Sustainable approach to recover β-carotene and astaxanthin from Phaffia rhodozyma grown in a stirred-tank bioreactor under the influence of magnetic fields

This study aimed to produce carotenoids by Phaffia rhodozyma in a stirred-tank bioreactor under the influence of magnetic fields (MF) and to evaluate a sustainable approach to recover them from the yeast biomass. MF application proved to be effective in increasing 8.6 and 22.9 % of β-carotene and as...

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Bibliographic Details
Authors: Silva, Pedro Garcia Pereira, Mussagy, Cassamo U., Lima, Caio de Azevedo [UNESP], Santos-Ebinuma, Valéria C. [UNESP], Burkert, Janaína Fernandes de Medeiros, Santos, Lucielen Oliveira
Format: article
Status:Published version
Publication Date:2023
Country:Brasil
Institution:Universidade Estadual Paulista (UNESP)
Repository:Repositório Institucional da UNESP
Language:English
OAI Identifier:oai:repositorio.unesp.br:11449/300266
Online Access:http://dx.doi.org/10.1016/j.biortech.2023.129906
https://hdl.handle.net/11449/300266
Access Level:Open access
Keyword:Carotenogenic extract
Carotenoid production
Magnetic effect
Phaffia rhodozyma
Protic ionic liquid
Description
Summary:This study aimed to produce carotenoids by Phaffia rhodozyma in a stirred-tank bioreactor under the influence of magnetic fields (MF) and to evaluate a sustainable approach to recover them from the yeast biomass. MF application proved to be effective in increasing 8.6 and 22.9 % of β-carotene and astaxanthin production, respectively. Regarding solid–liquid extraction (SLE), the ability of aqueous and ethanolic solutions of protic ionic liquids (PILs) was determined. β-carotene and astaxanthin recovery yields increased with the anion alkyl chain length hydrophobicity. [Pro][Oct]:EtOH (50 % v v-1) was selected as the effective solvent. Moreover, it led to improvement in carotenoid stability at different storage temperatures over time in comparison with the control. This study is one of the first to describe an effective and sustainable approach to move carotenoid production from shake flasks to a bioreactor under the influence of MF and recover carotenoids from P. rhodozyma biomass.

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