Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms

BACKGROUND: Organoid cultivation in suspension culture requires agitation at low shear stress to allow for nutrient diffusion, which preserves tissue structure. Multiplex systems for organoid cultivation have been proposed, but whether they meet similar shear stress parameters as the regularly used...

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Autores: Goto-Silva, Livia, Ayad, Nadia M. E., Herzog, Iasmin L., Silva, Nilton P., Lamien, Bernard, Orlande, Helcio R. B., Souza, Annie da Costa, Ribeiro, Sidarta Tollendal Gomes, Martins, Michele, Domont, Gilberto B., Junqueira, Magno, Tovar-Moll, Fernanda, Rehen, Stevens K.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Institución:Universidade Federal do Rio Grande do Norte (UFRN)
Repositorio:Repositório Institucional da UFRN
Idioma:inglés
OAI Identifier:oai:repositorio.ufrn.br:123456789/26787
Acceso en línea:https://repositorio.ufrn.br/jspui/handle/123456789/26787
Access Level:acceso abierto
Palabra clave:Organoid cultures
brain organoids
computational fluid dynamics
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spelling Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platformsOrganoid culturesbrain organoidscomputational fluid dynamicsBACKGROUND: Organoid cultivation in suspension culture requires agitation at low shear stress to allow for nutrient diffusion, which preserves tissue structure. Multiplex systems for organoid cultivation have been proposed, but whether they meet similar shear stress parameters as the regularly used spinner flask and its correlation with the successful generation of brain organoids has not been determined. RESULTS: Here we used computational fluid dynamics (CFD) to simulate two multiplex culture conditions: steering plates on an orbital shaker and the use of a previously described bioreactor. The bioreactor had low speed and high shear stress regions that may affect cell aggregate growth, depending on volume, whereas the computed variables of the steering plates were closer to those of the spinning flask. CONCLUSION: Our protocol improves the initial steps of the standard brain organoid formation, and the produced organoids displayed regionalized brain structures, including retinal pigmented cells. Overall, we conclude that suspension culture on orbital steering plates is a cost-effective practical alternative to previously described platforms for the cultivation of brain organoids for research and multiplex testing.2019-03-15T13:08:42Z2019-03-15T13:08:42Z2019-03-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGOTO-SILVA, L. et al. Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms. BMC Dev Biol., v. 19, n. 1, p. 3, mar. 2019. doi: 10.1186/s12861-019-0183-yhttps://repositorio.ufrn.br/jspui/handle/123456789/2678710.1186/s12861-019-0183-yark:/41046/001300001zdgtengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessGoto-Silva, LiviaAyad, Nadia M. E.Herzog, Iasmin L.Silva, Nilton P.Lamien, BernardOrlande, Helcio R. B.Souza, Annie da CostaRibeiro, Sidarta Tollendal GomesMartins, MicheleDomont, Gilberto B.Junqueira, MagnoTovar-Moll, FernandaRehen, Stevens K.2025-05-29T17:20:05Zoai:repositorio.ufrn.br:123456789/26787Repositório InstitucionalPUBhttp://repositorio.ufrn.br/oai/repositorio@bczm.ufrn.bropendoar:2025-05-29T17:20:05Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.none.fl_str_mv Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms
title Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms
spellingShingle Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms
Goto-Silva, Livia
Organoid cultures
brain organoids
computational fluid dynamics
title_short Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms
title_full Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms
title_fullStr Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms
title_full_unstemmed Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms
title_sort Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms
dc.creator.none.fl_str_mv Goto-Silva, Livia
Ayad, Nadia M. E.
Herzog, Iasmin L.
Silva, Nilton P.
Lamien, Bernard
Orlande, Helcio R. B.
Souza, Annie da Costa
Ribeiro, Sidarta Tollendal Gomes
Martins, Michele
Domont, Gilberto B.
Junqueira, Magno
Tovar-Moll, Fernanda
Rehen, Stevens K.
author Goto-Silva, Livia
author_facet Goto-Silva, Livia
Ayad, Nadia M. E.
Herzog, Iasmin L.
Silva, Nilton P.
Lamien, Bernard
Orlande, Helcio R. B.
Souza, Annie da Costa
Ribeiro, Sidarta Tollendal Gomes
Martins, Michele
Domont, Gilberto B.
Junqueira, Magno
Tovar-Moll, Fernanda
Rehen, Stevens K.
author_role author
author2 Ayad, Nadia M. E.
Herzog, Iasmin L.
Silva, Nilton P.
Lamien, Bernard
Orlande, Helcio R. B.
Souza, Annie da Costa
Ribeiro, Sidarta Tollendal Gomes
Martins, Michele
Domont, Gilberto B.
Junqueira, Magno
Tovar-Moll, Fernanda
Rehen, Stevens K.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Organoid cultures
brain organoids
computational fluid dynamics
topic Organoid cultures
brain organoids
computational fluid dynamics
description BACKGROUND: Organoid cultivation in suspension culture requires agitation at low shear stress to allow for nutrient diffusion, which preserves tissue structure. Multiplex systems for organoid cultivation have been proposed, but whether they meet similar shear stress parameters as the regularly used spinner flask and its correlation with the successful generation of brain organoids has not been determined. RESULTS: Here we used computational fluid dynamics (CFD) to simulate two multiplex culture conditions: steering plates on an orbital shaker and the use of a previously described bioreactor. The bioreactor had low speed and high shear stress regions that may affect cell aggregate growth, depending on volume, whereas the computed variables of the steering plates were closer to those of the spinning flask. CONCLUSION: Our protocol improves the initial steps of the standard brain organoid formation, and the produced organoids displayed regionalized brain structures, including retinal pigmented cells. Overall, we conclude that suspension culture on orbital steering plates is a cost-effective practical alternative to previously described platforms for the cultivation of brain organoids for research and multiplex testing.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-15T13:08:42Z
2019-03-15T13:08:42Z
2019-03-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv GOTO-SILVA, L. et al. Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms. BMC Dev Biol., v. 19, n. 1, p. 3, mar. 2019. doi: 10.1186/s12861-019-0183-y
https://repositorio.ufrn.br/jspui/handle/123456789/26787
10.1186/s12861-019-0183-y
dc.identifier.dark.fl_str_mv ark:/41046/001300001zdgt
identifier_str_mv GOTO-SILVA, L. et al. Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms. BMC Dev Biol., v. 19, n. 1, p. 3, mar. 2019. doi: 10.1186/s12861-019-0183-y
10.1186/s12861-019-0183-y
ark:/41046/001300001zdgt
url https://repositorio.ufrn.br/jspui/handle/123456789/26787
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
repository.name.fl_str_mv Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)
repository.mail.fl_str_mv repositorio@bczm.ufrn.br
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score 15,300719

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