Progesterone and vitamin D downregulate the activation of the NLRP1/NLRP3 inflammasomes and TLR4-MyD88-NF-κB pathway in monocytes from pregnant women with preeclampsia

This study evaluated the in vitro modulatory effect of progesterone (PG) and vitamin D (VD) on NLRP1/NLRP3 inflammasomes and TLR4/NF-κB pathway in monocytes from pregnant women with preeclampsia (PE). Monocytes from 20 preeclamptic and 20 normotensive (NT) pregnant women, and THP-1 cells were cultur...

Descripción completa

Detalles Bibliográficos
Autores: Matias, Mariana Leticia [UNESP], Romao-Veiga, Mariana [UNESP], Ribeiro, Vanessa Rocha [UNESP], Nunes, Priscila Rezeck [UNESP], Gomes, Virginia Juliani [UNESP], Devides, Amanda Carreira [UNESP], Borges, Vera Therezinha [UNESP], Romagnoli, Graziela Goretti, Peracoli, Jose Carlos [UNESP], Peracoli, Maria Terezinha [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/207259
Acceso en línea:http://dx.doi.org/10.1016/j.jri.2021.103286
http://hdl.handle.net/11449/207259
Access Level:acceso abierto
Palabra clave:Monocytes
NLRP1/NLRP3 inflammasomes
Preeclampsia
Progesterone
Vitamin D
Descripción
Sumario:This study evaluated the in vitro modulatory effect of progesterone (PG) and vitamin D (VD) on NLRP1/NLRP3 inflammasomes and TLR4/NF-κB pathway in monocytes from pregnant women with preeclampsia (PE). Monocytes from 20 preeclamptic and 20 normotensive (NT) pregnant women, and THP-1 cells were cultured with/without hyaluronan (HA), PG, or VD to determine gene and protein expression of TLR4 receptor, phosphorylated NF-κB, IκBα, TLR4, MYD88, NF-κB, NLRP1, NLRP3, caspase-1, IL-1β, IL-18, TNF-α, and IL-10. Higher endogenous activation of inflammatory genes and higher protein expression of TLR4 and NF-κB was detected in monocytes of PE group and decreased after PG or VD treatment. Monocyte from PE stimulated with HA increased while treatment with PG or VD decreased the expression of genes and proteins related to the inflammasomes. THP-1 cells showed a similar immune response profile as monocytes from PE. These results demonstrate that PG and VD play an immunomodulatory role in monocyte activation.

Similares en LA Referencia