Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds
In this observational study, phenotypic and genotypic patterns of antimicrobial resistance (AMR) in Klebsiella pneumoniae isolated from intramammary infections, clinical mastitis, fresh feces, rectal swabs, animal hindlimbs, and bulk tank milk samples from Brazilian dairy herds were investigated. In...
| Autores: | , , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | Brasil |
| Recursos: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/207530 |
| Acesso em linha: | http://dx.doi.org/10.3168/jds.2020-19569 http://hdl.handle.net/11449/207530 |
| Access Level: | acceso abierto |
| Palavra-chave: | antimicrobial resistance extended-spectrum β-lactamase Klebsiella pneumoniae mastitis |
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| dc.title.none.fl_str_mv |
Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds |
| title |
Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds |
| spellingShingle |
Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds Nobrega, Diego Borin antimicrobial resistance extended-spectrum β-lactamase Klebsiella pneumoniae mastitis |
| title_short |
Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds |
| title_full |
Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds |
| title_fullStr |
Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds |
| title_full_unstemmed |
Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds |
| title_sort |
Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds |
| dc.creator.none.fl_str_mv |
Nobrega, Diego Borin Calarga, Aline Parolin Nascimento, Leandro Costa Chande Vasconcelos, Carla Gasparotto de Lima, Eliane Martins Langoni, Helio [UNESP] Brocchi, Marcelo |
| author |
Nobrega, Diego Borin |
| author_facet |
Nobrega, Diego Borin Calarga, Aline Parolin Nascimento, Leandro Costa Chande Vasconcelos, Carla Gasparotto de Lima, Eliane Martins Langoni, Helio [UNESP] Brocchi, Marcelo |
| author_role |
author |
| author2 |
Calarga, Aline Parolin Nascimento, Leandro Costa Chande Vasconcelos, Carla Gasparotto de Lima, Eliane Martins Langoni, Helio [UNESP] Brocchi, Marcelo |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
University of Guelph Universidade Estadual de Campinas (UNICAMP) VidaVet Veterinary Analyses Laboratory Universidade Estadual Paulista (Unesp) |
| dc.subject.por.fl_str_mv |
antimicrobial resistance extended-spectrum β-lactamase Klebsiella pneumoniae mastitis |
| topic |
antimicrobial resistance extended-spectrum β-lactamase Klebsiella pneumoniae mastitis |
| description |
In this observational study, phenotypic and genotypic patterns of antimicrobial resistance (AMR) in Klebsiella pneumoniae isolated from intramammary infections, clinical mastitis, fresh feces, rectal swabs, animal hindlimbs, and bulk tank milk samples from Brazilian dairy herds were investigated. In addition, we identified specific genetic variants present among extended-spectrum β-lactamase (ESBL) producers. We obtained 169 isolates of K. pneumoniae from 2009 to 2011 on 24 Brazilian dairy farms located in 4 Brazilian states. The AMR profile of all isolates was determined using disk-diffusion assays. The antimicrobial panel included drugs commonly used as mastitis treatment in Brazilian dairy herds (gentamicin, cephalosporins, sulfamethoxazole-trimethoprim, tetracycline) as well as antimicrobials of critical importance for human health (meropenem, ceftazidime, fluoroquinolones). The K. pneumoniae isolates resistant to tetracycline, fluoroquinolones, sulfamethoxazole-trimethoprim, or chloramphenicol were screened for presence of drug-specific AMR genes [tet, qnr, aac(6')-Ib, floR, catA2, cm1A, dfr, sul] using PCR. In addition, we identified ESBL genes present among ESBL-producers by using whole genome sequencing. Genomes were assembled and annotated, and patterns of AMR genes were investigated. Resistance was commonly detected against tetracycline (22.5% of all isolates), streptomycin (20.7%), and sulfamethoxazole-trimethoprim (9.5%). Antimicrobial resistance rates were higher in K. pneumoniae isolated from intramammary infections in comparison with isolates from feces (19.2 and 0% of multidrug resistance in intramammary and fecal isolates, respectively). In contrast, no difference in AMR rates was observed when contrasting hind limbs and isolates from intramammary infections. The genes tetA, sul2, and floR were the most frequently observed AMR genes in K. pneumoniae resistant to tetracycline, sulfamethoxazole-trimethoprim, and chloramphenicol, respectively. The tetA gene was present exclusively in isolates from milk. The genes blaCTX-M8 and blaSHV-108 were present in 3 ESBL-producing K. pneumoniae, including an isolate from bulk tank milk. The 3 isolates were of sequence type 281 and had similar mobile genetic elements and virulence genes. Our study reinforced the epidemiological importance and dissemination of blaCTX-M-8 pST114 plasmid in food-producing animals in Brazil. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-06-25T10:56:45Z 2021-06-25T10:56:45Z 2021-01-01 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3168/jds.2020-19569 Journal of Dairy Science. 1525-3198 0022-0302 http://hdl.handle.net/11449/207530 10.3168/jds.2020-19569 2-s2.0-85103288211 |
| url |
http://dx.doi.org/10.3168/jds.2020-19569 http://hdl.handle.net/11449/207530 |
| identifier_str_mv |
Journal of Dairy Science. 1525-3198 0022-0302 10.3168/jds.2020-19569 2-s2.0-85103288211 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Journal of Dairy Science |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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repositoriounesp@unesp.br |
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1853672196528930816 |
| spelling |
Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herdsantimicrobial resistanceextended-spectrum β-lactamaseKlebsiella pneumoniaemastitisIn this observational study, phenotypic and genotypic patterns of antimicrobial resistance (AMR) in Klebsiella pneumoniae isolated from intramammary infections, clinical mastitis, fresh feces, rectal swabs, animal hindlimbs, and bulk tank milk samples from Brazilian dairy herds were investigated. In addition, we identified specific genetic variants present among extended-spectrum β-lactamase (ESBL) producers. We obtained 169 isolates of K. pneumoniae from 2009 to 2011 on 24 Brazilian dairy farms located in 4 Brazilian states. The AMR profile of all isolates was determined using disk-diffusion assays. The antimicrobial panel included drugs commonly used as mastitis treatment in Brazilian dairy herds (gentamicin, cephalosporins, sulfamethoxazole-trimethoprim, tetracycline) as well as antimicrobials of critical importance for human health (meropenem, ceftazidime, fluoroquinolones). The K. pneumoniae isolates resistant to tetracycline, fluoroquinolones, sulfamethoxazole-trimethoprim, or chloramphenicol were screened for presence of drug-specific AMR genes [tet, qnr, aac(6')-Ib, floR, catA2, cm1A, dfr, sul] using PCR. In addition, we identified ESBL genes present among ESBL-producers by using whole genome sequencing. Genomes were assembled and annotated, and patterns of AMR genes were investigated. Resistance was commonly detected against tetracycline (22.5% of all isolates), streptomycin (20.7%), and sulfamethoxazole-trimethoprim (9.5%). Antimicrobial resistance rates were higher in K. pneumoniae isolated from intramammary infections in comparison with isolates from feces (19.2 and 0% of multidrug resistance in intramammary and fecal isolates, respectively). In contrast, no difference in AMR rates was observed when contrasting hind limbs and isolates from intramammary infections. The genes tetA, sul2, and floR were the most frequently observed AMR genes in K. pneumoniae resistant to tetracycline, sulfamethoxazole-trimethoprim, and chloramphenicol, respectively. The tetA gene was present exclusively in isolates from milk. The genes blaCTX-M8 and blaSHV-108 were present in 3 ESBL-producing K. pneumoniae, including an isolate from bulk tank milk. The 3 isolates were of sequence type 281 and had similar mobile genetic elements and virulence genes. Our study reinforced the epidemiological importance and dissemination of blaCTX-M-8 pST114 plasmid in food-producing animals in Brazil.Department of Population Medicine Ontario Veterinary College University of GuelphDepartment of Genetics Evolution Microbiology and Immunology Institute of Biology University of Campinas (UNICAMP), CampinasCentral Laboratory for High Performance Technologies (LaCTAD) University of Campinas (UNICAMP), CampinasVidaVet Veterinary Analyses Laboratory, BotucatuDepartment of Veterinary Hygiene and Public Health São Paulo State University (UNESP), BotucatuDepartment of Veterinary Hygiene and Public Health São Paulo State University (UNESP), BotucatuUniversity of GuelphUniversidade Estadual de Campinas (UNICAMP)VidaVet Veterinary Analyses LaboratoryUniversidade Estadual Paulista (Unesp)2021-06-25T10:56:45Z2021-06-25T10:56:45Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3168/jds.2020-19569Journal of Dairy Science.1525-31980022-0302http://hdl.handle.net/11449/20753010.3168/jds.2020-195692-s2.0-85103288211Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dairy Scienceinfo:eu-repo/semantics/openAccessNobrega, Diego BorinCalarga, Aline ParolinNascimento, Leandro CostaChande Vasconcelos, Carla Gasparottode Lima, Eliane MartinsLangoni, Helio [UNESP]Brocchi, Marcelo2025-10-22T05:03:32Zoai:repositorio.unesp.br:11449/207530Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-22T05:03:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
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15.301603 |