Detection of carbapenemase activity directly from blood culture vials using MALDI-TOF MS: a quick answer for the right decision

Recently, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was successfully applied for the detection of carbapenemase activity directly from Gram-negative colonies. Based on this principle, we evaluated the performance of MALDI-TOF MS for rapid detection o...

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Detalles Bibliográficos
Autores: Carvalhaes, Cecilia G. [UNIFESP], Cayô, Rodrigo [UNIFESP], Visconde, Marina F. [UNIFESP], Barone, Talita [UNIFESP], Frigatto, Eliete A. M. [UNIFESP], Okamoto, Debora [UNIFESP], Assis, Diego M. [UNIFESP], Juliano, Luiz [UNIFESP], Machado, Antonia M. O. [UNIFESP], Gales, Ana C. [UNIFESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/38010
Acceso en línea:http://dx.doi.org/10.1093/jac/dku094
http://repositorio.unifesp.br/handle/11600/38010
Access Level:acceso abierto
Palabra clave:KPC
OXA-type
oxacillinases
metallo-beta-lactamases
serine-beta-lactamases
Descripción
Sumario:Recently, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was successfully applied for the detection of carbapenemase activity directly from Gram-negative colonies. Based on this principle, we evaluated the performance of MALDI-TOF MS for rapid detection of carbapenemase activity directly from positive blood culture vials.A total of 100 blood culture vials were randomly selected. MALDI-TOF MS carbapenemase assay results were confirmed by the detection of carbapenemase-encoding genes.A total of 110 bacterial isolates were recovered. the MALDI-TOF MS carbapenemase assay identified 21 of 29 (72.4%) of the carbapenemase-producing isolates directly from the blood culture vials, especially those encoding KPC-2 (100%) and SPM-1 (100%), after a 4 h incubation period. Although the majority of OXA-23-producing Acinetobacter baumannii isolates were not identified on day 1, all isolates were identified as carbapenemase producers directly from the colony on the next day.The MALDI-TOF MS carbapenemase assay is a feasible and rapid test to identify carbapenemase activity directly from blood culture vials. It may contribute to faster readjustment of empirical antimicrobial therapy and implementation of infection control measures.

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