ESTUDO COMPARATIVO ENTRE OS TESTES ADOTADOS PELO PNCEBT PARA O DIAGNÓSTICO SOROLÓGICO DA BRUCELOSE EM BOVINOS*
Serum samples from 1,061 bovine were analyzed by serological diagnostic techniques adopted by the Brazilian Program for Animal Brucellosis and Tuberculosis Control and Eradication in order to compare, as a screening test, the rose Bengal plate test (RBPT), and as confirmatory tests, the 2-mercaptoet...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.unesp.br:11449/211985 |
| Acceso en línea: | http://dx.doi.org/10.1590/1808-1657v77p0112010 http://hdl.handle.net/11449/211985 |
| Access Level: | acceso abierto |
| Palabra clave: | Bovine brucellosis serodiagnosis agreement sensitivity specificity Brucelose bovina diagnóstico sorológico concordância sensibilidade diagnóstica especificidade diagnóstica |
| Sumario: | Serum samples from 1,061 bovine were analyzed by serological diagnostic techniques adopted by the Brazilian Program for Animal Brucellosis and Tuberculosis Control and Eradication in order to compare, as a screening test, the rose Bengal plate test (RBPT), and as confirmatory tests, the 2-mercaptoethanol plus standard tube agglutination test (2-ME), and the complement fixation test (CFT). Relative sensitivity of 99.6%, 98.8% and 91.1% were observed, respectively, for RBPT, 2-ME and CFT, and relative specificity of 83.9%, 96.2% and 100.0%. The agreement between tests was analyzed by the kappa statistic (2-ME cut-off = titer 25, and CFT cutoff = titer 4). RBPT showed a substantial agreement (kappa: 2-ME = 0.80; and RFC = 0.73) with the confirmatory tests, and excellent agreement between the confirmatory tests (kappa = 0.86) was observed. However sera with a negative result in one of the confirmatory tests and a high titer in the other were observed, reinforcing that the serological diagnosis of bovine brucellosis is more reliable when based on the results of several tests. |
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