The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses

University of New South Wales. School of Biotechnology and Biomolecular Sciences. Sydney, Australia.

Detalhes bibliográficos
Autores: Tuipulotu, Daniel Enosi, Fumian, Tulio M., Netzler, Natalie E., Mackenzie, Jason M., White, Peter A.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Recursos:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/35506
Acesso em linha:https://arca.fiocruz.br/handle/icict/35506
Access Level:acceso abierto
Palavra-chave:Norovirus
Caliciviridae
Antivirais
Nucleosídeos
Inibidor da polimerase
Antivirals
Polymerase inhibitor
Nucleoside analogue
Caliciviruses
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dc.title.none.fl_str_mv The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses
title The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses
spellingShingle The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses
Tuipulotu, Daniel Enosi
Norovirus
Caliciviridae
Antivirais
Nucleosídeos
Inibidor da polimerase
Norovirus
Antivirals
Polymerase inhibitor
Nucleoside analogue
Caliciviruses
Nucleosídeos
title_short The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses
title_full The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses
title_fullStr The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses
title_full_unstemmed The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses
title_sort The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses
dc.creator.none.fl_str_mv Tuipulotu, Daniel Enosi
Fumian, Tulio M.
Netzler, Natalie E.
Mackenzie, Jason M.
White, Peter A.
author Tuipulotu, Daniel Enosi
author_facet Tuipulotu, Daniel Enosi
Fumian, Tulio M.
Netzler, Natalie E.
Mackenzie, Jason M.
White, Peter A.
author_role author
author2 Fumian, Tulio M.
Netzler, Natalie E.
Mackenzie, Jason M.
White, Peter A.
author2_role author
author
author
author
dc.subject.other.none.fl_str_mv Norovirus
Caliciviridae
Antivirais
Nucleosídeos
Inibidor da polimerase
topic Norovirus
Caliciviridae
Antivirais
Nucleosídeos
Inibidor da polimerase
Norovirus
Antivirals
Polymerase inhibitor
Nucleoside analogue
Caliciviruses
Nucleosídeos
dc.subject.en.none.fl_str_mv Norovirus
Antivirals
Polymerase inhibitor
Nucleoside analogue
Caliciviruses
dc.subject.decs.none.fl_str_mv Nucleosídeos
description University of New South Wales. School of Biotechnology and Biomolecular Sciences. Sydney, Australia.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-12T10:50:31Z
dc.date.available.fl_str_mv 2019-09-12T10:50:31Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TUIPULOTU, Daniel Elosi et al. The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses. Viruses, v. 11, n. 496, p. 1-12, May 2019.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/35506
dc.identifier.issn.none.fl_str_mv 1999-4915
dc.identifier.doi.none.fl_str_mv 10.3390/v11060496
dc.identifier.eissn.none.fl_str_mv 1999-4915
identifier_str_mv TUIPULOTU, Daniel Elosi et al. The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses. Viruses, v. 11, n. 496, p. 1-12, May 2019.
1999-4915
10.3390/v11060496
url https://arca.fiocruz.br/handle/icict/35506
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2019-09-12T10:50:31Z2019-09-12T10:50:31Z2019TUIPULOTU, Daniel Elosi et al. The Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Caliciviruses. Viruses, v. 11, n. 496, p. 1-12, May 2019.1999-4915https://arca.fiocruz.br/handle/icict/3550610.3390/v110604961999-4915engMDPINorovirusCaliciviridaeAntiviraisNucleosídeosInibidor da polimeraseNorovirusAntiviralsPolymerase inhibitorNucleoside analogueCalicivirusesNucleosídeosThe Adenosine Analogue NITD008 has Potent Antiviral Activity against Human and Animal Calicivirusesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of New South Wales. School of Biotechnology and Biomolecular Sciences. Sydney, Australia.University of New South Wales. School of Biotechnology and Biomolecular Sciences. Sydney, Australia / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.University of New South Wales. School of Biotechnology and Biomolecular Sciences. Sydney, Australia.University of Melbourne. Peter Doherty Institute for Infection and Immunity. Department of Microbiology and Immunology. Melbourne, Australia.University of New South Wales. School of Biotechnology and Biomolecular Sciences. Sydney, Australia.The widespread nature of calicivirus infections globally has a substantial impact on the health and well-being of humans and animals alike. Currently, the only vaccines approved against caliciviruses are for feline and rabbit-specific members of this group, and thus there is a growing effort towards the development of broad-spectrum antivirals for calicivirus infections. In this study, we evaluated the antiviral activity of the adenosine analogue NITD008 in vitro using three calicivirus model systems namely; feline calicivirus (FCV), murine norovirus (MNV), and the human norovirus replicon. We show that the nucleoside analogue (NA), NITD008, has limited toxicity and inhibits calicivirus replication in all three model systems with EC50 values of 0.94 μM, 0.91 µM, and 0.21 µM for MNV, FCV, and the Norwalk replicon, respectively. NITD008 has a similar level of potency to the most well-studied NA 2'-C-methylcytidine in vitro. Significantly, we also show that continual NITD008 treatment effectively cleared the Norwalk replicon from cells and treatment with 5 µM NITD008 was sufficient to completely prevent rebound. Given the potency displayed by NITD008 against several caliciviruses, we propose that this compound should be interrogated further to assess its effectiveness in vivo. In summary, we have added a potent NA to the current suite of antiviral compounds and provide a NA scaffold that could be further modified for therapeutic use against calicivirus infections.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTuipulotu, Daniel EnosiFumian, Tulio M.Netzler, Natalie E.Mackenzie, Jason M.White, Peter A.LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/56fe934c-0262-4b12-ab9b-1bcca8a8af89/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALTulioMFumian_Natalie Netzler_etal_IOC_2019.pdfTulioMFumian_Natalie Netzler_etal_IOC_2019.pdfapplication/pdf1563641https://arca.fiocruz.br/bitstreams/54624c72-8401-4af8-90c7-1866af7f7a6a/download7ea820d9a23e86c83960308ef1744291MD52trueAnonymousREADTEXTTulioMFumian_Natalie Netzler_etal_IOC_2019.pdf.txtTulioMFumian_Natalie Netzler_etal_IOC_2019.pdf.txtExtracted texttext/plain55595https://arca.fiocruz.br/bitstreams/11eb5451-fb4a-4b50-b075-ae7eb2397620/download89336d2d826a433062657999c70835aeMD59falseAnonymousREADTHUMBNAILTulioMFumian_Natalie Netzler_etal_IOC_2019.pdf.jpgTulioMFumian_Natalie Netzler_etal_IOC_2019.pdf.jpgGenerated Thumbnailimage/jpeg39727https://arca.fiocruz.br/bitstreams/e789b81c-076f-4ece-94bf-23486199630d/download1ffc2c9c1eb0f8f3c188fc9aa4990c6fMD510falseAnonymousREADicict/355062025-12-10 15:58:50.215open.accessoai:arca.fiocruz.br:icict/35506https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T18:58:50Repositório Institucional da FIOCRUZ (ARCA) - 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