Influence of salt on the structure of DMPG studied by SAXS and optical microscopy

Aqueous dispersions of 50 mM dimyristoylphosphatidylglycerol (DMPG) in the presence of increasing salt concentrations (2-500 mM NaCl) were studied by small angle X-ray scattering (SAXS) and optical microscopy between 15 and 35 degrees C. SAXS data show the presence of a broad peak around q similar t...

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Detalles Bibliográficos
Autores: Fernandez, Roberto Morato, Riske, Karin do Amaral [UNIFESP], Amaral, Lia Queiroz do, Itri, Rosangela, Lamy, Maria Teresa Moura
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/30535
Acceso en línea:http://dx.doi.org/10.1016/j.bbamem.2007.12.005
http://repositorio.unifesp.br/handle/11600/30535
Access Level:acceso abierto
Palabra clave:gel-fluid phase transition
intermediate phase
charged phospholipid
DLVO theory
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spelling Influence of salt on the structure of DMPG studied by SAXS and optical microscopygel-fluid phase transitionintermediate phasecharged phospholipidDLVO theoryAqueous dispersions of 50 mM dimyristoylphosphatidylglycerol (DMPG) in the presence of increasing salt concentrations (2-500 mM NaCl) were studied by small angle X-ray scattering (SAXS) and optical microscopy between 15 and 35 degrees C. SAXS data show the presence of a broad peak around q similar to 0.12 angstrom(-1) at all temperatures and conditions, arising from the electron density contrasts within the bilayer. Up to 100 mM NaCl, this broad peak is the main feature observed in the gel and fluid phases. At higher ionic strength (250-500 mM NaCl), an incipient lamellar repeat distance around d= 90-100 angstrom is detected superimposed to the bilayer form factor. the data with high salt were fit and showed that the emergent Bragg peak is due to loose multilamellar structures, with the local order vanishing after similar to 4d. Optical microscopy revealed that up to 20 mM NaCl, DMPG is arranged in submicroscopic vesicles. Giant (loose) multilamellar vesicles (MLVs) start to appear with 50 mM NaCl, although most lipids are arranged in small vesicles. As the ionic strength increases, more and denser MLVs are seen, up to 500 mM NaCl, when MLVs are the prevailing structure. the DLVO theory could account for the experimentally found interbilayer distances. (c) 2007 Elsevier B.V. All rights reserved.Universidade Federal de São Paulo, Inst Fis, BR-05315970 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista de Medicina (EPM)Web of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)2016-01-24T13:49:40Z2016-01-24T13:49:40Z2008-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion907-916application/pdfhttp://dx.doi.org/10.1016/j.bbamem.2007.12.005Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1778, n. 4, p. 907-916, 2008.10.1016/j.bbamem.2007.12.005WOS000255228000011.pdf0005-2736http://repositorio.unifesp.br/handle/11600/30535WOS:000255228000011ark:/48912/001300001pc5jengBiochimica Et Biophysica Acta-biomembranesinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPFernandez, Roberto MoratoRiske, Karin do Amaral [UNIFESP]Amaral, Lia Queiroz doItri, RosangelaLamy, Maria Teresa Moura2024-08-08T04:21:04Zoai:repositorio.unifesp.br:11600/30535Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T04:21:04Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Influence of salt on the structure of DMPG studied by SAXS and optical microscopy
title Influence of salt on the structure of DMPG studied by SAXS and optical microscopy
spellingShingle Influence of salt on the structure of DMPG studied by SAXS and optical microscopy
Fernandez, Roberto Morato
gel-fluid phase transition
intermediate phase
charged phospholipid
DLVO theory
title_short Influence of salt on the structure of DMPG studied by SAXS and optical microscopy
title_full Influence of salt on the structure of DMPG studied by SAXS and optical microscopy
title_fullStr Influence of salt on the structure of DMPG studied by SAXS and optical microscopy
title_full_unstemmed Influence of salt on the structure of DMPG studied by SAXS and optical microscopy
title_sort Influence of salt on the structure of DMPG studied by SAXS and optical microscopy
dc.creator.none.fl_str_mv Fernandez, Roberto Morato
Riske, Karin do Amaral [UNIFESP]
Amaral, Lia Queiroz do
Itri, Rosangela
Lamy, Maria Teresa Moura
author Fernandez, Roberto Morato
author_facet Fernandez, Roberto Morato
Riske, Karin do Amaral [UNIFESP]
Amaral, Lia Queiroz do
Itri, Rosangela
Lamy, Maria Teresa Moura
author_role author
author2 Riske, Karin do Amaral [UNIFESP]
Amaral, Lia Queiroz do
Itri, Rosangela
Lamy, Maria Teresa Moura
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.subject.por.fl_str_mv gel-fluid phase transition
intermediate phase
charged phospholipid
DLVO theory
topic gel-fluid phase transition
intermediate phase
charged phospholipid
DLVO theory
description Aqueous dispersions of 50 mM dimyristoylphosphatidylglycerol (DMPG) in the presence of increasing salt concentrations (2-500 mM NaCl) were studied by small angle X-ray scattering (SAXS) and optical microscopy between 15 and 35 degrees C. SAXS data show the presence of a broad peak around q similar to 0.12 angstrom(-1) at all temperatures and conditions, arising from the electron density contrasts within the bilayer. Up to 100 mM NaCl, this broad peak is the main feature observed in the gel and fluid phases. At higher ionic strength (250-500 mM NaCl), an incipient lamellar repeat distance around d= 90-100 angstrom is detected superimposed to the bilayer form factor. the data with high salt were fit and showed that the emergent Bragg peak is due to loose multilamellar structures, with the local order vanishing after similar to 4d. Optical microscopy revealed that up to 20 mM NaCl, DMPG is arranged in submicroscopic vesicles. Giant (loose) multilamellar vesicles (MLVs) start to appear with 50 mM NaCl, although most lipids are arranged in small vesicles. As the ionic strength increases, more and denser MLVs are seen, up to 500 mM NaCl, when MLVs are the prevailing structure. the DLVO theory could account for the experimentally found interbilayer distances. (c) 2007 Elsevier B.V. All rights reserved.
publishDate 2008
dc.date.none.fl_str_mv 2008-04-01
2016-01-24T13:49:40Z
2016-01-24T13:49:40Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.bbamem.2007.12.005
Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1778, n. 4, p. 907-916, 2008.
10.1016/j.bbamem.2007.12.005
WOS000255228000011.pdf
0005-2736
http://repositorio.unifesp.br/handle/11600/30535
WOS:000255228000011
dc.identifier.dark.fl_str_mv ark:/48912/001300001pc5j
url http://dx.doi.org/10.1016/j.bbamem.2007.12.005
http://repositorio.unifesp.br/handle/11600/30535
identifier_str_mv Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1778, n. 4, p. 907-916, 2008.
10.1016/j.bbamem.2007.12.005
WOS000255228000011.pdf
0005-2736
WOS:000255228000011
ark:/48912/001300001pc5j
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochimica Et Biophysica Acta-biomembranes
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 907-916
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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score 15,301629

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