IDENTIFICATION OF WILD MAMMALS FROM PANTANAL SUL-MATOGROSSENSE CARRIERS OF Leptospira spp.

A survey of Leptospira spp. in wild mammals from the southern Pantanal of Mato Grosso do Sul was performed by microscopic agglutination test (MAT) and polymerase chain reaction (PCR). The serovars most frequently found were Hardjobovis (28%), Icterohemorhagiae (12%), M110/2006 strain (isolated from...

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Detalhes bibliográficos
Autores: Vieira, Anahi Souto, Rosinha, Grácia Maria Soares, Vasconcellos, Silvio Arruda, Moaris, Zenaide Maria de, Viana, Rosielle Campozano, Oliveira, Carina Elisei de, Soares, Cleber Oliveira, Araújo, Flabio Ribeiro de, Mourão, Guilherme Miranda, Bianchi, Rita de Cassia, Olifiers, Natalie, Rademaker, Vitor, Rocha, Fabiana Lopes, Pellegrin, Aiesca Oliveira
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2013
País:Brasil
Recursos:Universidade Federal de Goiás (UFG)
Repositório:Ciência animal brasileira (Online)
Idioma:português
OAI Identifier:oai:ojs.revistas.ufg.br:article/17147
Acesso em linha:https://revistas.ufg.br/vet/article/view/17147
Access Level:Acceso aberto
Palavra-chave:Animal sanity
Sanidade Animal
Descrição
Resumo:A survey of Leptospira spp. in wild mammals from the southern Pantanal of Mato Grosso do Sul was performed by microscopic agglutination test (MAT) and polymerase chain reaction (PCR). The serovars most frequently found were Hardjobovis (28%), Icterohemorhagiae (12%), M110/2006 strain (isolated from Cerdocyon thous, 16%), Canicola (L014 isolated from Bos Taurus, 4%), Whitcombi (4%), Pomona (20%), Autumnalis (12%) and Copenhageni (M9/99 isolated from Rattus norvegicus, 4%). From the 79 samples tested by PCR, 21 (26.58%) were positive, resulting in the amplification fragment of approximately 331pb. Two amplified fragments from C. thous were cloned, sequenced and identified as L. interrogans by phylogenetic analysis.KEYWORDS: leptospirosis; Pantanal; PCR; serology; wild mammals.

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