Morphological studies in a model for dengue-2 virus infection in mice

Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.

Detalles Bibliográficos
Autores: Barth, Ortrud Monika, Barreto, Débora Ferreira, Paes, Marciano Viana, Takiya, Christina Maeda, Pinhão, Angela Teixeira, Schatzmayr, Hermann Gonçalves
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/38914
Acceso en línea:https://arca.fiocruz.br/handle/icict/38914
Access Level:acceso abierto
Palabra clave:Vírus da Dengue tipo 2
Fígado
Viremia
Ultraestrutura
Histopatologia
Dengue-2 virus
BALB/c mice
Liver
Ultrastructure
Hispopathology
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oai_identifier_str oai:arca.fiocruz.br:icict/38914
network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.none.fl_str_mv Morphological studies in a model for dengue-2 virus infection in mice
title Morphological studies in a model for dengue-2 virus infection in mice
spellingShingle Morphological studies in a model for dengue-2 virus infection in mice
Barth, Ortrud Monika
Vírus da Dengue tipo 2
Fígado
Viremia
Ultraestrutura
Histopatologia
Dengue-2 virus
BALB/c mice
Liver
Viremia
Ultrastructure
Hispopathology
title_short Morphological studies in a model for dengue-2 virus infection in mice
title_full Morphological studies in a model for dengue-2 virus infection in mice
title_fullStr Morphological studies in a model for dengue-2 virus infection in mice
title_full_unstemmed Morphological studies in a model for dengue-2 virus infection in mice
title_sort Morphological studies in a model for dengue-2 virus infection in mice
dc.creator.none.fl_str_mv Barth, Ortrud Monika
Barreto, Débora Ferreira
Paes, Marciano Viana
Takiya, Christina Maeda
Pinhão, Angela Teixeira
Schatzmayr, Hermann Gonçalves
author Barth, Ortrud Monika
author_facet Barth, Ortrud Monika
Barreto, Débora Ferreira
Paes, Marciano Viana
Takiya, Christina Maeda
Pinhão, Angela Teixeira
Schatzmayr, Hermann Gonçalves
author_role author
author2 Barreto, Débora Ferreira
Paes, Marciano Viana
Takiya, Christina Maeda
Pinhão, Angela Teixeira
Schatzmayr, Hermann Gonçalves
author2_role author
author
author
author
author
dc.subject.other.none.fl_str_mv Vírus da Dengue tipo 2
Fígado
Viremia
Ultraestrutura
Histopatologia
topic Vírus da Dengue tipo 2
Fígado
Viremia
Ultraestrutura
Histopatologia
Dengue-2 virus
BALB/c mice
Liver
Viremia
Ultrastructure
Hispopathology
dc.subject.en.none.fl_str_mv Dengue-2 virus
BALB/c mice
Liver
Viremia
Ultrastructure
Hispopathology
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2019-12-31T18:57:21Z
dc.date.available.fl_str_mv 2019-12-31T18:57:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARTH, Ortrud Monika et al. Morphological studies in a model for dengue-2 virus infection in mice. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 8. p. 905-915, Dec. 2006.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/38914
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762006000800014
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BARTH, Ortrud Monika et al. Morphological studies in a model for dengue-2 virus infection in mice. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 8. p. 905-915, Dec. 2006.
0074-0276
10.1590/s0074-02762006000800014
1678-8060
url https://arca.fiocruz.br/handle/icict/38914
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fiocruz/IOC
publisher.none.fl_str_mv Fiocruz/IOC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2019-12-31T18:57:21Z2019-12-31T18:57:21Z2006BARTH, Ortrud Monika et al. Morphological studies in a model for dengue-2 virus infection in mice. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 8. p. 905-915, Dec. 2006.0074-0276https://arca.fiocruz.br/handle/icict/3891410.1590/s0074-027620060008000141678-8060engFiocruz/IOCVírus da Dengue tipo 2FígadoViremiaUltraestruturaHistopatologiaDengue-2 virusBALB/c miceLiverViremiaUltrastructureHispopathologyMorphological studies in a model for dengue-2 virus infection in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Histologia e Embriologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.One of the main difficulties in studying dengue virus infection in humans and in developing a vaccine is the absence of a suitable animal model which develops the full spectrum of dengue fever, dengue haemorrhagic fever, and dengue shock syndrome. It is our proposal to present morphological aspects of an animal model which shows many similarities with the dengue infection in humans. BALB/c mice were intraperitoneally infected with non-neuroadapted dengue virus serotype 2 (DENV-2). Histopathological and morphometrical analyses of liver tissue revealed focal alterations along the infection, reaching wide-ranging portal and centrolobular veins congestion and sinusoidal cell death. Additional ultrastructural observations demonstrated multifocal endothelial injury, platelet recruitment, and alterated hepatocytes. Dengue virus antigen was detected in hepatocytes and in the capillar endothelium of the central lobular vein area. Liver function tests showed high levels of aspartate transaminase and alanine transaminase enzyme activity. Lung tissue showed interstitial pneumonia and mononuclear cells, interseptal oedema, hyperplasia, and hypertrophy of the bronchiolar epithelial cells. DENV-2 led to a transient inflammatory process, but caused focal alterations of the blood-exchange barrier. 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