Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis

Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.

Bibliographic Details
Authors: Nascimento, Ana Paula Barbosa do, Medeiros Filho, Fernando, Pauer, Heidi, Antunes, Luis Caetano Martha, Sousa, Hério, Senger, Hermes, Albano, Rodolpho Mattos, Santos, Marcelo Trindade dos, Carvalho-Assef, Ana Paula D’alincourt, Silva, Fabricio Alves Barbosa da
Format: article
Status:Published version
Publication Date:2020
Country:Brasil
Institution:Fundação Oswaldo Cruz (FIOCRUZ)
Repository:Repositório Institucional da FIOCRUZ (ARCA)
Language:English
OAI Identifier:oai:arca.fiocruz.br:icict/46046
Online Access:https://arca.fiocruz.br/handle/icict/46046
Access Level:Open access
Keyword:Pseudomonas aeruginosa
SPM-1
Produtoras de metalo-beta-lactamase
Genômica comparativa
Análise fenotípica
SPM‑1
Metallo‑beta‑lactamase‑producing
Comparative genomics
Phenotypic analysis
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network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.none.fl_str_mv Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis
title Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis
spellingShingle Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis
Nascimento, Ana Paula Barbosa do
Pseudomonas aeruginosa
SPM-1
Produtoras de metalo-beta-lactamase
Genômica comparativa
Análise fenotípica
SPM‑1
Metallo‑beta‑lactamase‑producing
Pseudomonas aeruginosa
Comparative genomics
Phenotypic analysis
title_short Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis
title_full Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis
title_fullStr Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis
title_full_unstemmed Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis
title_sort Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis
dc.creator.none.fl_str_mv Nascimento, Ana Paula Barbosa do
Medeiros Filho, Fernando
Pauer, Heidi
Antunes, Luis Caetano Martha
Sousa, Hério
Senger, Hermes
Albano, Rodolpho Mattos
Santos, Marcelo Trindade dos
Carvalho-Assef, Ana Paula D’alincourt
Silva, Fabricio Alves Barbosa da
author Nascimento, Ana Paula Barbosa do
author_facet Nascimento, Ana Paula Barbosa do
Medeiros Filho, Fernando
Pauer, Heidi
Antunes, Luis Caetano Martha
Sousa, Hério
Senger, Hermes
Albano, Rodolpho Mattos
Santos, Marcelo Trindade dos
Carvalho-Assef, Ana Paula D’alincourt
Silva, Fabricio Alves Barbosa da
author_role author
author2 Medeiros Filho, Fernando
Pauer, Heidi
Antunes, Luis Caetano Martha
Sousa, Hério
Senger, Hermes
Albano, Rodolpho Mattos
Santos, Marcelo Trindade dos
Carvalho-Assef, Ana Paula D’alincourt
Silva, Fabricio Alves Barbosa da
author2_role author
author
author
author
author
author
author
author
author
dc.subject.other.none.fl_str_mv Pseudomonas aeruginosa
SPM-1
Produtoras de metalo-beta-lactamase
Genômica comparativa
Análise fenotípica
topic Pseudomonas aeruginosa
SPM-1
Produtoras de metalo-beta-lactamase
Genômica comparativa
Análise fenotípica
SPM‑1
Metallo‑beta‑lactamase‑producing
Pseudomonas aeruginosa
Comparative genomics
Phenotypic analysis
dc.subject.en.none.fl_str_mv SPM‑1
Metallo‑beta‑lactamase‑producing
Pseudomonas aeruginosa
Comparative genomics
Phenotypic analysis
description Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-02-11T19:05:10Z
dc.date.available.fl_str_mv 2021-02-11T19:05:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NASCIMENTO, Ana Paula Barbosa do et al. Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis. Scientific Reports, v. 20, 13192, 16p, 2020.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/46046
dc.identifier.issn.none.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-020-69944-6
identifier_str_mv NASCIMENTO, Ana Paula Barbosa do et al. Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis. Scientific Reports, v. 20, 13192, 16p, 2020.
2045-2322
10.1038/s41598-020-69944-6
url https://arca.fiocruz.br/handle/icict/46046
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2021-02-11T19:05:10Z2021-02-11T19:05:10Z2020NASCIMENTO, Ana Paula Barbosa do et al. Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis. Scientific Reports, v. 20, 13192, 16p, 2020.2045-2322https://arca.fiocruz.br/handle/icict/4604610.1038/s41598-020-69944-6engNature ResearchPseudomonas aeruginosaSPM-1Produtoras de metalo-beta-lactamaseGenômica comparativaAnálise fenotípicaSPM‑1Metallo‑beta‑lactamase‑producingPseudomonas aeruginosaComparative genomicsPhenotypic analysisCharacterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz; Escola Nacional de Saúde Pùblica Sergio Arouca. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Carlos. Departamento de Computação. São Carlos, SP, Brasil.Universidade Federal de São Carlos. Departamento de Computação. São Carlos, SP, Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Pseudomonas aeruginosa is one of the most common pathogens related to healthcare-associated infections. The Brazilian isolate, named CCBH4851, is a multidrug-resistant clone belonging to the sequence type 277. The antimicrobial resistance mechanisms of the CCBH4851 strain are associated with the presence of the blaSPM-1 gene, encoding a metallo-beta-lactamase, in combination with other exogenously acquired genes. Whole-genome sequencing studies focusing on emerging pathogens are essential to identify key features of their physiology that may lead to the identification of new targets for therapy. Using both Illumina and PacBio sequencing data, we obtained a single contig representing the CCBH4851 genome with annotated features that were consistent with data reported for the species. However, comparative analysis with other Pseudomonas aeruginosa strains revealed genomic differences regarding virulence factors and regulatory proteins. In addition, we performed phenotypic assays that revealed CCBH4851 is impaired in bacterial motilities and biofilm formation. On the other hand, CCBH4851 genome contained acquired genomic islands that carry transcriptional factors, virulence and antimicrobial resistance-related genes. Presence of single nucleotide polymorphisms in the core genome, mainly those located in resistance-associated genes, suggests that these mutations may also influence the multidrug-resistant behavior of CCBH4851. Overall, characterization of Pseudomonas aeruginosa CCBH4851 complete genome revealed the presence of features that strongly relates to the virulence and antibiotic resistance profile of this important infectious agent.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZNascimento, Ana Paula Barbosa doMedeiros Filho, FernandoPauer, HeidiAntunes, Luis Caetano MarthaSousa, HérioSenger, HermesAlbano, Rodolpho MattosSantos, Marcelo Trindade dosCarvalho-Assef, Ana Paula D’alincourtSilva, Fabricio Alves Barbosa daLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/7dce188e-55ef-4fb3-936b-7677eac5d920/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALAnaPaulaAlincourtAssef_etal_IOC_2020.pdfAnaPaulaAlincourtAssef_etal_IOC_2020.pdfapplication/pdf2224550https://arca.fiocruz.br/bitstreams/90f033aa-7aa8-4482-9309-5d0c1589d26b/download75e5933a5ff031d3d8b6f66075858e65MD52trueAnonymousREADTEXTAnaPaulaAlincourtAssef_etal_IOC_2020.pdf.txtAnaPaulaAlincourtAssef_etal_IOC_2020.pdf.txtExtracted texttext/plain71917https://arca.fiocruz.br/bitstreams/e8e5a2de-9134-47d4-bd50-96895e1e0a58/downloade87a1505abcc189cba8d595ddbeffed8MD59falseAnonymousREADTHUMBNAILAnaPaulaAlincourtAssef_etal_IOC_2020.pdf.jpgAnaPaulaAlincourtAssef_etal_IOC_2020.pdf.jpgGenerated Thumbnailimage/jpeg45490https://arca.fiocruz.br/bitstreams/d3457e44-4357-410b-9feb-0894c757ad1a/download5015b4e4dae9f573ea1114f86052c754MD510falseAnonymousREADicict/460462025-12-10 15:14:59.859open.accessoai:arca.fiocruz.br:icict/46046https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T18:14:59Repositório Institucional da FIOCRUZ (ARCA) - 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