Fibrin biopolymer as scaffold candidate to treat bone defects in rats

Background: Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery prop...

ver descrição completa

Detalhes bibliográficos
Autores: Cassaro, Claudia Vilalva [UNESP], Justulin, Luis Antonio [UNESP], De Lima, Patrícia Rodrigues [UNESP], De Assis Golim, Marjorie [UNESP], Biscola, Natália Perussi [UNESP], De Castro, Mateus Vidigal, De Oliveira, Alexandre Leite Rodrigues, Doiche, Danuta Pulz [UNESP], Pereira, Elenize Jamas [UNESP], Ferreira, Rui Seabra [UNESP], Barraviera, Benedito [UNESP]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/199709
Acesso em linha:http://dx.doi.org/10.1590/1678-9199-jvatitd-2019-0027
http://hdl.handle.net/11449/199709
Access Level:acceso abierto
Palavra-chave:Biomaterials
Biphasic calcium
Bone regeneration
Fibrin biopolymer
Fibrin sealant
Mesenchymal stem cells
Phosphate
id BR_e2133ebde7dd5aba0cdc41bfde06ba8b
oai_identifier_str oai:repositorio.unesp.br:11449/199709
network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.none.fl_str_mv Fibrin biopolymer as scaffold candidate to treat bone defects in rats
title Fibrin biopolymer as scaffold candidate to treat bone defects in rats
spellingShingle Fibrin biopolymer as scaffold candidate to treat bone defects in rats
Cassaro, Claudia Vilalva [UNESP]
Biomaterials
Biphasic calcium
Bone regeneration
Fibrin biopolymer
Fibrin sealant
Mesenchymal stem cells
Phosphate
title_short Fibrin biopolymer as scaffold candidate to treat bone defects in rats
title_full Fibrin biopolymer as scaffold candidate to treat bone defects in rats
title_fullStr Fibrin biopolymer as scaffold candidate to treat bone defects in rats
title_full_unstemmed Fibrin biopolymer as scaffold candidate to treat bone defects in rats
title_sort Fibrin biopolymer as scaffold candidate to treat bone defects in rats
dc.creator.none.fl_str_mv Cassaro, Claudia Vilalva [UNESP]
Justulin, Luis Antonio [UNESP]
De Lima, Patrícia Rodrigues [UNESP]
De Assis Golim, Marjorie [UNESP]
Biscola, Natália Perussi [UNESP]
De Castro, Mateus Vidigal
De Oliveira, Alexandre Leite Rodrigues
Doiche, Danuta Pulz [UNESP]
Pereira, Elenize Jamas [UNESP]
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
author Cassaro, Claudia Vilalva [UNESP]
author_facet Cassaro, Claudia Vilalva [UNESP]
Justulin, Luis Antonio [UNESP]
De Lima, Patrícia Rodrigues [UNESP]
De Assis Golim, Marjorie [UNESP]
Biscola, Natália Perussi [UNESP]
De Castro, Mateus Vidigal
De Oliveira, Alexandre Leite Rodrigues
Doiche, Danuta Pulz [UNESP]
Pereira, Elenize Jamas [UNESP]
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
author_role author
author2 Justulin, Luis Antonio [UNESP]
De Lima, Patrícia Rodrigues [UNESP]
De Assis Golim, Marjorie [UNESP]
Biscola, Natália Perussi [UNESP]
De Castro, Mateus Vidigal
De Oliveira, Alexandre Leite Rodrigues
Doiche, Danuta Pulz [UNESP]
Pereira, Elenize Jamas [UNESP]
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
dc.subject.por.fl_str_mv Biomaterials
Biphasic calcium
Bone regeneration
Fibrin biopolymer
Fibrin sealant
Mesenchymal stem cells
Phosphate
topic Biomaterials
Biphasic calcium
Bone regeneration
Fibrin biopolymer
Fibrin sealant
Mesenchymal stem cells
Phosphate
description Background: Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery properties. The regenerative potential of an association of FBP, biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSCs) was evaluated in defects of rat femurs. Methods: Adult male Wistar rats were submitted to a 5-mm defect in the femur. This was filled with the following materials and/or associations: BPC; FBP and BCP; FBP and MSCs; and BCP, FBP and MSCs. Bone defect without filling was defined as the control group. Thirty and sixty days after the procedure, animals were euthanatized and subjected to computed tomography, scanning electron microscopy and qualitative and quantitative histological analysis. Results: It was shown that FBP is a suitable scaffold for bone defects due to the formation of a stable clot that facilitates the handling and optimizes the surgical procedures, allowing also cell adhesion and proliferation. The association between the materials was biocompatible. Progressive deposition of bone matrix was higher in the group treated with FBP and MSCs. Differentiation of mesenchymal stem cells into osteogenic lineage was not necessary to stimulate bone formation. Conclusions: FBP proved to be an excellent scaffold candidate for bone repair therapies due to application ease and biocompatibility with synthetic calcium-based materials. The satisfactory results obtained by the association of FBP with MSCs may provide a more effective and less costly new approach for bone tissue engineering.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
2020-12-12T01:47:11Z
2020-12-12T01:47:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-9199-jvatitd-2019-0027
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 25.
1678-9199
1678-9180
http://hdl.handle.net/11449/199709
10.1590/1678-9199-jvatitd-2019-0027
S1678-91992019000100320
2-s2.0-85075516328
S1678-91992019000100320.pdf
url http://dx.doi.org/10.1590/1678-9199-jvatitd-2019-0027
http://hdl.handle.net/11449/199709
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 25.
1678-9199
1678-9180
10.1590/1678-9199-jvatitd-2019-0027
S1678-91992019000100320
2-s2.0-85075516328
S1678-91992019000100320.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1853672503302422528
spelling Fibrin biopolymer as scaffold candidate to treat bone defects in ratsBiomaterialsBiphasic calciumBone regenerationFibrin biopolymerFibrin sealantMesenchymal stem cellsPhosphateBackground: Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery properties. The regenerative potential of an association of FBP, biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSCs) was evaluated in defects of rat femurs. Methods: Adult male Wistar rats were submitted to a 5-mm defect in the femur. This was filled with the following materials and/or associations: BPC; FBP and BCP; FBP and MSCs; and BCP, FBP and MSCs. Bone defect without filling was defined as the control group. Thirty and sixty days after the procedure, animals were euthanatized and subjected to computed tomography, scanning electron microscopy and qualitative and quantitative histological analysis. Results: It was shown that FBP is a suitable scaffold for bone defects due to the formation of a stable clot that facilitates the handling and optimizes the surgical procedures, allowing also cell adhesion and proliferation. The association between the materials was biocompatible. Progressive deposition of bone matrix was higher in the group treated with FBP and MSCs. Differentiation of mesenchymal stem cells into osteogenic lineage was not necessary to stimulate bone formation. Conclusions: FBP proved to be an excellent scaffold candidate for bone repair therapies due to application ease and biocompatibility with synthetic calcium-based materials. The satisfactory results obtained by the association of FBP with MSCs may provide a more effective and less costly new approach for bone tissue engineering.Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Botucatu Medical School (FMB) São Paulo State University (UNESP)Extracellular Matrix Laboratory Botucatu Biosciences Institute (IBB) São Paulo State University (UNESP)Flow Cytometry Laboratory Blood Center Botucatu Medical School (FMB) São Paulo State University (UNESP)Department of Structural and Functional Biology Biosciences Institute (IB) University of Campinas (UNICAMP)Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Husbandry São Paulo State University (UNESP)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Botucatu Medical School (FMB) São Paulo State University (UNESP)Extracellular Matrix Laboratory Botucatu Biosciences Institute (IBB) São Paulo State University (UNESP)Flow Cytometry Laboratory Blood Center Botucatu Medical School (FMB) São Paulo State University (UNESP)Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Husbandry São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)2020-12-12T01:47:11Z2020-12-12T01:47:11Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1678-9199-jvatitd-2019-0027Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 25.1678-91991678-9180http://hdl.handle.net/11449/19970910.1590/1678-9199-jvatitd-2019-0027S1678-919920190001003202-s2.0-85075516328S1678-91992019000100320.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccessCassaro, Claudia Vilalva [UNESP]Justulin, Luis Antonio [UNESP]De Lima, Patrícia Rodrigues [UNESP]De Assis Golim, Marjorie [UNESP]Biscola, Natália Perussi [UNESP]De Castro, Mateus VidigalDe Oliveira, Alexandre Leite RodriguesDoiche, Danuta Pulz [UNESP]Pereira, Elenize Jamas [UNESP]Ferreira, Rui Seabra [UNESP]Barraviera, Benedito [UNESP]2024-04-11T15:28:35Zoai:repositorio.unesp.br:11449/199709Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-04-11T15:28:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
score 15,301603

Registos relacionados