Effect of potassium availability on soybean metabolism by integrated metabolomics and ionomics analysis
Potassium (K+) has vital physiological functions in plants and its availability can impact the tolerance of species to biotic and abiotic stress conditions. Limited studies have investigated the effect of K+ fertilization on soybean metabolism. Using integrated omics, ionomics and metabolomics, we i...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/236878 |
| Acceso en línea: | http://hdl.handle.net/11449/236878 |
| Access Level: | acceso abierto |
| Palabra clave: | Glycine max Fabaceae Potassium deficiency Phytoalexins Specialised metabolism Abiotic stress Metabolomics Ionomics |
| Sumario: | Potassium (K+) has vital physiological functions in plants and its availability can impact the tolerance of species to biotic and abiotic stress conditions. Limited studies have investigated the effect of K+ fertilization on soybean metabolism. Using integrated omics, ionomics and metabolomics, we investigated the response of field-grown soybean (Glycine max) to four rates of soil K+ availability: very low (KVL), low (KL), medium (KM), and very high (KVH). Soybean trifoliate leaf (V7) and pod tissue (R5.5) extracts were analysed by ultra-performance liquid chromatography coupled to high-resolution mass spectrometry (UPLC-HRMS) and inductively coupled plasma optical emission spectroscopy (ICP-OES). Multivariate analyses showed that 51 compounds of 19 metabolic pathway maps were regulated in response to K+ availability. The soybean yield parameters also were influenced in plants under very low (KVL; 2211 kg ha-1) and low (KL; 3737 kg ha-1) differing from KM and KVH (4093 and 4096 kg ha-1, respectively) treatments. Under very low potassium availability, soybean plants promoted the accumulation of Ca2+, Mg2+, Fe2+, Cu2+, and B in young and old leaves. Not only, isoflavones, coumestans, pterocarpans, and soyasaponins also were elicited in severely K+ deficient trifoliate leaves, which can be associated with oxidative and photodynamic stress status. Potassium fertilization upregulated carbohydrate, galactolipid, and flavonol glycoside biosynthesis in leaves and pod valves, while K+ deficient pod tissues showed increasing contents of amino acids, oligosaccharides, benzoic acid derivates, and isoflavones. Additionally, results demonstrate that asparagine content is higher in potassium deficient tissues, which suggests being a biomarker of K+ deficiency in soybean plants. These results demonstrate that potassium soil fertilization did not linearly contribute to changes in specialised constitutive metabolites of soybean. Altogether, this work provides a reference for improving the understanding of soybean metabolism as dependent on K+ availability. |
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