Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans

Candida infection is an important cause of morbidity and mortality in immunocompromised patients. The increase in its incidence has been associated with resistance to antimicrobial therapy and biofilm formation. The aim of this study was to evaluate the efficacy of tea tree oil (TTO) and its main co...

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Bibliographic Details
Authors: Francisconi, Renata Serignoli [UNESP], Huacho, Patricia Milagros Maquera [UNESP], Tonon, Caroline Coradi [UNESP], Bordini, Ester Alves Ferreira [UNESP], Correia, Marília Ferreira [UNESP], Sardi, Janaína de Cássia Orlandi, Spolidorio, Denise Madalena Palomari [UNESP]
Format: article
Status:Published version
Publication Date:2020
Country:Brasil
Institution:Universidade Estadual Paulista (UNESP)
Repository:Repositório Institucional da UNESP
Language:English
OAI Identifier:oai:repositorio.unesp.br:11449/200645
Online Access:http://dx.doi.org/10.1590/1807-3107BOR-2020.VOL34.0050
http://hdl.handle.net/11449/200645
Access Level:Open access
Keyword:Biofilms
Candida albicans
Mycoses
Phytotherapy
Description
Summary:Candida infection is an important cause of morbidity and mortality in immunocompromised patients. The increase in its incidence has been associated with resistance to antimicrobial therapy and biofilm formation. The aim of this study was to evaluate the efficacy of tea tree oil (TTO) and its main component-terpinen-4-ol-against resistant Candida albicans strains (genotypes A and B) identified by molecular typing and against C. albicans ATCC 90028 and SC 5314 reference strains in planktonic and biofilm cultures. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity. Results were obtained from analysis of the biofilm using the cell proliferation assay 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2Htetrazolium-5-carboxanilide (XTT) and confocal laser scanning microscopy (CLSM). Terpinen-4-ol and TTO inhibited C. albicans growth. CLSM confirmed that 17.92 mg/mL of TTO and 8.86 mg/mL of terpinen-4-ol applied for 60 s (rinse simulation) interfered with biofilm formation. Hence, this in vitro s tudy r evealed t hat n atural substances such as TTO and terpinen-4-ol present promising results for the treatment of oral candidiasis.

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