Reduced genetic variability within coding and non-coding regions of the echinococcus multilocularis genome

Echinococcus multilocularis, a vulpine intestinal tapeworm, is the causative agent of alveolar echinococosis in humans, one of the most severe and lethal parasitic infections in man. To date, there is very little knowledge about the genetical polymorphism of this parasite. To assess sequence polymor...

Descripción completa

Detalles Bibliográficos
Autores: Haag, Karen Luisa, Zaha, Arnaldo, Araujo, Aldo Mellender de, Gottstein, Bruno
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1997
País:Brasil
Institución:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/27470
Acceso en línea:http://hdl.handle.net/10183/27470
Access Level:acceso abierto
Palabra clave:Echinococcus multilocularis
Polimorfismo
Variação genética
SSCP
Strain
Genetic variability
id BR_d4555f4e604ee4e5ea482033c6f0dfec
oai_identifier_str oai:www.lume.ufrgs.br:10183/27470
network_acronym_str BR
network_name_str Brasil
repository_id_str
spelling 2011-01-22T05:59:05Z19970031-1820http://hdl.handle.net/10183/27470000156494Echinococcus multilocularis, a vulpine intestinal tapeworm, is the causative agent of alveolar echinococosis in humans, one of the most severe and lethal parasitic infections in man. To date, there is very little knowledge about the genetical polymorphism of this parasite. To assess sequence polymorphism, we analysed a sample of 33 E. multilocularis isolates from Europe, North America and Asia by PCR-SSCP followed by nucleotide sequencing. This assessment was performed comparatively to sheep, cattle and pig E. granulosus strains. Coding (nuclear antigen B and mitochondrial NADH dehydrogenase genes) and non-coding (introns of actin and homeobox-containing genes) regions of the parasite genome were chosen as targets. Since the estimated nucleotide diversity among genotypes of E. multilocularis were, in general, 10 times lower than among the recognized different strains of E. granulosus, we suggest that the conventional classi®cation of the former species in 2 separated strains (European and North American) should be reviewed.application/pdfengParasitology. Cambridge, Inglaterra. vol. 115, part 5 (nov. 1997), p. 521-529Echinococcus multilocularisPolimorfismoVariação genéticaEchinococcus multilocularisSSCPStrainGenetic variabilityReduced genetic variability within coding and non-coding regions of the echinococcus multilocularis genomeEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSHaag, Karen LuisaZaha, ArnaldoAraujo, Aldo Mellender deGottstein, BrunoORIGINAL000156494.pdf000156494.pdfTexto completo (inglês)application/pdf510028http://www.lume.ufrgs.br/bitstream/10183/27470/1/000156494.pdf6b74eaf62e3140b2ddb85c0d2e4f42ddMD51TEXT000156494.pdf.txt000156494.pdf.txtExtracted Texttext/plain31062http://www.lume.ufrgs.br/bitstream/10183/27470/2/000156494.pdf.txt36103d02b718ba1f0586a2e95ac31016MD52THUMBNAIL000156494.pdf.jpg000156494.pdf.jpgGenerated Thumbnailimage/jpeg1944http://www.lume.ufrgs.br/bitstream/10183/27470/3/000156494.pdf.jpgd3ef6a74f4358ae401090593e1cd44cfMD5310183/274702024-04-10 06:32:39.734109oai:www.lume.ufrgs.br:10183/27470Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2024-04-10T09:32:39Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Reduced genetic variability within coding and non-coding regions of the echinococcus multilocularis genome
title Reduced genetic variability within coding and non-coding regions of the echinococcus multilocularis genome
spellingShingle Reduced genetic variability within coding and non-coding regions of the echinococcus multilocularis genome
Haag, Karen Luisa
Echinococcus multilocularis
Polimorfismo
Variação genética
Echinococcus multilocularis
SSCP
Strain
Genetic variability
title_short Reduced genetic variability within coding and non-coding regions of the echinococcus multilocularis genome
title_full Reduced genetic variability within coding and non-coding regions of the echinococcus multilocularis genome
title_fullStr Reduced genetic variability within coding and non-coding regions of the echinococcus multilocularis genome
title_full_unstemmed Reduced genetic variability within coding and non-coding regions of the echinococcus multilocularis genome
title_sort Reduced genetic variability within coding and non-coding regions of the echinococcus multilocularis genome
dc.creator.none.fl_str_mv Haag, Karen Luisa
Zaha, Arnaldo
Araujo, Aldo Mellender de
Gottstein, Bruno
author Haag, Karen Luisa
author_facet Haag, Karen Luisa
Zaha, Arnaldo
Araujo, Aldo Mellender de
Gottstein, Bruno
author_role author
author2 Zaha, Arnaldo
Araujo, Aldo Mellender de
Gottstein, Bruno
author2_role author
author
author
dc.subject.por.fl_str_mv Echinococcus multilocularis
Polimorfismo
Variação genética
topic Echinococcus multilocularis
Polimorfismo
Variação genética
Echinococcus multilocularis
SSCP
Strain
Genetic variability
dc.subject.eng.fl_str_mv Echinococcus multilocularis
SSCP
Strain
Genetic variability
description Echinococcus multilocularis, a vulpine intestinal tapeworm, is the causative agent of alveolar echinococosis in humans, one of the most severe and lethal parasitic infections in man. To date, there is very little knowledge about the genetical polymorphism of this parasite. To assess sequence polymorphism, we analysed a sample of 33 E. multilocularis isolates from Europe, North America and Asia by PCR-SSCP followed by nucleotide sequencing. This assessment was performed comparatively to sheep, cattle and pig E. granulosus strains. Coding (nuclear antigen B and mitochondrial NADH dehydrogenase genes) and non-coding (introns of actin and homeobox-containing genes) regions of the parasite genome were chosen as targets. Since the estimated nucleotide diversity among genotypes of E. multilocularis were, in general, 10 times lower than among the recognized different strains of E. granulosus, we suggest that the conventional classi®cation of the former species in 2 separated strains (European and North American) should be reviewed.
publishDate 1997
dc.date.issued.fl_str_mv 1997
dc.date.accessioned.fl_str_mv 2011-01-22T05:59:05Z
dc.type.driver.fl_str_mv Estrangeiro
info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/27470
dc.identifier.issn.pt_BR.fl_str_mv 0031-1820
dc.identifier.nrb.pt_BR.fl_str_mv 000156494
identifier_str_mv 0031-1820
000156494
url http://hdl.handle.net/10183/27470
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Parasitology. Cambridge, Inglaterra. vol. 115, part 5 (nov. 1997), p. 521-529
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
bitstream.url.fl_str_mv http://www.lume.ufrgs.br/bitstream/10183/27470/1/000156494.pdf
http://www.lume.ufrgs.br/bitstream/10183/27470/2/000156494.pdf.txt
http://www.lume.ufrgs.br/bitstream/10183/27470/3/000156494.pdf.jpg
bitstream.checksum.fl_str_mv 6b74eaf62e3140b2ddb85c0d2e4f42dd
36103d02b718ba1f0586a2e95ac31016
d3ef6a74f4358ae401090593e1cd44cf
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)
repository.mail.fl_str_mv lume@ufrgs.br
_version_ 1853675729172037632
score 15,300724

Similares en LA Referencia