Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device
In this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/179299 |
| Acceso en línea: | http://dx.doi.org/10.1016/j.aca.2017.10.018 http://hdl.handle.net/11449/179299 |
| Access Level: | acceso abierto |
| Palabra clave: | Creatinine Digital image Paper-based microfluidic analytical device (μPAD) Renal function Uric acid Urine |
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Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical deviceCreatinineDigital imagePaper-based microfluidic analytical device (μPAD)Renal functionUric acidUrineIn this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (CRN) and uric acid (UA) in urine samples. The microfluidic paper-based analytical device (μPAD) consists of a main channel with three identical arms, each containing a circular testing zone and a circular uptake zone. Creatinine detection is based on the Jaffé reaction, in which CRN reacts with picrate to form an orange-red product. Uric acid quantification is based on the reduction of Fe3+ to Fe2+ by UA, which is detected in a colorimetric reaction using 1,10-phenanthroline. Under optimum conditions, obtained through chemometrics, the concentrations of the analytes showed good linear correlations with the effective intensities, and the method presented satisfactory repeatability. The limits of detection and the linear ranges, respectively, were 15.7 mg L−1 and 50–600 mg L−1 for CRN and 16.5 mg L−1 and 50–500 mg L−1 for UA. There were no statistically significant differences between the results obtained using the μPAD and a chromatographic comparative method (Student's t-test at 95% confidence level).Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Instituto de Química Universidade Estadual Paulista “Julio de Mesquita Filho”- UNESP, R. Prof. Francisco Degni 55, P.O. Box 355Instituto de Química de São Carlos Universidade de São Paulo, Av. Trabalhador São-carlense, 400Instituto de Química Universidade Estadual Paulista “Julio de Mesquita Filho”- UNESP, R. Prof. Francisco Degni 55, P.O. Box 355CNPq: 132646/2014-5CAPES: 2014/17749-7FAPESP: 2015/21733-1CNPq: 308788/2015-0Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)2018-12-11T17:34:37Z2018-12-11T17:34:37Z2018-01-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16-23application/pdfhttp://dx.doi.org/10.1016/j.aca.2017.10.018Analytica Chimica Acta, v. 997, p. 16-23.1873-43240003-2670http://hdl.handle.net/11449/17929910.1016/j.aca.2017.10.0182-s2.0-850323331872-s2.0-85032333187.pdf5978908591853524Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnalytica Chimica Acta1,512info:eu-repo/semantics/openAccessRossini, Eduardo Luiz [UNESP]Milani, Maria Izabel [UNESP]Carrilho, EmanuelPezza, Leonardo [UNESP]Pezza, Helena Redigolo [UNESP]2025-05-28T06:57:49Zoai:repositorio.unesp.br:11449/179299Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-05-28T06:57:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device |
| title |
Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device |
| spellingShingle |
Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device Rossini, Eduardo Luiz [UNESP] Creatinine Digital image Paper-based microfluidic analytical device (μPAD) Renal function Uric acid Urine |
| title_short |
Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device |
| title_full |
Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device |
| title_fullStr |
Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device |
| title_full_unstemmed |
Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device |
| title_sort |
Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device |
| dc.creator.none.fl_str_mv |
Rossini, Eduardo Luiz [UNESP] Milani, Maria Izabel [UNESP] Carrilho, Emanuel Pezza, Leonardo [UNESP] Pezza, Helena Redigolo [UNESP] |
| author |
Rossini, Eduardo Luiz [UNESP] |
| author_facet |
Rossini, Eduardo Luiz [UNESP] Milani, Maria Izabel [UNESP] Carrilho, Emanuel Pezza, Leonardo [UNESP] Pezza, Helena Redigolo [UNESP] |
| author_role |
author |
| author2 |
Milani, Maria Izabel [UNESP] Carrilho, Emanuel Pezza, Leonardo [UNESP] Pezza, Helena Redigolo [UNESP] |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
| dc.subject.por.fl_str_mv |
Creatinine Digital image Paper-based microfluidic analytical device (μPAD) Renal function Uric acid Urine |
| topic |
Creatinine Digital image Paper-based microfluidic analytical device (μPAD) Renal function Uric acid Urine |
| description |
In this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (CRN) and uric acid (UA) in urine samples. The microfluidic paper-based analytical device (μPAD) consists of a main channel with three identical arms, each containing a circular testing zone and a circular uptake zone. Creatinine detection is based on the Jaffé reaction, in which CRN reacts with picrate to form an orange-red product. Uric acid quantification is based on the reduction of Fe3+ to Fe2+ by UA, which is detected in a colorimetric reaction using 1,10-phenanthroline. Under optimum conditions, obtained through chemometrics, the concentrations of the analytes showed good linear correlations with the effective intensities, and the method presented satisfactory repeatability. The limits of detection and the linear ranges, respectively, were 15.7 mg L−1 and 50–600 mg L−1 for CRN and 16.5 mg L−1 and 50–500 mg L−1 for UA. There were no statistically significant differences between the results obtained using the μPAD and a chromatographic comparative method (Student's t-test at 95% confidence level). |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-12-11T17:34:37Z 2018-12-11T17:34:37Z 2018-01-02 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aca.2017.10.018 Analytica Chimica Acta, v. 997, p. 16-23. 1873-4324 0003-2670 http://hdl.handle.net/11449/179299 10.1016/j.aca.2017.10.018 2-s2.0-85032333187 2-s2.0-85032333187.pdf 5978908591853524 |
| url |
http://dx.doi.org/10.1016/j.aca.2017.10.018 http://hdl.handle.net/11449/179299 |
| identifier_str_mv |
Analytica Chimica Acta, v. 997, p. 16-23. 1873-4324 0003-2670 10.1016/j.aca.2017.10.018 2-s2.0-85032333187 2-s2.0-85032333187.pdf 5978908591853524 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Analytica Chimica Acta 1,512 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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16-23 application/pdf |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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repositoriounesp@unesp.br |
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