The antioxidant peptide salamandrin-i: First bioactive peptide identified from skin secretion of salamandra genus (salamandra salamandra)

Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, skin secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC and de novo sequenced using MALDI-TOF MS/MS. Next, we used an in silico platform...

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Detalles Bibliográficos
Autores: Plácido, Alexandra, Bueno, João, Barbosa, Eder A., Moreira, Daniel C., Dias, Jhones Do Nascimento, Cabral, Wanessa Felix, Albuquerque, Patrícia, Bessa, Lucinda J., Freitas, Jaime, Kuckelhaus, Selma A. S., Lima, Filipe C. D. A., Batagin-Neto, Augusto [UNESP], Brand, Guilherme D., Relvas, João B., Leite, José Roberto S. A., Eaton, Peter
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/200233
Acceso en línea:http://dx.doi.org/10.3390/biom10040512
http://hdl.handle.net/11449/200233
Access Level:acceso abierto
Palabra clave:Antioxidant peptides
Bioactive molecules
Portuguese biodiversity
Salamandra salamandra
Descripción
Sumario:Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, skin secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC and de novo sequenced using MALDI-TOF MS/MS. Next, we used an in silico platform to screen antioxidant molecules in the framework of density functional theory. One of the identified peptides, salamandrin-I, [M + H]+ = 1406.6 Da, was selected for solid-phase synthesis; it showed free radical scavenging activity against DPPH and ABTS radicals. Salamandrin-I did not show antimicrobial activity against Gram-positive and-negative bacteria. In vitro assays using human microglia and red blood cells showed that salamandrin-I has no cytotoxicity up to the concentration of 100 µM. In addition, in vivo toxicity tests on Galleria mellonella larvae resulted in no mortality at 20 and 40 mg/kg. Antioxidant peptides derived from natural sources are increasingly attracting interest. Among several applications, these peptides, such as salamandrin-I, can be used as templates in the design of novel antioxidant molecules that may contribute to devising strategies for more effective control of neurological disease.

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