Differential Expression of Human MicroRNAs During Dengue Virus Infection in THP-1 Monocytes
Dengue virus (DENV) is the most widespread arbovirus, responsible for a wide range of clinical manifestations, varying from self-limited illness to severe hemorrhagic fever. Dengue severity is associated with host intense proinflammatory response and monocytes have been considered one of the key cel...
| Autores: | , , , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | Brasil |
| Recursos: | Universidade Federal de Minas Gerais (UFMG) |
| Repositorio: | Repositório Institucional da UFMG |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/56493 |
| Acesso em linha: | https://doi.org/10.3389/fcimb.2021.714088 http://hdl.handle.net/1843/56493 https://orcid.org/0000-0001-6235-8807 https://orcid.org/0000-0001-5180-3717 https://orcid.org/0000-0003-2622-534X https://orcid.org/0000-0002-7660-7058 https://orcid.org/0000-0002-8663-3364 https://orcid.org/0000-0002-3969-704X https://orcid.org/0000-0003-0570-750X |
| Access Level: | acceso abierto |
| Palavra-chave: | MicroRNA Monocyte Dengue Gene expression Virus-host Dengue - Epidemiologia Relação hospedeiro-virus Monocitos Genetica - Expressão |
| Resumo: | Dengue virus (DENV) is the most widespread arbovirus, responsible for a wide range of clinical manifestations, varying from self-limited illness to severe hemorrhagic fever. Dengue severity is associated with host intense proinflammatory response and monocytes have been considered one of the key cell types involved in the early steps of DENV infection and immunopathogenesis. To better understand cellular mechanisms involved in monocyte infection by DENV, we analyzed the expression levels of 754 human microRNAs in DENV-infected THP-1 cells, a human monocytic cell line. Eleven human microRNAs showed differential expression after DENV infection and gene ontology and enrichment analysis revealed biological processes potentially affected by these molecules. Five downregulated microRNAs were significantly linked to cellular response to stress, four to cell death/apoptosis, two to innate immune responses and one upregulated to vesicle mediated, TGF-β signaling, phosphatidylinositol mediated signaling, lipid metabolism process and blood coagulation. |
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