Contribuição para o conhecimento fitoquímico da Vismia guianensis (Hypericaceae)

Vismia guianensis (Hypericaceae) is popularly known in Brazil as lacre or goma-lacre. In the present work the extraction of chemicals from the leaves of V. guianensis by maceration with ethanol. The phytochemical screening showed the presence of flavonoids, saponins, steroids, tannins and anthraquin...

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Bibliographic Details
Author: Teles, Maria Madalena Rocha Silva
Format: master thesis
Status:Published version
Publication Date:2014
Country:Brasil
Institution:Universidade Federal da Paraíba (UFPB)
Repository:Biblioteca Digital de Teses e Dissertações da UFPB
Language:Portuguese
OAI Identifier:oai:repositorio.ufpb.br:tede/6796
Online Access:https://repositorio.ufpb.br/jspui/handle/tede/6796
Access Level:Open access
Keyword:Vismia guianensis
Vismiaquinona A
Canferol
Óleo essencial
Atividade antimicrobiana
Vismiaquinone A
Kaempferol
Essential oil
Antimicrobial activity
CIENCIAS BIOLOGICAS::FARMACOLOGIA
Description
Summary:Vismia guianensis (Hypericaceae) is popularly known in Brazil as lacre or goma-lacre. In the present work the extraction of chemicals from the leaves of V. guianensis by maceration with ethanol. The phytochemical screening showed the presence of flavonoids, saponins, steroids, tannins and anthraquinones. By column chromatography were isolated Stigmasterol, β-Sitosterol and Vismiaquinone A from the hexane phase, and Kaempferol from the ethyl acetate phase. The structures were elucidated using nuclear magnetic resonance 1H and 13C unidimensional and bidimensional. Of the inflorescences of V. guianensis, was performed extraction of essential oil by steam drag in Clevenger apparatus, and parallel soxhlet extraction with hexane, ethyl acetate and methanol solvents. The determination of the constituents of the essential oil was performed by gas chromatography coupled to a mass spectrometer (GC/MS) and antimicrobial activity was evaluated against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and was determined by minimum inhibitory concentration (MIC), activity was considered significant in these bacterial cultures