In vivo killing of Staphylococcus aureus by toluidine blue-mediated photosensitization in an animal model wounds

The aims of this study were to determine whether S. aureus could be killed by toluidine blue-mediated photosensitization in vivo in an animal model. Twelve Wistar rats divided into three groups (n = 12): Group I: Control Group, the wounds were made and submitted to the application of the laser witho...

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Bibliographic Details
Authors: de Carvalho, Paulo de Tarso Camillo, Reis, Filipe Abdalla dos, Belchior, Ana Carulina Guimarães, Silva, Baldomero Antônio Kato da, Pereira, Daniel Martins, Bento, Nadia Cabral, Marques, Ana Paula da Costa, Oliveira, Geisa Poliane de
Format: article
Status:Published version
Publication Date:2009
Country:Brasil
Institution:Universidade Nove de Julho (UNINOVE)
Repository:Revista Conscientiae Saúde (Online)
Language:Portuguese
OAI Identifier:oai:ojs.periodicos.uninove.br:article/1415
Online Access:https://periodicos.uninove.br/saude/article/view/1415
Access Level:Open access
Keyword:Photodynamic therapy
Toluidine blue
Wound infection.
Description
Summary:The aims of this study were to determine whether S. aureus could be killed by toluidine blue-mediated photosensitization in vivo in an animal model. Twelve Wistar rats divided into three groups (n = 12): Group I: Control Group, the wounds were made and submitted to the application of the laser without the drug photosensitizing; Group II: The implementation of wounds received the toluidine blue, without application of laser; Group III: it was used toluidine blue, and application of laser-Indio phosphide Gallium-Aluminum (InGaAIP)  660nm power and density of 20 Joules/cm2. Statistical analysis of CFU by analysis of variance Kruskal-Wallis test shows significant intraoperative difference, photodynamic therapy group (p < 0, 05), the Dunns post hoc test shows significant difference between Group I when compared to Group II treated with LLLT (p < 0001). The results of this study show that photodynamic therapy with toluidine blue has reduced the number of Staphylococcus aureus in vivo.