Distribution of mating-type alleles and M13 PCR markers in the black leaf spot fungus Mycosphaerella fijiensis of bananas in Brazil
The fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system an...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Brasil |
| Institución: | Instituto Nacional de Pesquisas da Amazônia (INPA) |
| Repositorio: | Repositório Institucional do INPA |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio:1/14995 |
| Acceso en línea: | https://repositorio.inpa.gov.br/handle/1/14995 |
| Access Level: | acceso abierto |
| Palabra clave: | Bacteriophage Dna Fungal Protein Microsatellite Dna Allele Banana Brasil Controlled Study Dna Fingerprinting Fungal Gene Fungal Plant Disease Fungus Isolation Gene Sequence Genetic Distance Genetic Marker Polymorphism, Genetic Genetic Similarity Genetic Variability Geographic Origin Inter Simple Sequence Repeat Mat1 1 Gene Mat1 2 Gene Mating Type Mycosphaerella Mycosphaerella Fijiensis Nonhuman Plant Leaf Polymerase Chain Reaction Retroposon Species Distribution Ascomycetes Bacteriophage M13 Genetics Methodology Microbiology Musa Plant Disease Variable Number Of Tandem Repeat Alleles Ascomycota Fungal Proteins Genes, Mating Type, Fungal Genetic Markers Microsatellite Repeats Minisatellite Repeats Plant Diseases Plant Leaves |
| Sumario: | The fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system and its genetic variability using M13 phage DNA markers. We found a 1:1 distribution of mating-type alleles, indicating MAT1-1 and MAT1-2 idiomorphs. A polymorphism analysis using three different primers for M13 markers showed that only the M13 minisatellite primers generated polymorphic products. We then utilized this polymorphism to characterize 40 isolates from various Brazilian states. The largest genetic distances were found between isolates from the same location and between isolates from different parts of the country. Therefore, there was no correlation between the genetic similarity and the geographic origin of the isolates. The M13 marker was used to generate genetic fingerprints for five isolates; these fingerprints were compared with the band profiles obtained from inter-simple sequence repeat (UBC861) and inter-retrotransposon amplified polymorphism analyses. We found that the M13 marker was more effective than the other two markers for differentiating these isolates. © FUNPEC-RP. |
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