Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum)
Tambaqui (Colossoma macropomum) are a model species for tropical fish physiology, but details are lacking about their ionoregulatory response to acid waters. To provide specifics, we measured unidirectional Na+ fluxes in low pH waters. Sodium influx (JinNa) was uninhibited during acute exposure to p...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/205887 |
| Acceso en línea: | http://dx.doi.org/10.1007/s00360-021-01349-x http://hdl.handle.net/11449/205887 |
| Access Level: | acceso abierto |
| Palabra clave: | Fish Ion regulation Low pH |
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Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum)FishIon regulationLow pHTambaqui (Colossoma macropomum) are a model species for tropical fish physiology, but details are lacking about their ionoregulatory response to acid waters. To provide specifics, we measured unidirectional Na+ fluxes in low pH waters. Sodium influx (JinNa) was uninhibited during acute exposure to pH 4.5 and 3.5, and Na efflux (JoutNa) rose only slightly at pH 3.5; net Na+ flux (JnetNa) remained positive at all pH. Similarly, during 24 h transfer to pH 3.5 JinNa, JoutNa, and JnetNa were unchanged at all times. Taking a closer look at the mechanism of Na+ transport in the gills of tambaqui we found that JinNa was uninhibited by HMA, a Na+/H+-exchanger blocker, and Benzamil, a Na+-channel inhibitor, casting doubt on their role in Na+ uptake in this fish. Measurement of Na+/K+-ATPase (NKA) and H+-ATPase (VHA) activity showed that neither changed at low pH compared to measurements at pH 6.5. Western blot analysis of ATPase expression saw no changes in amount of NKA and VHA at low pH, and immunohistochemistry showed expression of both NKA and VHA on lamellae and interlamellar region of tambaqui gills and that both proteins co-localized to the same gill cells. Location of expression also did not change in low pH water. Amazingly, tambaqui seem unaffected by pH 3.5 water, making them one of the most acid-tolerant fish species examined so far. In addition, they appear to share key ionoregulatory traits with other fish of the order Characiformes, which suggest a common origin for the ionoregulatory attributes.Department of Biology University of San Diego, 5998 Alcalá ParkBiosciences Institute São Paulo State University-UNESP, Coastal CampusLaboratório de Ecofisiologia e Evolução Molecular Instituto Nacional de Pesquisas da Amazônia (INPA)Division of Biological Sciences University of MontanaBiosciences Institute São Paulo State University-UNESP, Coastal CampusUniversity of San DiegoUniversidade Estadual Paulista (Unesp)Instituto Nacional de Pesquisas da Amazônia (INPA)University of Montana2021-06-25T10:22:57Z2021-06-25T10:22:57Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article493-502http://dx.doi.org/10.1007/s00360-021-01349-xJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, v. 191, n. 3, p. 493-502, 2021.0174-1578http://hdl.handle.net/11449/20588710.1007/s00360-021-01349-x2-s2.0-85100872631Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiologyinfo:eu-repo/semantics/openAccessGonzalez, R. J.Patrick, M. L.Duarte, R. M. [UNESP]Casciato, A.Thackeray, J.Day, N.Val, A. L.2025-04-11T19:38:34Zoai:repositorio.unesp.br:11449/205887Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-04-11T19:38:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum) |
| title |
Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum) |
| spellingShingle |
Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum) Gonzalez, R. J. Fish Ion regulation Low pH |
| title_short |
Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum) |
| title_full |
Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum) |
| title_fullStr |
Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum) |
| title_full_unstemmed |
Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum) |
| title_sort |
Exposure to pH 3.5 water has no effect on the gills of the Amazonian tambaqui (Colossoma macropomum) |
| dc.creator.none.fl_str_mv |
Gonzalez, R. J. Patrick, M. L. Duarte, R. M. [UNESP] Casciato, A. Thackeray, J. Day, N. Val, A. L. |
| author |
Gonzalez, R. J. |
| author_facet |
Gonzalez, R. J. Patrick, M. L. Duarte, R. M. [UNESP] Casciato, A. Thackeray, J. Day, N. Val, A. L. |
| author_role |
author |
| author2 |
Patrick, M. L. Duarte, R. M. [UNESP] Casciato, A. Thackeray, J. Day, N. Val, A. L. |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
University of San Diego Universidade Estadual Paulista (Unesp) Instituto Nacional de Pesquisas da Amazônia (INPA) University of Montana |
| dc.subject.por.fl_str_mv |
Fish Ion regulation Low pH |
| topic |
Fish Ion regulation Low pH |
| description |
Tambaqui (Colossoma macropomum) are a model species for tropical fish physiology, but details are lacking about their ionoregulatory response to acid waters. To provide specifics, we measured unidirectional Na+ fluxes in low pH waters. Sodium influx (JinNa) was uninhibited during acute exposure to pH 4.5 and 3.5, and Na efflux (JoutNa) rose only slightly at pH 3.5; net Na+ flux (JnetNa) remained positive at all pH. Similarly, during 24 h transfer to pH 3.5 JinNa, JoutNa, and JnetNa were unchanged at all times. Taking a closer look at the mechanism of Na+ transport in the gills of tambaqui we found that JinNa was uninhibited by HMA, a Na+/H+-exchanger blocker, and Benzamil, a Na+-channel inhibitor, casting doubt on their role in Na+ uptake in this fish. Measurement of Na+/K+-ATPase (NKA) and H+-ATPase (VHA) activity showed that neither changed at low pH compared to measurements at pH 6.5. Western blot analysis of ATPase expression saw no changes in amount of NKA and VHA at low pH, and immunohistochemistry showed expression of both NKA and VHA on lamellae and interlamellar region of tambaqui gills and that both proteins co-localized to the same gill cells. Location of expression also did not change in low pH water. Amazingly, tambaqui seem unaffected by pH 3.5 water, making them one of the most acid-tolerant fish species examined so far. In addition, they appear to share key ionoregulatory traits with other fish of the order Characiformes, which suggest a common origin for the ionoregulatory attributes. |
| publishDate |
2021 |
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2021-06-25T10:22:57Z 2021-06-25T10:22:57Z 2021-05-01 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://dx.doi.org/10.1007/s00360-021-01349-x Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, v. 191, n. 3, p. 493-502, 2021. 0174-1578 http://hdl.handle.net/11449/205887 10.1007/s00360-021-01349-x 2-s2.0-85100872631 |
| url |
http://dx.doi.org/10.1007/s00360-021-01349-x http://hdl.handle.net/11449/205887 |
| identifier_str_mv |
Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, v. 191, n. 3, p. 493-502, 2021. 0174-1578 10.1007/s00360-021-01349-x 2-s2.0-85100872631 |
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eng |
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eng |
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Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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493-502 |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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