Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge

Avian infectious bronchitis virus (IBV) is one of the most important viral diseases of poultry. The mucosa of upper respiratory tract, specially the trachea, is the primary replication site for this virus. However, conventional inactivate IBV vaccines usually elicit reduced mucosal immune responses...

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Autores: Lopes, Priscila Diniz [UNESP], Okino, Cintia Hiromi, Fernando, Filipe Santos [UNESP], Pavani, Caren [UNESP], Casagrande, Viviane Mariguela [UNESP], Lopez, Renata F.V., Montassier, Maria de Fátima Silva [UNESP], Montassier, Helio José [UNESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/179751
Acceso en línea:http://dx.doi.org/10.1016/j.vaccine.2018.03.065
http://hdl.handle.net/11449/179751
Access Level:acceso abierto
Palabra clave:Avian infectious bronchitis virus
BR-I genotype
Chitosan nanoparticles
Inactivated vaccine
Mucosal immune responses
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spelling Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challengeAvian infectious bronchitis virusBR-I genotypeChitosan nanoparticlesInactivated vaccineMucosal immune responsesAvian infectious bronchitis virus (IBV) is one of the most important viral diseases of poultry. The mucosa of upper respiratory tract, specially the trachea, is the primary replication site for this virus. However, conventional inactivate IBV vaccines usually elicit reduced mucosal immune responses and local protection. Thus, an inactivated IBV vaccine containing BR-I genotype strain encapsulated in chitosan nanoparticles (IBV-CS) was produced by ionic gelation method to be administered by oculo-nasal route to chickens. IBV-CS vaccine administered alone resulted in markedly mucosal immune responses, characterized by high levels of anti-IBV IgA isotype antibodies and IFNγ gene expression at 1dpi. The association of live attenuated Massachusetts IBV and IBV-CS vaccine also induced strong mucosal immune responses, though a switch from IgA isotype to IgG was observed, and IFNγ gene expression peak was late (at 5 dpi). Efficacy of IBV-CS was evaluated by tracheal ciliostasis analysis, histopathology examination, and viral load determination in the trachea and kidney. The results indicated that IBV-CS vaccine administered alone or associated with a live attenuated heterologous vaccine induced both humoral and cell-mediated immune responses at the primary site of viral replication, and provided an effective protection against IBV infection at local (trachea) and systemic (kidney) sites.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castelane, S/N – Vila Industrial, JaboticabalEmbrapa Pecuária Sudeste Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Rodovia Washington Luiz, Km 234 s/n – Fazenda Canchim, São CarlosFaculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo (USP), Avenida Bandeirantes, 3.900 – Monte Alegre, Ribeirão PretoFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castelane, S/N – Vila Industrial, JaboticabalCNPq: 140100/2015-6Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade de São Paulo (USP)2018-12-11T17:36:36Z2018-12-11T17:36:36Z2018-05-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2630-2636application/pdfhttp://dx.doi.org/10.1016/j.vaccine.2018.03.065Vaccine, v. 36, n. 19, p. 2630-2636, 2018.1873-25180264-410Xhttp://hdl.handle.net/11449/17975110.1016/j.vaccine.2018.03.0652-s2.0-850451240962-s2.0-85045124096.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVaccineinfo:eu-repo/semantics/openAccessLopes, Priscila Diniz [UNESP]Okino, Cintia HiromiFernando, Filipe Santos [UNESP]Pavani, Caren [UNESP]Casagrande, Viviane Mariguela [UNESP]Lopez, Renata F.V.Montassier, Maria de Fátima Silva [UNESP]Montassier, Helio José [UNESP]2024-06-07T13:01:27Zoai:repositorio.unesp.br:11449/179751Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-06-07T13:01:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge
title Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge
spellingShingle Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge
Lopes, Priscila Diniz [UNESP]
Avian infectious bronchitis virus
BR-I genotype
Chitosan nanoparticles
Inactivated vaccine
Mucosal immune responses
title_short Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge
title_full Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge
title_fullStr Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge
title_full_unstemmed Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge
title_sort Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge
dc.creator.none.fl_str_mv Lopes, Priscila Diniz [UNESP]
Okino, Cintia Hiromi
Fernando, Filipe Santos [UNESP]
Pavani, Caren [UNESP]
Casagrande, Viviane Mariguela [UNESP]
Lopez, Renata F.V.
Montassier, Maria de Fátima Silva [UNESP]
Montassier, Helio José [UNESP]
author Lopes, Priscila Diniz [UNESP]
author_facet Lopes, Priscila Diniz [UNESP]
Okino, Cintia Hiromi
Fernando, Filipe Santos [UNESP]
Pavani, Caren [UNESP]
Casagrande, Viviane Mariguela [UNESP]
Lopez, Renata F.V.
Montassier, Maria de Fátima Silva [UNESP]
Montassier, Helio José [UNESP]
author_role author
author2 Okino, Cintia Hiromi
Fernando, Filipe Santos [UNESP]
Pavani, Caren [UNESP]
Casagrande, Viviane Mariguela [UNESP]
Lopez, Renata F.V.
Montassier, Maria de Fátima Silva [UNESP]
Montassier, Helio José [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade de São Paulo (USP)
dc.subject.por.fl_str_mv Avian infectious bronchitis virus
BR-I genotype
Chitosan nanoparticles
Inactivated vaccine
Mucosal immune responses
topic Avian infectious bronchitis virus
BR-I genotype
Chitosan nanoparticles
Inactivated vaccine
Mucosal immune responses
description Avian infectious bronchitis virus (IBV) is one of the most important viral diseases of poultry. The mucosa of upper respiratory tract, specially the trachea, is the primary replication site for this virus. However, conventional inactivate IBV vaccines usually elicit reduced mucosal immune responses and local protection. Thus, an inactivated IBV vaccine containing BR-I genotype strain encapsulated in chitosan nanoparticles (IBV-CS) was produced by ionic gelation method to be administered by oculo-nasal route to chickens. IBV-CS vaccine administered alone resulted in markedly mucosal immune responses, characterized by high levels of anti-IBV IgA isotype antibodies and IFNγ gene expression at 1dpi. The association of live attenuated Massachusetts IBV and IBV-CS vaccine also induced strong mucosal immune responses, though a switch from IgA isotype to IgG was observed, and IFNγ gene expression peak was late (at 5 dpi). Efficacy of IBV-CS was evaluated by tracheal ciliostasis analysis, histopathology examination, and viral load determination in the trachea and kidney. The results indicated that IBV-CS vaccine administered alone or associated with a live attenuated heterologous vaccine induced both humoral and cell-mediated immune responses at the primary site of viral replication, and provided an effective protection against IBV infection at local (trachea) and systemic (kidney) sites.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:36:36Z
2018-12-11T17:36:36Z
2018-05-03
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vaccine.2018.03.065
Vaccine, v. 36, n. 19, p. 2630-2636, 2018.
1873-2518
0264-410X
http://hdl.handle.net/11449/179751
10.1016/j.vaccine.2018.03.065
2-s2.0-85045124096
2-s2.0-85045124096.pdf
url http://dx.doi.org/10.1016/j.vaccine.2018.03.065
http://hdl.handle.net/11449/179751
identifier_str_mv Vaccine, v. 36, n. 19, p. 2630-2636, 2018.
1873-2518
0264-410X
10.1016/j.vaccine.2018.03.065
2-s2.0-85045124096
2-s2.0-85045124096.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Vaccine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2630-2636
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,301603

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