In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms
Aim: To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT). Methods: The microorganism indicators were: Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (108 cells/mL), from which aliquots were transferred...
| Autores: | , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Brasil |
| Recursos: | Universidade Estadual de Campinas (UNICAMP) |
| Repositorio: | Brazilian journal of oral sciences (Online) |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.periodicos.sbu.unicamp.br:article/8640933 |
| Acesso em linha: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640933 |
| Access Level: | acceso abierto |
| Palavra-chave: | Endodontics Enterococcus faecalis Methylene blue Microbiology Photodynamic therapy Odontologia |
| Resumo: | Aim: To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT). Methods: The microorganism indicators were: Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (108 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane’s comparisons. Results:The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001). Conclusions: PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus. |
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