Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops
Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access....
| Autores: | , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | Brasil |
| Recursos: | Instituto Evandro Chagas (IEC) |
| Repositorio: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
| Idioma: | inglés |
| OAI Identifier: | oai:patua.iec.gov.br:iec/2431 |
| Acesso em linha: | http://patua.iec.gov.br/handle/iec/2431 |
| Access Level: | acceso abierto |
| Palavra-chave: | Células da Medula Óssea / citologia Células da Medula Óssea / fisiologia Separação Celular / veterinária Modelos Animais de Doenças Separação Celular / métodos Centrifugação com Gradiente de Concentração / métodos |
| Resumo: | Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy. |
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