Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets

tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approache...

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Detalles Bibliográficos
Autores: Morais, Guilherme Loss de, Waterhouse, Peter M., Margis, Rogerio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Institución:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:inglés
OAI Identifier:oai:www.lume.ufrgs.br:10183/110062
Acceso en línea:http://hdl.handle.net/10183/110062
Access Level:acceso abierto
Palabra clave:Arabidopsis thaliana
tRNAs
Small RNA
tRFs
tRNA-derived RNA fragments
Argonaute and Arabidopsis
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spelling 2015-02-12T02:15:46Z20131745-6150http://hdl.handle.net/10183/110062000893284tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants.application/pdfengBiology direct. London. Vol. 8, no. 6 (17 May 2013), 5 p.Arabidopsis thalianatRNAsSmall RNAtRFstRNA-derived RNA fragmentsArgonaute and ArabidopsisDescription of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targetsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSMorais, Guilherme Loss deWaterhouse, Peter M.Margis, RogerioORIGINAL000893284.pdf000893284.pdfTexto completo (inglês)application/pdf983753http://www.lume.ufrgs.br/bitstream/10183/110062/1/000893284.pdf35a7e23a2bb1a44414117a5c103d7840MD51TEXT000893284.pdf.txt000893284.pdf.txtExtracted Texttext/plain19094http://www.lume.ufrgs.br/bitstream/10183/110062/2/000893284.pdf.txtecab38205c45109c092220da0fbd7489MD52THUMBNAIL000893284.pdf.jpg000893284.pdf.jpgGenerated Thumbnailimage/jpeg2059http://www.lume.ufrgs.br/bitstream/10183/110062/3/000893284.pdf.jpg916352f0d594caa0cd1c718841cebe94MD5310183/1100622018-10-23 09:17:34.592oai:www.lume.ufrgs.br:10183/110062Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2018-10-23T12:17:34Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
spellingShingle Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
Morais, Guilherme Loss de
Arabidopsis thaliana
tRNAs
Small RNA
tRFs
tRNA-derived RNA fragments
Argonaute and Arabidopsis
title_short Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_full Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_fullStr Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_full_unstemmed Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_sort Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
dc.creator.none.fl_str_mv Morais, Guilherme Loss de
Waterhouse, Peter M.
Margis, Rogerio
author Morais, Guilherme Loss de
author_facet Morais, Guilherme Loss de
Waterhouse, Peter M.
Margis, Rogerio
author_role author
author2 Waterhouse, Peter M.
Margis, Rogerio
author2_role author
author
dc.subject.por.fl_str_mv Arabidopsis thaliana
topic Arabidopsis thaliana
tRNAs
Small RNA
tRFs
tRNA-derived RNA fragments
Argonaute and Arabidopsis
dc.subject.eng.fl_str_mv tRNAs
Small RNA
tRFs
tRNA-derived RNA fragments
Argonaute and Arabidopsis
description tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-02-12T02:15:46Z
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/110062
dc.identifier.issn.pt_BR.fl_str_mv 1745-6150
dc.identifier.nrb.pt_BR.fl_str_mv 000893284
identifier_str_mv 1745-6150
000893284
url http://hdl.handle.net/10183/110062
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Biology direct. London. Vol. 8, no. 6 (17 May 2013), 5 p.
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