica A. S., Quinhones, Carla G.S., Pereira- Lima, Ítalo A., Obata, Toshihiro, Nunes- Nesi, Adriano, Galili, Gad, Fernie, Alisdair R., Avin- Wittenberg, Tamar, Araújo, Wagner L.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Institución:Universidade Federal de Viçosa (UFV)
Repositorio:LOCUS Repositório Institucional da UFV
Idioma:inglés
OAI Identifier:oai:locus.ufv.br:123456789/24551
Acceso en línea:http://dx.doi.org/10.1093/jxb/ery325
http://www.locus.ufv.br/handle/123456789/24551
Access Level:acceso abierto
Palabra clave:Alternative respiration
Amino acids
Carbon partition
L, L- diaminopimelate aminotransferase
Lysine biosynthesis
Primary metabolism
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spelling An L,L- diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in ArabidopsisAlternative respirationAmino acidsCarbon partitionL, L- diaminopimelate aminotransferaseLysine biosynthesisPrimary metabolismLysine (Lys) connects the mitochondrial electron transport chain to amino acid catabolism and the tricarboxylic acid cycle. However, our understanding of how a deficiency in Lys biosynthesis impacts plant metabolism and growth remains limited. Here, we used a previously characterized Arabidopsis mutant (dapat) with reduced activity of the Lys biosynthesis enzyme L,L-diaminopimelate aminotransferase to investigate the physiological and metabolic impacts of impaired Lys biosynthesis. Despite displaying similar stomatal conductance and internal CO2 concentration, we observed reduced photosynthesis and growth in the dapat mutant. Surprisingly, whilst we did not find differences in dark respiration between genotypes, a lower storage and consumption of starch and sugars was observed in dapat plants. We found higher protein turnover but no differences in total amino acids during a diurnal cycle in dapat plants. Transcriptional and two-dimensional (isoelectric focalization/SDS-PAGE) proteome analyses revealed alterations in the abundance of several transcripts and proteins associated with photosynthesis and photorespiration coupled with a high glycine/serine ratio and increased levels of stress-responsive amino acids. Taken together, our findings demonstrate that biochemical alterations rather than stomatal limitations are responsible for the decreased photosynthesis and growth of the dapat mutant, which we hypothesize mimics stress conditions associated with impairments in the Lys biosynthesis pathway.Journal of Experimental Botany2019-04-15T14:14:02Z2019-04-15T14:14:02Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1460-2431http://dx.doi.org/10.1093/jxb/ery325http://www.locus.ufv.br/handle/123456789/24551engVolume 69, Issue 22, Pages 5489– 5506, 1 December 2018info:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVCavalcanti, João Henrique F.Kirma, MennyBarros, Jessica A. S.Quinhones, Carla G.S.Pereira- Lima, Ítalo A.Obata, ToshihiroNunes- Nesi, AdrianoGalili, GadFernie, Alisdair R.Avin- Wittenberg, TamarAraújo, Wagner L.2024-07-12T07:54:48Zoai:locus.ufv.br:123456789/24551Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:54:48LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv An L,L- diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis
title An L,L- diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis
spellingShingle An L,L- diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis
Cavalcanti, João Henrique F.
Alternative respiration
Amino acids
Carbon partition
L, L- diaminopimelate aminotransferase
Lysine biosynthesis
Primary metabolism
title_short An L,L- diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis
title_full An L,L- diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis
title_fullStr An L,L- diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis
title_full_unstemmed An L,L- diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis
title_sort An L,L- diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis
dc.creator.none.fl_str_mv Cavalcanti, João Henrique F.
Kirma, Menny
Barros, Jessica A. S.
Quinhones, Carla G.S.
Pereira- Lima, Ítalo A.
Obata, Toshihiro
Nunes- Nesi, Adriano
Galili, Gad
Fernie, Alisdair R.
Avin- Wittenberg, Tamar
Araújo, Wagner L.
author Cavalcanti, João Henrique F.
author_facet Cavalcanti, João Henrique F.
Kirma, Menny
Barros, Jessica A. S.
Quinhones, Carla G.S.
Pereira- Lima, Ítalo A.
Obata, Toshihiro
Nunes- Nesi, Adriano
Galili, Gad
Fernie, Alisdair R.
Avin- Wittenberg, Tamar
Araújo, Wagner L.
author_role author
author2 Kirma, Menny
Barros, Jessica A. S.
Quinhones, Carla G.S.
Pereira- Lima, Ítalo A.
Obata, Toshihiro
Nunes- Nesi, Adriano
Galili, Gad
Fernie, Alisdair R.
Avin- Wittenberg, Tamar
Araújo, Wagner L.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Alternative respiration
Amino acids
Carbon partition
L, L- diaminopimelate aminotransferase
Lysine biosynthesis
Primary metabolism
topic Alternative respiration
Amino acids
Carbon partition
L, L- diaminopimelate aminotransferase
Lysine biosynthesis
Primary metabolism
description Lysine (Lys) connects the mitochondrial electron transport chain to amino acid catabolism and the tricarboxylic acid cycle. However, our understanding of how a deficiency in Lys biosynthesis impacts plant metabolism and growth remains limited. Here, we used a previously characterized Arabidopsis mutant (dapat) with reduced activity of the Lys biosynthesis enzyme L,L-diaminopimelate aminotransferase to investigate the physiological and metabolic impacts of impaired Lys biosynthesis. Despite displaying similar stomatal conductance and internal CO2 concentration, we observed reduced photosynthesis and growth in the dapat mutant. Surprisingly, whilst we did not find differences in dark respiration between genotypes, a lower storage and consumption of starch and sugars was observed in dapat plants. We found higher protein turnover but no differences in total amino acids during a diurnal cycle in dapat plants. Transcriptional and two-dimensional (isoelectric focalization/SDS-PAGE) proteome analyses revealed alterations in the abundance of several transcripts and proteins associated with photosynthesis and photorespiration coupled with a high glycine/serine ratio and increased levels of stress-responsive amino acids. Taken together, our findings demonstrate that biochemical alterations rather than stomatal limitations are responsible for the decreased photosynthesis and growth of the dapat mutant, which we hypothesize mimics stress conditions associated with impairments in the Lys biosynthesis pathway.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
2019-04-15T14:14:02Z
2019-04-15T14:14:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1460-2431
http://dx.doi.org/10.1093/jxb/ery325
http://www.locus.ufv.br/handle/123456789/24551
identifier_str_mv 1460-2431
url http://dx.doi.org/10.1093/jxb/ery325
http://www.locus.ufv.br/handle/123456789/24551
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Volume 69, Issue 22, Pages 5489– 5506, 1 December 2018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Journal of Experimental Botany
publisher.none.fl_str_mv Journal of Experimental Botany
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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score 15,300724

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