Effects of nano-sized sodium hexametaphosphate on the viability, metabolism, matrix composition, and structure of dual-species biofilms of Streptococcus mutans and Candida albicans

This study evaluated the effects of micrometric or nano-sized sodium hexametaphosphate (HMPnano), combined or not with fluoride (NaF, 1100 ppm), on dual-species biofilms of Streptococcus mutans and Candida albicans. Biofilms were treated with solutions containing the polyphosphates at 0.5% or 1.0%,...

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Detalhes bibliográficos
Autores: Sampaio, Caio [UNESP], Delbem, Alberto Carlos Botazzo [UNESP], Hosida, Thayse Yumi [UNESP], Morais, Leonardo Antônio de [UNESP], Fernandes, Ana Vitória Pereira [UNESP], Souza Neto, Francisco Nunes [UNESP], Camargo, Emerson Rodrigues de, Monteiro, Douglas Roberto, Pessan, Juliano Pelim [UNESP]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/240014
Acesso em linha:http://dx.doi.org/10.1080/08927014.2022.2064220
http://hdl.handle.net/11449/240014
Access Level:acceso abierto
Palavra-chave:biofilms
Candida albicans
fluorides
nanotechnology
Phosphates
Streptococcus mutans
Descrição
Resumo:This study evaluated the effects of micrometric or nano-sized sodium hexametaphosphate (HMPnano), combined or not with fluoride (NaF, 1100 ppm), on dual-species biofilms of Streptococcus mutans and Candida albicans. Biofilms were treated with solutions containing the polyphosphates at 0.5% or 1.0%, with/without fluoride (F), in addition to positive and negative controls. Biofilms were analysed by colony-forming units (CFU) counting, metabolic activity, production of biomass, composition of extracellular matrix, and structure. 1% HMPnano + F led to the lowest S. mutans CFU, while C. albicans CFU counts were not affected by any solution. 1% HMPnano led to the lowest metabolic activity, except for 1% HMPnano + F. All solutions promoted reductions in biofilm biomass compared to controls. Also, 1% HMPnano + F promoted the lowest concentrations of carbohydrates in the biofilm matrix, besides substantially affecting biofilms’ structure. In conclusion, HMPnano and F promoted higher antibiofilm effects compared with its micrometric counterpart for most of the parameters assessed.

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