Contrasting the Chromosomal Organization of repetitive DNAs in two crickets of Gryllidae with highly divergent karyotypes

A large percentage of eukaryotic genomes consist of repetitive DNA that plays an important role in the organization, size and evolution. In the case of crickets, chromosomal variability has been found using classical cytogenetics, but almost no information concerning the organization of their repeti...

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Autores: Carvalho, Carlos Roberto, Palacios-Gimenez, Octavio M., Soares, Fernanda Aparecida Ferrari, Cabral-de-Mello, Diogo C.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Brasil
Institución:Universidade Federal de Viçosa (UFV)
Repositorio:LOCUS Repositório Institucional da UFV
Idioma:inglés
OAI Identifier:oai:locus.ufv.br:123456789/22808
Acceso en línea:https://doi.org/10.1371/journal.pone.0143540
http://www.locus.ufv.br/handle/123456789/22808
Access Level:acceso abierto
Palabra clave:Chromosomal organization of DNAs
Gryllidae crickets
Divergent karyotypes
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spelling Contrasting the Chromosomal Organization of repetitive DNAs in two crickets of Gryllidae with highly divergent karyotypesChromosomal organization of DNAsGryllidae cricketsDivergent karyotypesA large percentage of eukaryotic genomes consist of repetitive DNA that plays an important role in the organization, size and evolution. In the case of crickets, chromosomal variability has been found using classical cytogenetics, but almost no information concerning the organization of their repetitive DNAs is available. To better understand the chromosomal organization and diversification of repetitive DNAs in crickets, we studied the chromosomes of two Gryllidae species with highly divergent karyotypes, i.e., 2n(♂) = 29,X0 (Gryllus assimilis) and 2n = 9, neo-X1X2Y (Eneoptera surinamensis). The analyses were performed using classical cytogenetic techniques, repetitive DNA mapping and genome-size estimation. Conserved characteristics were observed, such as the occurrence of a small number of clusters of rDNAs and U snDNAs, in contrast to the multiple clusters/dispersal of the H3 histone genes. The positions of U2 snDNA and 18S rDNA are also conserved, being intermingled within the largest autosome. The distribution and base-pair composition of the heterochromatin and repetitive DNA pools of these organisms differed, suggesting reorganization. Although the microsatellite arrays had a similar distribution pattern, being dispersed along entire chromosomes, as has been observed in some grasshopper species, a band-like pattern was also observed in the E. surinamensis chromosomes, putatively due to their amplification and clustering. In addition to these differences, the genome of E. surinamensis is approximately 2.5 times larger than that of G. assimilis, which we hypothesize is due to the amplification of repetitive DNAs. Finally, we discuss the possible involvement of repetitive DNAs in the differentiation of the neo-sex chromosomes of E. surinamensis, as has been reported in other eukaryotic groups. This study provided an opportunity to explore the evolutionary dynamics of repetitive DNAs in two non-model species and will contribute to the understanding of chromosomal evolution in a group about which little chromosomal and genomic information is known.Plos One2018-12-17T11:42:00Z2018-12-17T11:42:00Z2015-12-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1932-6203https://doi.org/10.1371/journal.pone.0143540http://www.locus.ufv.br/handle/123456789/22808engv. 10, n. 12, p. 01– 18, dez. 2015info:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVCarvalho, Carlos RobertoPalacios-Gimenez, Octavio M.Soares, Fernanda Aparecida FerrariCabral-de-Mello, Diogo C.2024-07-12T07:02:15Zoai:locus.ufv.br:123456789/22808Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:02:15LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Contrasting the Chromosomal Organization of repetitive DNAs in two crickets of Gryllidae with highly divergent karyotypes
title Contrasting the Chromosomal Organization of repetitive DNAs in two crickets of Gryllidae with highly divergent karyotypes
spellingShingle Contrasting the Chromosomal Organization of repetitive DNAs in two crickets of Gryllidae with highly divergent karyotypes
Carvalho, Carlos Roberto
Chromosomal organization of DNAs
Gryllidae crickets
Divergent karyotypes
title_short Contrasting the Chromosomal Organization of repetitive DNAs in two crickets of Gryllidae with highly divergent karyotypes
title_full Contrasting the Chromosomal Organization of repetitive DNAs in two crickets of Gryllidae with highly divergent karyotypes
title_fullStr Contrasting the Chromosomal Organization of repetitive DNAs in two crickets of Gryllidae with highly divergent karyotypes
title_full_unstemmed Contrasting the Chromosomal Organization of repetitive DNAs in two crickets of Gryllidae with highly divergent karyotypes
title_sort Contrasting the Chromosomal Organization of repetitive DNAs in two crickets of Gryllidae with highly divergent karyotypes
dc.creator.none.fl_str_mv Carvalho, Carlos Roberto
Palacios-Gimenez, Octavio M.
Soares, Fernanda Aparecida Ferrari
Cabral-de-Mello, Diogo C.
author Carvalho, Carlos Roberto
author_facet Carvalho, Carlos Roberto
Palacios-Gimenez, Octavio M.
Soares, Fernanda Aparecida Ferrari
Cabral-de-Mello, Diogo C.
author_role author
author2 Palacios-Gimenez, Octavio M.
Soares, Fernanda Aparecida Ferrari
Cabral-de-Mello, Diogo C.
author2_role author
author
author
dc.subject.por.fl_str_mv Chromosomal organization of DNAs
Gryllidae crickets
Divergent karyotypes
topic Chromosomal organization of DNAs
Gryllidae crickets
Divergent karyotypes
description A large percentage of eukaryotic genomes consist of repetitive DNA that plays an important role in the organization, size and evolution. In the case of crickets, chromosomal variability has been found using classical cytogenetics, but almost no information concerning the organization of their repetitive DNAs is available. To better understand the chromosomal organization and diversification of repetitive DNAs in crickets, we studied the chromosomes of two Gryllidae species with highly divergent karyotypes, i.e., 2n(♂) = 29,X0 (Gryllus assimilis) and 2n = 9, neo-X1X2Y (Eneoptera surinamensis). The analyses were performed using classical cytogenetic techniques, repetitive DNA mapping and genome-size estimation. Conserved characteristics were observed, such as the occurrence of a small number of clusters of rDNAs and U snDNAs, in contrast to the multiple clusters/dispersal of the H3 histone genes. The positions of U2 snDNA and 18S rDNA are also conserved, being intermingled within the largest autosome. The distribution and base-pair composition of the heterochromatin and repetitive DNA pools of these organisms differed, suggesting reorganization. Although the microsatellite arrays had a similar distribution pattern, being dispersed along entire chromosomes, as has been observed in some grasshopper species, a band-like pattern was also observed in the E. surinamensis chromosomes, putatively due to their amplification and clustering. In addition to these differences, the genome of E. surinamensis is approximately 2.5 times larger than that of G. assimilis, which we hypothesize is due to the amplification of repetitive DNAs. Finally, we discuss the possible involvement of repetitive DNAs in the differentiation of the neo-sex chromosomes of E. surinamensis, as has been reported in other eukaryotic groups. This study provided an opportunity to explore the evolutionary dynamics of repetitive DNAs in two non-model species and will contribute to the understanding of chromosomal evolution in a group about which little chromosomal and genomic information is known.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-02
2018-12-17T11:42:00Z
2018-12-17T11:42:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1932-6203
https://doi.org/10.1371/journal.pone.0143540
http://www.locus.ufv.br/handle/123456789/22808
identifier_str_mv 1932-6203
url https://doi.org/10.1371/journal.pone.0143540
http://www.locus.ufv.br/handle/123456789/22808
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 10, n. 12, p. 01– 18, dez. 2015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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