Avaliação do metabolismo de carboidratos durante a maturação de frutos do meloeiro de variedades climatéricas e não-climatéricas
Cucumis melo L. popularly known as melon tree, belongs to the Cucurbitaceae family and is one of the most important crops, being one of the largest productions and exports in Brazil. This species has variable genotypes and phenotypes, with a wide diversity of cultivars. In Brazilian territory, the A...
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| Tipo de documento: | dissertação |
| Estado: | Versão publicada |
| Data de publicação: | 2023 |
| País: | Brasil |
| Recursos: | Universidade Federal do Ceará (UFC) |
| Repositório: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
| Idioma: | português |
| OAI Identifier: | oai:repositorio.ufc.br:riufc/81145 |
| Acesso em linha: | http://repositorio.ufc.br/handle/riufc/81145 |
| Access Level: | Acceso aberto |
| Palavra-chave: | CNPQ::CIENCIAS BIOLOGICAS Cucumis melo L. Metabolismo de carboidratos Amadurecimento Carbohydrate metabolism Ripening |
| Resumo: | Cucumis melo L. popularly known as melon tree, belongs to the Cucurbitaceae family and is one of the most important crops, being one of the largest productions and exports in Brazil. This species has variable genotypes and phenotypes, with a wide diversity of cultivars. In Brazilian territory, the A5 Inodorus and C1 Cantalupensis varieties stand out as being of greatest economic interest, presenting notable differences, especially in the ripening process of their fruits. The objective of this study was to evaluate carbohydrate metabolism during the ripening process of climacteric and non-climacteric melons of the A5 Inodorus and C1 Cantalupensis varieties. Six fruits were collected at different stages of their maturation, taking into account the days after pollination (DAP). A5 Inodorus melons were harvested at 24, 26, 28, 30, 32 and 34 DAP, while C1 Cantalupensis melons were harvested at 18, 20, 22, 24, 26 and 28 DAP. The fruits were subjected to analysis of carbohydrate metabolism enzymes, sugar profile by HPLC, osmotic potential and electrolyte leakage from tissues. The results indicated that in non- climacteric fruits of the A5 Inodorus variety, the maximum accumulation of sucrose occurred at 34 DAP. Analyzes related to cell turgidity and integrity indicated the impairment of the cell wall in the final stages of maturation, confirmed by the activity of β-GAL, which revealed changes in the cell wall. The performance of SPS played a central role in the accumulation of sucrose in this variety, and the activity of the enzymes SUS, IVN and IVA corroborated this finding. In the climatic fruits of the C1 Cantalupensis variety, there was no accumulation of sucrose, but the presence of glucose and fructose was observed. The results associated with turgidity and cell integrity indicated common behavior in climatic fruits. SPS and SUS increased during maturation, aiding in the production of glucose and fructose. Invertases developed for post-harvest loss of sucrose, and β-GAL show changes in the cell wall. In both varieties, carbohydrate metabolism enzymes have a direct influence on the maturation process. |
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