A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction...
| Authors: | , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2013 |
| Country: | Brasil |
| Institution: | Universidade Federal de São Paulo (UNIFESP) |
| Repository: | Repositório Institucional da UNIFESP |
| Language: | English |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/7738 |
| Online Access: | http://dx.doi.org/10.1590/S0074-02762013000300020 http://repositorio.unifesp.br/handle/11600/7738 |
| Access Level: | Open access |
| Keyword: | Stenotrophomonas maltophilia identification PCR |
| Summary: | Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system. |
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