Enzymatic hydrolysis of Moringa oleifera Lam flour using bromelain and fig by-products as sources of protease

Moringa oleifera Lamarck flour (MOF) has been recognized as a source of vegetable proteins; however, around 70.1±0.98% of protein can be called “insoluble protein”, for its resistance to gastrointestinal digestion. For protein to be absorbed in the human intestinal tract, it must be consumed in the...

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Bibliographic Details
Authors: Bustamante, Lady Johana Arismendi [UNESP], Maisa, Davanso [UNESP], de Cerqueira e Silva, Mariana Barros, de Paula, Ariela Veloso [UNESP], Teixeira, Estelamar Maria Borges, Bassan, Juliana Cristina [UNESP]
Format: article
Status:Published version
Publication Date:2022
Country:Brasil
Institution:Universidade Estadual Paulista (UNESP)
Repository:Repositório Institucional da UNESP
Language:English
OAI Identifier:oai:repositorio.unesp.br:11449/248391
Online Access:http://dx.doi.org/10.1016/j.focha.2022.100133
http://hdl.handle.net/11449/248391
Access Level:Open access
Keyword:By-product
Enzymatic hydrolysis
Fig latex
Moringa oleifera leaves
Pineapple crown
Protein
Description
Summary:Moringa oleifera Lamarck flour (MOF) has been recognized as a source of vegetable proteins; however, around 70.1±0.98% of protein can be called “insoluble protein”, for its resistance to gastrointestinal digestion. For protein to be absorbed in the human intestinal tract, it must be consumed in the form of a soluble protein. Proteases have shown to increase soluble proteins. The objective of this study was, hence, to investigate the increase in soluble proteins of MOF soluble and insoluble water fraction through the hydrolysis with proteases obtained from fig and pineapple by-products, ficin and bromelain, respectively. In conclusion, the protein of MOF had changes in their solubility by enzymatic effect. In particular, the hydrolysis of water insoluble Moringa oleifera Lam flour fraction (IMOF) with ficin contributed to a significant increase in soluble proteins, a result detectable through the different methods applied (colorimetric Bradford method, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and Reversed phase high-performance liquid chromatography (RP-HPLC)).

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