Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake

Objectives: To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland.Methods: Isolates presenting MICs of nalidixic acid or ciprofloxacin >= 1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids wer...

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Detalles Bibliográficos
Autores: Picão, Renata Cristina [UNIFESP], Poirel, Laurent, Demarta, Antonella, Ferreira Silva, Carla Sofia, Rita Corvaglia, Anna, Petrini, Orlando, Nordmann, Patrice
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/30979
Acceso en línea:http://dx.doi.org/10.1093/jac/dkn341
http://repositorio.unifesp.br/handle/11600/30979
Access Level:acceso abierto
Palabra clave:A. allosaccharophila
QnrS2
environment
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spelling Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lakeA. allosaccharophilaQnrS2environmentObjectives: To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland.Methods: Isolates presenting MICs of nalidixic acid or ciprofloxacin >= 1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids were transferred by transformation, and further analysis of the genetic structures surrounding the qnrS2 gene was carried out by PCR and sequencing.Results: A qnrS2 gene was identified from a single Aeromonas allosaccharophila isolate (Lugano lake, Lugano), as part of a mobile insertion cassette located on a broad host range IncU-type plasmid. This plasmid co-harboured a class 1 integron containing the aac(6 ')-Ib-cr, bla(OXA-1), catB3 and arr-3 gene cassettes.Conclusions: These findings strengthen further the role of Aeromonas spp. as a reservoir of antimicrobial resistance determinants in the environment.Hop Bicetre, AP HP, Serv Bacteriol Virol, INSERM Emerging Resistance Antibiot,U914,Fac Med, K Bicetre, FranceUniv Paris Sud, K Bicetre, FranceUniversidade Federal de São Paulo, Lab ALERTA, São Paulo, BrazilIst Cantonale Microbiol, Bellinzona, SwitzerlandUniv Geneva, Ctr Med & Univ, Geneva, SwitzerlandUniversidade Federal de São Paulo, Lab ALERTA, São Paulo, BrazilWeb of ScienceMinistere de l'Education Nationale et de la RechercheUniversite Paris XI, FranceSwiss National Science FoundationNational Research Program 'Antibiotic resistance'European CommunityCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)PDEEMinistere de l'Education Nationale et de la Recherche: UPRES-EA3539National Research Program 'Antibiotic resistance': NRP49European Community: DRESP2European Community: LSHM-CT-2005-018705PDEE: 3682/07-2Oxford Univ PressHop BicetreUniv Paris SudUniversidade Federal de São Paulo (UNIFESP)Ist Cantonale MicrobiolUniv Geneva2016-01-24T13:51:47Z2016-01-24T13:51:47Z2008-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion948-950http://dx.doi.org/10.1093/jac/dkn341Journal of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 62, n. 5, p. 948-950, 2008.10.1093/jac/dkn3410305-7453http://repositorio.unifesp.br/handle/11600/30979WOS:000259974700017ark:/48912/0013000023k7fengJournal of Antimicrobial Chemotherapyinfo:eu-repo/semantics/openAccesshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPPicão, Renata Cristina [UNIFESP]Poirel, LaurentDemarta, AntonellaFerreira Silva, Carla SofiaRita Corvaglia, AnnaPetrini, OrlandoNordmann, Patrice2016-01-24T11:51:47Zoai:repositorio.unifesp.br:11600/30979Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T11:51:47Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake
title Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake
spellingShingle Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake
Picão, Renata Cristina [UNIFESP]
A. allosaccharophila
QnrS2
environment
title_short Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake
title_full Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake
title_fullStr Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake
title_full_unstemmed Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake
title_sort Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake
dc.creator.none.fl_str_mv Picão, Renata Cristina [UNIFESP]
Poirel, Laurent
Demarta, Antonella
Ferreira Silva, Carla Sofia
Rita Corvaglia, Anna
Petrini, Orlando
Nordmann, Patrice
author Picão, Renata Cristina [UNIFESP]
author_facet Picão, Renata Cristina [UNIFESP]
Poirel, Laurent
Demarta, Antonella
Ferreira Silva, Carla Sofia
Rita Corvaglia, Anna
Petrini, Orlando
Nordmann, Patrice
author_role author
author2 Poirel, Laurent
Demarta, Antonella
Ferreira Silva, Carla Sofia
Rita Corvaglia, Anna
Petrini, Orlando
Nordmann, Patrice
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Hop Bicetre
Univ Paris Sud
Universidade Federal de São Paulo (UNIFESP)
Ist Cantonale Microbiol
Univ Geneva
dc.subject.por.fl_str_mv A. allosaccharophila
QnrS2
environment
topic A. allosaccharophila
QnrS2
environment
description Objectives: To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland.Methods: Isolates presenting MICs of nalidixic acid or ciprofloxacin >= 1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids were transferred by transformation, and further analysis of the genetic structures surrounding the qnrS2 gene was carried out by PCR and sequencing.Results: A qnrS2 gene was identified from a single Aeromonas allosaccharophila isolate (Lugano lake, Lugano), as part of a mobile insertion cassette located on a broad host range IncU-type plasmid. This plasmid co-harboured a class 1 integron containing the aac(6 ')-Ib-cr, bla(OXA-1), catB3 and arr-3 gene cassettes.Conclusions: These findings strengthen further the role of Aeromonas spp. as a reservoir of antimicrobial resistance determinants in the environment.
publishDate 2008
dc.date.none.fl_str_mv 2008-11-01
2016-01-24T13:51:47Z
2016-01-24T13:51:47Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1093/jac/dkn341
Journal of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 62, n. 5, p. 948-950, 2008.
10.1093/jac/dkn341
0305-7453
http://repositorio.unifesp.br/handle/11600/30979
WOS:000259974700017
dc.identifier.dark.fl_str_mv ark:/48912/0013000023k7f
url http://dx.doi.org/10.1093/jac/dkn341
http://repositorio.unifesp.br/handle/11600/30979
identifier_str_mv Journal of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 62, n. 5, p. 948-950, 2008.
10.1093/jac/dkn341
0305-7453
WOS:000259974700017
ark:/48912/0013000023k7f
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Antimicrobial Chemotherapy
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.format.none.fl_str_mv 948-950
dc.publisher.none.fl_str_mv Oxford Univ Press
publisher.none.fl_str_mv Oxford Univ Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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score 15.301629

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