Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigs

In the current study we have performed two experiments to evaluate the effects of adipogenic induction media on dedifferentiated fat (DFAT) cells redifferentiation into mature adipocytes. In experiment 1, we aimed to evaluate whether it is necessary to use insulin in the induction media to allow DFA...

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Detalles Bibliográficos
Autores: Bueno, R., Campos, C. F. de, Veroneze, R., Silva, W., Sanglard, L. M. P., Alcantara, L., Serão, N. V. L., Hausman, G. J., Dodson, M. V., Duarte, M. S., Guimarães, S. E. F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Universidade Federal de Viçosa (UFV)
Repositorio:LOCUS Repositório Institucional da UFV
Idioma:inglés
OAI Identifier:oai:locus.ufv.br:123456789/23081
Acceso en línea:https://doi.org/10.1016/j.livsci.2017.03.009
http://www.locus.ufv.br/handle/123456789/23081
Access Level:acceso abierto
Palabra clave:Adipogenesis
Adipocyte
IBMX
Insulin
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spelling Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigsAdipogenesisAdipocyteIBMXInsulinIn the current study we have performed two experiments to evaluate the effects of adipogenic induction media on dedifferentiated fat (DFAT) cells redifferentiation into mature adipocytes. In experiment 1, we aimed to evaluate whether it is necessary to use insulin in the induction media to allow DFAT cells differentiation into mature adipocytes by establishing two experimental treatments where insulin was either withdrawn from the culture medium, after 72 h of a normal induction period (Treatment 1: Insulin-) or kept in culture media (Treatment 2: Insulin+) for 16 d. In experiment 2, we aimed to evaluate if the lack of 3-isobutyl-1-methylxanthine (IBMX) in the induction medium would affect the differentiation of DFAT cells into mature adipocytes. For that, DFAT cells were induced to differentiate into lipid assimilating adipocytes using an induction medium containing IBMX (Treatment 1: IBMX+) or without IBMX (Treatment 2: IBMX-) during the first 72 h of induction. In both experiments we have evaluated the mRNA expression of lipid metabolism markers and cell morphology through Oil-Red staining as indicators of differentiation of DFAT cells into lipid-assimilating cells. The results of Experiment 1 revealed no differences in mRNA expression for any of the lipid metabolism markers with exception of GLUT4 (P=0.02), which was greater in Insulin- compared to Insulin+ treatment. Similarly, no differences were observed for mRNA expression of adipogenic markers between IBMX+ and IBMX- treatments with exception of FABP4 (P=0.01), which was greater for the IBMX- compared to IBMX+ treatment. In both experiments we did not observed any differences in cell morphology among treatments. Our results suggest that neither insulin nor IBMX are required to accelerate redifferentiation process of pig-derived DFAT cells.Livestock Science2019-01-17T16:46:04Z2019-01-17T16:46:04Z2017-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1871-1413https://doi.org/10.1016/j.livsci.2017.03.009http://www.locus.ufv.br/handle/123456789/23081engVolume 199, Pages 57- 62, May 20172017 Elsevier B.V. All rights reserved.info:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVBueno, R.Campos, C. F. deVeroneze, R.Silva, W.Sanglard, L. M. P.Alcantara, L.Serão, N. V. L.Hausman, G. J.Dodson, M. V.Duarte, M. S.Guimarães, S. E. F.2024-07-12T08:43:15Zoai:locus.ufv.br:123456789/23081Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T08:43:15LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigs
title Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigs
spellingShingle Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigs
Bueno, R.
Adipogenesis
Adipocyte
IBMX
Insulin
title_short Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigs
title_full Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigs
title_fullStr Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigs
title_full_unstemmed Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigs
title_sort Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigs
dc.creator.none.fl_str_mv Bueno, R.
Campos, C. F. de
Veroneze, R.
Silva, W.
Sanglard, L. M. P.
Alcantara, L.
Serão, N. V. L.
Hausman, G. J.
Dodson, M. V.
Duarte, M. S.
Guimarães, S. E. F.
author Bueno, R.
author_facet Bueno, R.
Campos, C. F. de
Veroneze, R.
Silva, W.
Sanglard, L. M. P.
Alcantara, L.
Serão, N. V. L.
Hausman, G. J.
Dodson, M. V.
Duarte, M. S.
Guimarães, S. E. F.
author_role author
author2 Campos, C. F. de
Veroneze, R.
Silva, W.
Sanglard, L. M. P.
Alcantara, L.
Serão, N. V. L.
Hausman, G. J.
Dodson, M. V.
Duarte, M. S.
Guimarães, S. E. F.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Adipogenesis
Adipocyte
IBMX
Insulin
topic Adipogenesis
Adipocyte
IBMX
Insulin
description In the current study we have performed two experiments to evaluate the effects of adipogenic induction media on dedifferentiated fat (DFAT) cells redifferentiation into mature adipocytes. In experiment 1, we aimed to evaluate whether it is necessary to use insulin in the induction media to allow DFAT cells differentiation into mature adipocytes by establishing two experimental treatments where insulin was either withdrawn from the culture medium, after 72 h of a normal induction period (Treatment 1: Insulin-) or kept in culture media (Treatment 2: Insulin+) for 16 d. In experiment 2, we aimed to evaluate if the lack of 3-isobutyl-1-methylxanthine (IBMX) in the induction medium would affect the differentiation of DFAT cells into mature adipocytes. For that, DFAT cells were induced to differentiate into lipid assimilating adipocytes using an induction medium containing IBMX (Treatment 1: IBMX+) or without IBMX (Treatment 2: IBMX-) during the first 72 h of induction. In both experiments we have evaluated the mRNA expression of lipid metabolism markers and cell morphology through Oil-Red staining as indicators of differentiation of DFAT cells into lipid-assimilating cells. The results of Experiment 1 revealed no differences in mRNA expression for any of the lipid metabolism markers with exception of GLUT4 (P=0.02), which was greater in Insulin- compared to Insulin+ treatment. Similarly, no differences were observed for mRNA expression of adipogenic markers between IBMX+ and IBMX- treatments with exception of FABP4 (P=0.01), which was greater for the IBMX- compared to IBMX+ treatment. In both experiments we did not observed any differences in cell morphology among treatments. Our results suggest that neither insulin nor IBMX are required to accelerate redifferentiation process of pig-derived DFAT cells.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
2019-01-17T16:46:04Z
2019-01-17T16:46:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1871-1413
https://doi.org/10.1016/j.livsci.2017.03.009
http://www.locus.ufv.br/handle/123456789/23081
identifier_str_mv 1871-1413
url https://doi.org/10.1016/j.livsci.2017.03.009
http://www.locus.ufv.br/handle/123456789/23081
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Volume 199, Pages 57- 62, May 2017
dc.rights.driver.fl_str_mv 2017 Elsevier B.V. All rights reserved.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv 2017 Elsevier B.V. All rights reserved.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Livestock Science
publisher.none.fl_str_mv Livestock Science
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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score 15.301603

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