OBTENTION METHODS OF RESPIRATORY TRACT CELSS IN HEALTHY SHEEP BY THE TRACHEOBRONCHEAL LAVAGE TECHNIQUE BY NASOTRACHEAL VIA
The technique of pulmonary cells obtention by nasotracheal intubation was evaluated and the cellular population of 19 healthy sheep was characterized. The animals were hold standing still with head and neck strait; aligned with the vertebral spine and a silicone tube guide till the carina, to introd...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2008 |
| País: | Brasil |
| Institución: | Universidade Federal de Goiás (UFG) |
| Repositorio: | Ciência animal brasileira (Online) |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.revistas.ufg.br:article/1049 |
| Acceso en línea: | https://revistas.ufg.br/vet/article/view/1049 |
| Access Level: | acceso abierto |
| Palabra clave: | Sanidade Animal ovinos lavado traqueobrônquico citologia |
| Sumario: | The technique of pulmonary cells obtention by nasotracheal intubation was evaluated and the cellular population of 19 healthy sheep was characterized. The animals were hold standing still with head and neck strait; aligned with the vertebral spine and a silicone tube guide till the carina, to introduce a lower caliber tube for precede the tracheobronchial lavage. The average counting of nucleated cells was 64.650 ± 49.674. The cytological analyses of the samples slowed by the average of obtained percentages: 62.74% of macrophages, 15.01% cylindrical epithelial cells, 20.04% of neutrophills, 1.44 of lymphocytes and 0.77% of eosinophills. It was concluded that this methods of sample obtention by nasotracheal intubation to reach the tracheobronchial region was efficient to cytological characterization and cellular differentiation in the obtained samples, being well supported by the animals. KEY WORDS: Citocentrifugation, cytology, tracheobronchial lavage, sheep. |
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