Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae)

We investigated the use of Bacillus thuringiensis isolated in the state of Amazonas, in Brazil, for the biological control of the dengue vector Aedes aegypti. From 25 soil samples collected in nine municipalities, 484 bacterial colonies were obtained, 57 (11.78%) of which were identified as B. thuri...

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Bibliographic Details
Authors: Silva, Joelma Soares da, Pinheiro, Valéria Cristina Soares, Litaiff-Abreu, Eleilza, Polanczyk, Ricardo Antônio, Tadei, Wanderli Pedro
Format: article
Status:Published version
Publication Date:2015
Country:Brasil
Institution:Instituto Nacional de Pesquisas da Amazônia (INPA)
Repository:Repositório Institucional do INPA
Language:English
OAI Identifier:oai:repositorio:1/14756
Online Access:https://repositorio.inpa.gov.br/handle/1/14756
Access Level:Open access
Keyword:Aedes Aegypti
Bacillus Thuringiensis
Bacteria (microorganisms)
Culicidae
Diptera
Description
Summary:We investigated the use of Bacillus thuringiensis isolated in the state of Amazonas, in Brazil, for the biological control of the dengue vector Aedes aegypti. From 25 soil samples collected in nine municipalities, 484 bacterial colonies were obtained, 57 (11.78%) of which were identified as B. thuringiensis. Six isolates, IBt-03, IBt-06, IBt-07, IBt-28, IBt-30, and BtAM-27 showed insecticidal activity, and only BtAM-27 presents the five genes investigated cry4Aa, cry4Ba, cry10Aa, cry11Aa, and cry11Ba. The IBt-07 and IBt- 28, with lower LC50 values, showed equal toxicity compared to the standards. The isolates of B. thuringiensis from Amazonas constitute potential new means of biological control for A. aegypti, because of their larvicidal activity and the possibility that they may also contain new combinations of toxins. © 2015 Sociedade Brasileira de Entomologia. Published by Elsevier Editora Ltda.