HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.

Oliveira, Liliam Teixeira; Garcia, Giani Martins; Kano, Eunice Kazue; Tedesco, Antônio Cláudio; Mosqueira, Vanessa Carla Furtado

HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.

Analytical and bioanalytical methods of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were developed and validated for the determination of chloroaluminum phthalocyanine in different formulations of polymeric nanocapsules, plasma and livers of mice. Plasma and homogen...

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Autores:	Oliveira, Liliam Teixeira, Garcia, Giani Martins, Kano, Eunice Kazue, Tedesco, Antônio Cláudio, Mosqueira, Vanessa Carla Furtado
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2011
País:	Brasil
Institución:	Universidade Federal de Ouro Preto (UFOP)
Repositorio:	Repositório Institucional da UFOP
Idioma:	inglés
OAI Identifier:	oai:repositorio.ufop.br:123456789/3770
Acceso en línea:	http://www.repositorio.ufop.br/handle/123456789/3770 https://doi.org/10.1016/j.jpba.2011.04.016
Access Level:	acceso abierto
Palabra clave:	Chloroaluminium phthalocyanine High-performance liquid Nanocapsules Photodynamic therapy Fluorescent dye

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spelling	HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.Chloroaluminium phthalocyanineHigh-performance liquidNanocapsulesPhotodynamic therapyFluorescent dyeAnalytical and bioanalytical methods of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were developed and validated for the determination of chloroaluminum phthalocyanine in different formulations of polymeric nanocapsules, plasma and livers of mice. Plasma and homogenized liver samples were extracted with ethyl acetate, and zinc phthalocyanine was used as internal standard. The results indicated that the methods were linear and selective for all matrices studied. Analysis of accuracy and precision showed adequate values, with variations lower than 10% in biological samples and lower than 2% in analytical samples. The recoveries were as high as 96% and 99% in the plasma and livers, respectively. The quantification limit of the analytical method was 1.12 ng/ml, and the limits of quantification of the bioanalytical method were 15 ng/ml and 75 ng/g for plasma and liver samples, respectively. The bioanalytical method developed was sensitive in the ranges of 15–100 ng/ml in plasma and 75–500 ng/g in liver samples and was applied to studies of biodistribution and pharmacokinetics of AlClPc.2014-11-11T18:14:12Z2014-11-11T18:14:12Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLIVEIRA, L. M. et al. HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. Journal of Pharmaceutical and Biomedical Analysis. V. 56, p. 70-77, 2011. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0731708511002275>. Acesso em: 20 ago. 2014.0731-7085http://www.repositorio.ufop.br/handle/123456789/3770https://doi.org/10.1016/j.jpba.2011.04.016ark:/61566/0013000003cknO periódico Journal of Pharmaceutical and Biomedical Analysis concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3457171409676.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPOliveira, Liliam TeixeiraGarcia, Giani MartinsKano, Eunice KazueTedesco, Antônio CláudioMosqueira, Vanessa Carla Furtado2024-11-10T15:08:57Zoai:repositorio.ufop.br:123456789/3770Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332024-11-10T15:08:57Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv	HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.
title	HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.
spellingShingle	HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. Oliveira, Liliam Teixeira Chloroaluminium phthalocyanine High-performance liquid Nanocapsules Photodynamic therapy Fluorescent dye
title_short	HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.
title_full	HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.
title_fullStr	HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.
title_full_unstemmed	HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.
title_sort	HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.
dc.creator.none.fl_str_mv	Oliveira, Liliam Teixeira Garcia, Giani Martins Kano, Eunice Kazue Tedesco, Antônio Cláudio Mosqueira, Vanessa Carla Furtado
author	Oliveira, Liliam Teixeira
author_facet	Oliveira, Liliam Teixeira Garcia, Giani Martins Kano, Eunice Kazue Tedesco, Antônio Cláudio Mosqueira, Vanessa Carla Furtado
author_role	author
author2	Garcia, Giani Martins Kano, Eunice Kazue Tedesco, Antônio Cláudio Mosqueira, Vanessa Carla Furtado
author2_role	author author author author
dc.subject.por.fl_str_mv	Chloroaluminium phthalocyanine High-performance liquid Nanocapsules Photodynamic therapy Fluorescent dye
topic	Chloroaluminium phthalocyanine High-performance liquid Nanocapsules Photodynamic therapy Fluorescent dye
description	Analytical and bioanalytical methods of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were developed and validated for the determination of chloroaluminum phthalocyanine in different formulations of polymeric nanocapsules, plasma and livers of mice. Plasma and homogenized liver samples were extracted with ethyl acetate, and zinc phthalocyanine was used as internal standard. The results indicated that the methods were linear and selective for all matrices studied. Analysis of accuracy and precision showed adequate values, with variations lower than 10% in biological samples and lower than 2% in analytical samples. The recoveries were as high as 96% and 99% in the plasma and livers, respectively. The quantification limit of the analytical method was 1.12 ng/ml, and the limits of quantification of the bioanalytical method were 15 ng/ml and 75 ng/g for plasma and liver samples, respectively. The bioanalytical method developed was sensitive in the ranges of 15–100 ng/ml in plasma and 75–500 ng/g in liver samples and was applied to studies of biodistribution and pharmacokinetics of AlClPc.
publishDate	2011
dc.date.none.fl_str_mv	2011 2014-11-11T18:14:12Z 2014-11-11T18:14:12Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	OLIVEIRA, L. M. et al. HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. Journal of Pharmaceutical and Biomedical Analysis. V. 56, p. 70-77, 2011. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0731708511002275>. Acesso em: 20 ago. 2014. 0731-7085 http://www.repositorio.ufop.br/handle/123456789/3770 https://doi.org/10.1016/j.jpba.2011.04.016
dc.identifier.dark.fl_str_mv	ark:/61566/0013000003ckn
identifier_str_mv	OLIVEIRA, L. M. et al. HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. Journal of Pharmaceutical and Biomedical Analysis. V. 56, p. 70-77, 2011. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0731708511002275>. Acesso em: 20 ago. 2014. 0731-7085 ark:/61566/0013000003ckn
url	http://www.repositorio.ufop.br/handle/123456789/3770 https://doi.org/10.1016/j.jpba.2011.04.016
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP
instname_str	Universidade Federal de Ouro Preto (UFOP)
instacron_str	UFOP
institution	UFOP
reponame_str	Repositório Institucional da UFOP
collection	Repositório Institucional da UFOP
repository.name.fl_str_mv	Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv	repositorio@ufop.edu.br
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score	15.300719

HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.

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