The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
Toxicological effects of 25H-NBOMe and 25H-NBOH recreational drugs on zebrafish embryos and larvae at the end of 96 h exposure period were demonstrated. 25H-NBOH and 25H-NBOMe caused high embryo mortality at 80 and 100 µg mL−1, respectively. According to the decrease in the concentration tested, let...
| Autores: | , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | Brasil |
| Institución: | Universidade Federal de Minas Gerais (UFMG) |
| Repositorio: | Repositório Institucional da UFMG |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/77990 |
| Acceso en línea: | https://doi.org/10.1016/j.crtox.2021.11.002 http://hdl.handle.net/1843/77990 https://orcid.org/0000-0002-3479-0720 https://orcid.org/0000-0003-2344-5590 https://orcid.org/0000-0002-2664-4336 https://orcid.org/0000-0002-1873-5342 https://orcid.org/0000-0001-9138-8466 https://orcid.org/0000-0002-9525-5123 https://orcid.org/0000-0001-5438-1919 |
| Access Level: | acceso abierto |
| Palabra clave: | Phenethylamines Ilicit Drugs DNA interaction Zebra fish NBOHs NBOMes Drogas - Toxicologia Fenetilamina Drogas - Uso recreativo DNA Alucinógenos Espectroscopia de fluorescência Espectroscopia de ressonância nuclear |
| Sumario: | Toxicological effects of 25H-NBOMe and 25H-NBOH recreational drugs on zebrafish embryos and larvae at the end of 96 h exposure period were demonstrated. 25H-NBOH and 25H-NBOMe caused high embryo mortality at 80 and 100 µg mL−1, respectively. According to the decrease in the concentration tested, lethality decreased while non-lethal effects were predominant up to 10 and 50 µg mL−1 of 25H-NBOH and 25H-NBOMe, respectively, including spine malformation, egg hatching delay, body malformation, otolith malformation, pericardial edema, and blood clotting. We can disclose that these drugs have an affinity for DNA in vitro using biophysical spectroscopic assays and molecular modeling methods. The experiments demonstrated that 25H-NBOH and 25H-NBOMe bind to the unclassical major groove of ctDNA with a binding constant of 27.00 × 104 M−1 and 5.27 × 104 M−1, respectively. Furthermore, these interactions lead to conformational changes in the DNA structure. Therefore, the results observed in the zebrafish embryos and DNA may be correlated. |
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