Non-enzymatic impedimetric sensor based on 3-aminophenylboronic acid functionalized screen-printed carbon electrode for highly sensitive glucose detection

A highly sensitive glucose sensor was prepared by a one-step method using 3-aminophenyl boronic acid as a unit of recognition and a screen-printed carbon electrode (SPCE) as an electrochemical transducer. Scanning Electron Microscopy confirmed the success of the functionalization of the SPCE due to...

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Bibliographic Details
Authors: Ricardo Adriano Dorledo de Faria, Hassan Iden, Luiz Guilherme Dias Heneine, Tulio Matencio, Younès Messaddeq
Format: article
Status:Published version
Publication Date:2019
Country:Brasil
Institution:Universidade Federal de Minas Gerais (UFMG)
Repository:Repositório Institucional da UFMG
Language:English
OAI Identifier:oai:repositorio.ufmg.br:1843/57518
Online Access:https://doi.org/10.3390/s19071686
http://hdl.handle.net/1843/57518
https://orcid.org/0000-0002-7025-7042
https://orcid.org/0000-0003-0707-2691
https://orcid.org/0000-0002-5660-8125
https://orcid.org/0000-0002-0868-2726
Access Level:Open access
Keyword:Sensor
Glucose
Electrochemical impedance spectroscopy
Boronic acid
Eletroquímica
Detectores eletroquímicos
Espectroscopia de impedância
Glicose
Description
Summary:A highly sensitive glucose sensor was prepared by a one-step method using 3-aminophenyl boronic acid as a unit of recognition and a screen-printed carbon electrode (SPCE) as an electrochemical transducer. Scanning Electron Microscopy confirmed the success of the functionalization of the SPCE due to the presence of clusters of boronic acid distributed on the carbon surface. In agreement with the Electrochemical Impedance Spectroscopy (EIS) tests performed before and after the functionalization, Cyclic Voltammetry results indicated that the electroactivity of the electrode decreased 37.9% owing to the presence of the poly phenylboronic acid on the electrode surface. EIS revealed that the sensor was capable to selectively detect glucose at a broad range of concentrations (limit of detection of 8.53 × 10−9 M), not recognizing fructose and sucrose. The device presented a stable impedimetric response when immediately prepared but suffered the influence of the storage time and some interfering species (dopamine, NaCl and animal serum). The response time at optimized conditions was estimated to be equal to 4.0 ± 0.6 s.