Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp.

Symbiosis with microorganisms has been well documented for many marine invertebrate taxa. However, knowledge of the diversity of microorganisms associated with ascidians is still limited. This study assessed the microbial epibionts of Didemnum galacteum and Cystodytes sp., two ascidian species colle...

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Detalles Bibliográficos
Autores: Oliveira, Francisca Andréa da Silva, Colares, Geórgia B., Hissa, Denise Cavalcante, Angelim, Alysson Lira, Melo, Vânia M. M., Lotufo, Tito M. C.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Institución:Universidade Federal do Ceará (UFC)
Repositorio:Repositório Institucional da Universidade Federal do Ceará (UFC)
Idioma:portugués
OAI Identifier:oai:repositorio.ufc.br:riufc/62936
Acceso en línea:http://www.repositorio.ufc.br/handle/riufc/62936
Access Level:acceso abierto
Palabra clave:Symbiosis
Ascidiacea
Microbiota
Brazil
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network_name_str Brasil
repository_id_str
dc.title.pt_BR.fl_str_mv Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp.
title Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp.
spellingShingle Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp.
Oliveira, Francisca Andréa da Silva
Symbiosis
Ascidiacea
Microbiota
Brazil
title_short Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp.
title_full Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp.
title_fullStr Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp.
title_full_unstemmed Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp.
title_sort Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp.
dc.creator.none.fl_str_mv Oliveira, Francisca Andréa da Silva
Colares, Geórgia B.
Hissa, Denise Cavalcante
Angelim, Alysson Lira
Melo, Vânia M. M.
Lotufo, Tito M. C.
author Oliveira, Francisca Andréa da Silva
author_facet Oliveira, Francisca Andréa da Silva
Colares, Geórgia B.
Hissa, Denise Cavalcante
Angelim, Alysson Lira
Melo, Vânia M. M.
Lotufo, Tito M. C.
author_role author
author2 Colares, Geórgia B.
Hissa, Denise Cavalcante
Angelim, Alysson Lira
Melo, Vânia M. M.
Lotufo, Tito M. C.
author2_role author
author
author
author
author
dc.subject.por.fl_str_mv Symbiosis
Ascidiacea
Microbiota
Brazil
topic Symbiosis
Ascidiacea
Microbiota
Brazil
description Symbiosis with microorganisms has been well documented for many marine invertebrate taxa. However, knowledge of the diversity of microorganisms associated with ascidians is still limited. This study assessed the microbial epibionts of Didemnum galacteum and Cystodytes sp., two ascidian species collected from the western coast of Ceará state (Brazil), at Dois Coqueiros beach and the port of Pecém, respectively. The microbiota were examined using optical microscopy, followed by subsequent analysis of fingerprinting profiles obtained by denaturing gradient gel electrophoresis (DGGE) and 16S rRNA clone libraries. The microscopy analysis showed for both ascidians a community comprising cyanobacteria, mainly Prochloron-like species, and diatoms. The DGGE results indicated that D. galacteum hosts a more diverse microbiota when compared to Cystodytes sp. The same analysis also suggested that the diversity of the seawater microbiota was higher than the diversity of the ascidian-associated microbiota. The analysis of the 16S rRNA clone libraries revealed the dominance of Proteobacteria symbionts associated with both ascidians, with Alphaproteobacteria as the major component in D. galacteum and Gammaproteobacteria the major component in Cystodytes sp. The analysis of the clone libraries also revealed the presence of other taxa such as Bacteroidetes, Planctomycetes, Actinobacteria, Cyanobacteria, and uncultured bacteria in D. galacteum, but not in Cystodytes sp. Among the bacteria found to be exclusively associated with the ascidians, none were shared by the two studied hosts. The combined results point to a diverse microbiota associated with the external surface of the ascidians, with a mixed composition including organisms typically found in the surrounding seawater, but also a more specific set of taxa
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2021-12-13T13:24:28Z
dc.date.available.fl_str_mv 2021-12-13T13:24:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Francisca Andréa da Silva et al. Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp. Symbiosis, [s. l.], v. 59, p. 57-63, 2013.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/62936
identifier_str_mv OLIVEIRA, Francisca Andréa da Silva et al. Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp. Symbiosis, [s. l.], v. 59, p. 57-63, 2013.
url http://www.repositorio.ufc.br/handle/riufc/62936
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Symbiosis
publisher.none.fl_str_mv Symbiosis
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
bitstream.url.fl_str_mv http://repositorio.ufc.br/bitstream/riufc/62936/2/license.txt
http://repositorio.ufc.br/bitstream/riufc/62936/1/2013_art_fasoliveira.pdf
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repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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spelling 2021-12-13T13:24:28Z2021-12-13T13:24:28Z2013OLIVEIRA, Francisca Andréa da Silva et al. Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp. Symbiosis, [s. l.], v. 59, p. 57-63, 2013.http://www.repositorio.ufc.br/handle/riufc/62936SymbiosisSymbiosisAscidiaceaMicrobiotaBrazilMicrobial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSymbiosis with microorganisms has been well documented for many marine invertebrate taxa. However, knowledge of the diversity of microorganisms associated with ascidians is still limited. This study assessed the microbial epibionts of Didemnum galacteum and Cystodytes sp., two ascidian species collected from the western coast of Ceará state (Brazil), at Dois Coqueiros beach and the port of Pecém, respectively. The microbiota were examined using optical microscopy, followed by subsequent analysis of fingerprinting profiles obtained by denaturing gradient gel electrophoresis (DGGE) and 16S rRNA clone libraries. The microscopy analysis showed for both ascidians a community comprising cyanobacteria, mainly Prochloron-like species, and diatoms. The DGGE results indicated that D. galacteum hosts a more diverse microbiota when compared to Cystodytes sp. The same analysis also suggested that the diversity of the seawater microbiota was higher than the diversity of the ascidian-associated microbiota. The analysis of the 16S rRNA clone libraries revealed the dominance of Proteobacteria symbionts associated with both ascidians, with Alphaproteobacteria as the major component in D. galacteum and Gammaproteobacteria the major component in Cystodytes sp. The analysis of the clone libraries also revealed the presence of other taxa such as Bacteroidetes, Planctomycetes, Actinobacteria, Cyanobacteria, and uncultured bacteria in D. galacteum, but not in Cystodytes sp. Among the bacteria found to be exclusively associated with the ascidians, none were shared by the two studied hosts. The combined results point to a diverse microbiota associated with the external surface of the ascidians, with a mixed composition including organisms typically found in the surrounding seawater, but also a more specific set of taxainfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCOliveira, Francisca Andréa da SilvaColares, Geórgia B.Hissa, Denise CavalcanteAngelim, Alysson LiraMelo, Vânia M. M.Lotufo, Tito M. 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